Action
Step
In the Sample Application phase, choose an appropriate sample application
technique and volume. See
Section 6.2 Prepare sample for loading, on page 82
for suitable sample application techniques for your sample volume.
5
Tip:
If you are using a sample loop, empty the loop with 3 to 5 volumes of buffer.
This will make sure that the loop is completely emptied.
Note:
For sample application using a Superloop, refer to the ÄKTA go User Manual.
In the Column wash phase, choose a suitable volume and concentration of
buffer B, and whether a fraction collection is to be performed.
6
In the Elution phase, set how to elute the sample, the duration of the elution,
the concentration of buffer B, and how the eluted sample will be collected.
7
In the Re-equilibration phase, choose a suitable volume and concentration
of buffer B.
8
Click the save button, choose the system, name, and location for your method
and click Save.
9
It is advisable to run a blank run, without sample, before running the method
with sample. This makes sure that the column is clean and that the method
and the system are set up properly.
Tip:
ÄKTA go Operating Instructions 29360951 AC
81
6 Run a method
6.1 Create a method
