7
Pre-rinse the tips
Some liquids (e.g. protein-containing solutions and
organic solvents) can leave a film of liquid on the inside
wall of the tip ; pre-rinse the tip to minimize any errors
that may be related to this phenomenon.
Pre-rinsing consists of aspirating the first volume of liquid
and then dispensing it back into the same vessel (or to
waste). Subsequent volumes that you pipette will have
levels of accuracy and precision within specifications.
