Pipetting
Place a vessel containing the solution to be pipetted in the front location of the right
drawer. The solution should be under the pipetting head.
Liquids containing protein solutions and organic solvents can leave a film of liquid
on the inside wall of the tip; pre-rinse the tip to minimize any errors related to
this phenomenon. Pre-rinsing consists of aspirating the first volume of liquid and
then dispensing it back into the same vessel (or into a waste basket). Subsequent
volumes pipetted will have levels of accuracy and precision within specifications. As
recommended by the ISO 8655 standard, pre-rinse to stabilize dead volume inside
the pipetting head
ASPIRATION
•
Gently squeeze the pipetting handle
to the first stop
(this corresponds to the
set volume of liquid).
•
Use the pipetting handle to lower
the pipetting head
to immerse the
tips in the liquid (see immersion depth
table below).
It is important that the
tips do not touch the bottoms of the
wells.
•
Slowly retract the pipetting handle to
aspirate the liquid into the tips.
•
Move the pipetting head fully up.
To simplify this operation, the height
adjuster
can be set by turning the
upper screw so that the pipetting head
may only be moved down to a position
slightly above the bottom.
MODEL
IMMERSION DEPTH
(MILLIMETERS)
WAIT TIME
(SECONDS)
P20
2-3
1
P220
2-4
1
Check the tip ejector when fitting tips. If the tips have shifted this
implies that they have not been fitted correctly.
The maximum
immersion depth
of the tips can be
adjusted using a
height adjuster.
1
2
3
1
1
2
3
NOTE
NOTE
O
PE
R
A
TIO
N
9
PLATEMASTER®
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USER’S GUIDE
Summary of Contents for F110761
Page 1: ...User s Guide EN...
Page 18: ...NOTES Notes 18 Notes PLATEMASTER...
