GE AKTA avant Operating Instructions Manual Download

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Description

Part

System pump A

Pressure monitor

Mixer

Injection valve

Sample loop or Superloop

Column valve

Column

UV monitor

Conductivity monitor

Flow restrictor

pH valve with pH monitor

Outlet valve

Fraction collector

Prepare the inlet tubing

Connect inlet tubing to the inlet ports that are to be used, and immerse all inlet tubing
that is to be used during the method run in the correct buffers.

108

ÄKTA avant Operating Instructions 29101556 AB

5 Prepare the system for a run

5.2 Prepare the flowpath

Summary of Contents for AKTA avant

Page 1: ...KTA avant Operating Instructions Original instructions...

Page 2: ...nstallation 52 4 1 Site preparation 53 4 1 1 Delivery and storage 55 4 1 2 Room requirements 58 4 1 3 Site environment 60 4 1 4 Power requirements 61 4 1 5 Computer requirements 63 4 1 6 Required mate...

Page 3: ...ocedures 148 7 Maintenance 149 7 1 Maintenance program 152 7 2 Replace pump piston seal O rings and rinse membrane of Pump P9 or Pump P9H 163 7 3 Replace pump piston seal O ring and rinse membrane of...

Page 4: ...egula tory information intended use of the KTA avant instrument and lists of associated documentation In this chapter See page Section 5 1 1 About this manual 6 1 2 Important user information 8 1 3 Re...

Page 5: ...uments using previously created methods in UNICORN software 6 3 2 and later Typographical conventions Software items are identified in the text by bold italic text A colon separates items in a group f...

Page 6: ...ion of biomolecules The system can be used to screen for optimal choice of columns media and running parameters to purify selected proteins The KTA avant system is intended for research use only and s...

Page 7: ...zardous situation which if not avoided could result in minor or moderate injury It is important not to pro ceed until all stated conditions are met and clearly understood NOTICE NOTICE indicates instr...

Page 8: ...turing information Information Requirement GE Healthcare Bio Sciences AB Name and address of manufacturer Bj rkgatan 30 SE 751 84 Uppsala Sweden In this section See page Section 9 1 3 1 EU Directives...

Page 9: ...ty EMC Directive 2014 30 EU Low Voltage Directive LVD 2014 35 EU Restriction of Hazardous Substances RoHS Directive 2011 65 EU Radio Equipment Directive RED 2014 53 EU CE marking The CE marking and th...

Page 10: ...formation Requirement See Manufacturing information Name and address of manufacturer Telephone 46 771 400 600 Telephone number of manufacturer GE Healthcare LLC Importer and or company for obtain ing...

Page 11: ...pressly approved by GE could void the user s authority to operate the equipment Note This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to part 15...

Page 12: ...t are summarized in the table below Description Standard Safety of machinery General principles for design Risk assessment and risk reduction EN ISO 12100 Safety requirements for electrical equipment...

Page 13: ...Electromagnetic compatibility and Radio spectrum Matters ERM ElectroMagnetic Compatibility EMC standard for radio equipment and services Part 3 Spe cific conditions for Short Range Devices SRD operati...

Page 14: ...ions for handling the system Descrip tions of components Information about how to run and maintain the system KTA avant User Manual 29035184 System specification and declaration of ma terial conformit...

Page 15: ...the Evaluation Classic module in UNICORN Description of the evaluation algorithms used in UNICORN UNICORN Evaluation Manual1 Overview and detailed description of the system control features in UNICORN...

Page 16: ...actical tips on chromatography refer to KTA Laboratory scale Chromatography Systems Instrument Management Handbook product code 29010831 16 KTA avant Operating Instructions 29101556 AB 1 Introduction...

Page 17: ...ion Important WARNING Before installing operating or maintaining the product all users must read and understand the entire contents of this chapter to become aware of the hazards involved In this chap...

Page 18: ...age 21 System operation on page 23 Maintenance on page 25 General precautions WARNING Risk assessment Perform a risk assessment for any risks due to the process or process environment Evaluate the eff...

Page 19: ...void condensation If KTA avant is kept in a cold room cold cabinet or similar keep it switched on in order to avoid condensa tion Personal protection WARNING To avoid injury when working with the KTA...

Page 20: ...ipment Close doors To minimize the risk of exposure to hazardous chemicals and pressurized liquids always close the foldable door and the pump cover before starting a run Cut injuries The tubing cutte...

Page 21: ...d moving WARNING To avoid injury when installing and moving the KTA avant system take the following measures for personal protection Move transport crates Make sure that the lifting equipment has the...

