5.4
Connect a column
Introduction
This section describes how to connect a column to the instrument using a column
holder and without introducing air into the flow path. Several types of column holders
are available for the ÄKTA avant instrumen, see ÄKTA avant User Manual.
WARNING
Before connecting a column, read the instructions for use of the
column. To avoid exposing the column to excessive pressure, make
sure that the pressure limit is set to the specified maximum pressure
for the column.
Methods automatically include pressure alarms based on the specifications of the chosen
column type. However, when running manual runs you have to set the pressure limits
yourself. Also, to protect the column media, special settings are needed. See
Set pressure alarms, on page 117
for more information on pressure alarms.
Do not overtighten when connecting columns. Overtightening might rupture
the connectors or squeeze the tubing and thereby result in high back pressure.
Note:
112
ÄKTA avant Operating Instructions 29101556 AB
5 Prepare the system for a run
5.4 Connect a column