Page 22: ...rs The maximum level of the waste vessel for the waste tubing from the valves must be lower than 30 cm above the lab bench The maximum level of the waste vessel for the waste tubing from the fraction...

Page 23: ...es always place bottles on the buffer tray and close the doors before rotating the instrument Fasten bottles and cassettes Always fasten bottles and cas settes to the rails at the front and side panel...

Page 24: ...the KTA avant system follow these instructions Risk of breaking test vials Do not use excessive force to press vials of wrong dimensions into the fraction collector cassettes Glass vials may break and...

Page 25: ...kept in a cold cabinet and the cold cabinet is switched off make sure to switch off KTA avant and keep the cold cabinet open to avoid overheating Place the computer in room temperature If the KTA ava...

Page 26: ...strong base or acid flush with water afterwards and wash with a weak neutral buffer solution in the last step or phase NOTICE To avoid damage to the KTA avant instrument or other equipment when perfor...

Page 27: ...her safety or regulatory labels that are attached to the product Labels on the KTA avant instrument The following illustration shows the labels that are attached to the KTA avant instrument KTA avant...

Page 28: ...lammable liquids in the built in fraction collector When running RPC methods collect fractions through the outlet valve or the optional external Fraction collector F9 R Warning Read the Operating Inst...

Page 29: ...N CSA C22 2 No 61010 1 Eurasian Conformity mark the single conformity mark indicates that the product is approved for circulation on the markets of the member states of the Eurasian Customs Union CAN...

Page 30: ...ergency situation stop the run by either pausing the run or switching off the instrument as described in the following table then If you want to Press the Pause button on the instrument display This w...

Page 31: ...before the power failure is available in UNICORN KTA avant instrument The UNICORN computer shuts down The instrument display shows status Not connected The run is interrupted immediately Data generat...

Page 32: ...after emergency shutdown or power failure Follow the instructions to restart the instrument after an emergency shutdown or power failure Action Step Make sure that the condition that caused the emerge...

Page 33: ...ated and recycled according to national and local environmental regulations CAUTION Always use appropriate personal protective equipment when de commissioning the equipment Disposal of electrical comp...

Page 34: ...itions so that the use of such electrical and electronic products will not result in any severe environmen tal pollution any bodily injury or damage to any assets The unit of the period is Year In ord...

Page 35: ...11358 0 GB T26572 X GB T 26572 0 Indicates that this hazardous substance contained in all of the homogeneous materials for this part is below the limit requirement in GB T 26572 X Indicates that this...

Page 36: ...chapter This chapter contains the following sections See page Section 37 3 1 KTA avant instrument overview 46 3 2 UNICORN software Illustration of the system The following illustration shows the KTA a...

Page 37: ...placed on the right hand side of the instrument This side can be covered by a foldable door and a pump cover By rotating the instrument using the swivel foot any side is easily accessed Operating rang...

Page 38: ...ent Function Part Function Part Buffer tray 2 Fraction collector 1 Wet side 4 Instrument display 3 Pump cover 6 Foldable door 5 Swivel foot lock unlock knob 8 Holder rails 7 Swing out toolbox 10 Swive...

Page 39: ...alve 3 Conductivity monitor 6 UV monitor 5 Quaternary valve 8 Inlet valve B 7 Pressure monitor of system pumps 10 System pump B 9 Inlet valve A 12 System pump flow restrictor 11 Mixer 14 System pump A...

Page 40: ...25 will give too high back pressure if used above 50 ml min The larger volumes in the H valves for KTA avant 150 may decrease resolution and increase peak broadening if used in KTA avant 25 Note Stan...

Page 41: ...1 5 External air sensor L9 1 5 L9 1 2 L9 1 2 External air sensor L9 1 2 E9 E9 I O box U9 L U9 L Second UV monitor C9 C9 Second Conductivity monitor F9 R F9 R Second Fraction collector Description of s...

Page 42: ...Available volumes are 1 4 ml 5 ml mounted at delivery and 15 ml CAUTION Risk of explosion Do not use Mixer chamber 15 ml with an KTA avant 25 sys tem configuration The maximum pressure for Mix er cha...

Page 43: ...let valve V9 O or V9H O Monitor which measures the UV Vis absorbance at up to three wavelengths simultaneously in the range 190 to 700 nm UV monitor U9 M Monitorwhichcontinuouslymeasurestheconduc tivi...

Page 44: ...flow direction through the column or to by pass the column Second Column valve V9 C2 or V9H C2 Valve which adds 12 outlet ports to the system giving a total of 21 outlets Second Outlet valve V9 O2 or...

Page 45: ...em status The in strument display includes the following indicators and buttons Description Indicator Button Indicates if the Instrument display buttons are unlocked or locked The buttons can be locke...

Page 46: ...learn more about System Control and the other three modules Administration Method Editor and Evaluation see the UNICORN documentation package In this section See page Section 47 3 2 1 UNICORN softwar...

Page 47: ...one or a combination of Method Editor Predefined methods with built in application support Drag and drop functionto buildmethodswithrelevant steps Line by line text editing The interface provides easy...

Page 48: ...d Evaluation Classic it is possible to access descriptions of the active window by pressing the F1 key This can be especially helpful when editing methods 48 KTA avant Operating Instructions 29101556...

Page 49: ...rent data in numerical values The Chromatogram pane 2 illustrates data as curves during the entire run The current flow path is illustrated in the Process Picture 3 which allows manual inter actions w...

Page 50: ...the method run while current flow rate and valve positions are sustained End Permanently ends the method run Continue Resumes for ex ample a held or paused method run ConnecttoSystems Opens the Connec...

Page 51: ...w to how to unpack the KTA avant instrument and how to lift the instrument onto a laboratory bench see KTA avant Unpacking In structions Note In this chapter This chapter contains the following sectio...

Page 52: ...ite must be planned and prepared before installing the KTA avant system The performance specifications of the system can be met only if the laboratory environment fulfills the requirements stated in t...

Page 53: ...Inform your GE representative of such damage Move the delivery box to a protected location indoors Delivery box KTA avant instruments are shipped in a delivery box with the following dimensions and we...

Page 54: ...ccommodatethesizeandweight of the instrument Cart for transporting the instru ment to the lab Unpacking the KTA avant instrument For information on how to how to unpack the KTA avant instrument and ho...

Page 55: ...switch must always be easy to access The power cord with plug must always be easy to disconnect Transportation route Doors corridors and elevators must have a minimum width of 75 cm to allow for tran...

Page 56: ...foot When ro tating the instrument take care not to stretch or squeeze tubing or cables A disconnected cable may cause power interruption or network interruption Stretched tubing may cause bottles to...

Page 57: ...following illustration 66 cm 86 cm 71 cm Equipment weight Weight Item 116 kg KTA avant instrument approximately 9 kg Computer approximately 3 kg Monitor approximately 130 kg Total KTA avant Operating...

Page 58: ...e kept to a minimum The installation site must comply with the following specifications Environmental requirements Requirement Parameter Indoor use only Allowed location 4 C to 35 C Ambient temperatur...

Page 59: ...W KTA avant instrument Maximum 800 W Typically 300 W Computer incl monitor and printer Refer to manufacturer s instructions for more information Typically 700 W Total heat output Maximum 1100 W KTA a...

Page 60: ...nded mains sockets fused or protect ed by equivalent circuit breaker Type of sockets Maximum 2 m from the instrument due to length of mains cable Extension cables can be used if required Location of s...

Page 61: ...Intel Dual Core or faster Intel Dual Core or faster Intel Dual Core or faster Architecture Note UNICORN is tested using the English U S Code 1033 operating system language version Using other language...

Page 62: ...interface to be scaled The interface scale must remain at 100 to avoid issues with clipping and misaligning of parts of the UNICORN user interface Normally the scale is set at 100 by default 62 KTA a...

Page 63: ...test and system test 1 liter Distilled water Quaternary Valve test 0 5 liter 1 acetone in distilled water System test 0 5 liter 1 acetone and 1 M NaCl in distilled water Priming of the pump piston rin...

Page 64: ...VWR 102 96 13 3 12 8 BD Biosciences 120 114 17 16 15 BD Biosciences 116 110 30 28 50 Nalgene Kautex 121 L 64 5 W 641 L 55 W 551 250 ml bottle 1 Length and width of the rectangular bottle base Deep we...

Page 65: ...01 5200 GE 96 deep well plate 353966 BD Biosciences 780270 Greiner Bio One 219009 Porvair Sciences S30009 Seahorse Bioscience 951033405 0030501 306 Eppendorf 7701 5500 GE 48 deep well plate S30004 Sea...

Page 66: ...er cords with approved plugs delivered or approved by GE WARNING Access to power switch and power cord with plug Do not block access to the power switch and power cord The power switch must always be...

Page 67: ...See page Section 80 4 2 6 Start the instrument and the computer KTA avant Operating Instructions 29101556 AB 67 4 Installation 4 2 Hardware installation...

Page 68: ...lling Unpack and install the computer according to the manufacturer s instructions NOTICE Any computer used with the equipment must comply with IEC 60950 and be installed and used according to the man...

Page 69: ...NG Power cord Only use power cords with approved plugs deliv ered or approved by GE Supply voltage Before connecting the power cord make sure that the supply voltage at the wall outlet corresponds to...

Page 70: ...by authorized service engineers only NOTICE Misuse of UniNet 9 connectors The UniNet 9 connectors at the rear panel should not be mistaken for Firewire connectors Do not connect any external equipment...

Page 71: ...nused power cord Connect the power cord to the Power input connector on the back of the instrument and to a grounded wall outlet 100 to 240 VAC 50 to 60 Hz 2 Attach the power cord to the rear of the i...

Page 72: ...connector 1 If the computer is to be connected to an external network connect a network cable between the main network card of the computer and a network wall outlet Note If the computer has not been...

Page 73: ...illustration 1 2 Description Part Waste tubing from the injection valve the pH valve and the outlet valve pieces of tubing marked W W1 W2 and W3 1 Waste tubing from the fraction collector and the buff...

Page 74: ...h Place the three pieces of waste tubing from the fraction collector and the buffer tray in a waste vessel placed below the bench 2 NOTICE The maximum level of the waste vessel for the waste tubing fr...

Page 75: ...mber Note CAUTION Make sure that the waste vessels will hold all the produced volume of the run For KTA avant 25 a suitable waste vessel should typi cally have a volume of 2 to 10 liters For KTA avant...

Page 76: ...use injury Follow the instructions to install the pH electrode Action Step Unpack the pH electrode Make sure that the electrode is not broken or dry 1 Unscrew the dummy electrode from the flow cell 2...

Page 77: ...pump piston rinsing system 1 Outlet tubing from the sample pump piston rinsing system 2 Inlet tubing to the system pump piston rinsing system 3 Outlet tubing from the system pump piston rinsing system...

Page 78: ...he rinsing solution tubes back in the holders 3 Immerse the inlet tubing to the system pump piston rinsing system in one of the rinsing solution tubes Note Make sure that the inlet tubing reaches clos...

Page 79: ...ect a 25 to 30 ml syringe to the outlet tubing from the sample pump piston rinsing system Draw liquid slowly into the syringe 9 Disconnect the syringe and discard its contents 10 Immerse the outlet tu...

Page 80: ...he computer Action Step Switch on the instrument by pressing the Power switch to the I position 1 Result The instrument starts and the Instrument display states Not connect ed Turn on the computer and...

Page 81: ...the UNICORN Administration and Technical Manual Software installations You can install UNICORN in one of the following configurations as a complete UNICORN installation on a stand alone workstation fu...

Page 82: ...ation and Technical Manual Start UNICORN and log on Follow the instructions to start UNICORN and log on to the program A valid e license must be available for the workstation See UNICORN Administratio...

Page 83: ...nd enter a network ID in the User Name field click OK Result The selected UNICORN modules open Connect to system Follow the instructions to connect the instrument to UNICORN The system must have been...

Page 84: ...box 2 Select a system check box Click Control for that system Click OK Result The selected instrument can now be controlled by the software Tip If UNICORN is unable to connect to the selected instrume...

Page 85: ...ction describes how to prime the buffer inlets sample inlets and Q inlets and how to purge the system pumps and the sample pump In this section This section contains the following subsections See page...

Page 86: ...ystem is filled with fresh rinsing solution Note The procedures for purging the pump heads and priming the inlets using the Process Picture are described in the following topic It is also possible to...

Page 87: ...syringe to the purge valve of one of the pump heads of System Pump B Make sure that the syringe fits tightly into the purge connector 4 Open the purge valve by turning it counter clockwise about thre...

Page 88: ...sary to purge System Pump B However if the pressure signal indicated air bubbles left in the pump follow these in structions to purge both pump heads of System Pump B Action Step Make sure that the pi...

Page 89: ...Picture 4 Click the System pumps icon Set Conc B to 100 B and click Set B Set the System flow to 1 0 ml min for KTA avant 25 or 5 0 ml min for KTA avant 150 Click Set flow rate Result Only System Pum...

Page 90: ...Close the purge valve by turning it clockwise Disconnect the syringe and discard its contents 7 Connect the syringe to the purge valve on the right pump head of System Pump B and repeat steps 6 to 8...

Page 91: ...valve A icon Click the position of one of the inlets that will be used at the beginning of the run Result The inlet valve switches to the selected port In the Process Picture 2 Click the System pumps...

Page 92: ...to manual load position Make sure that the pump flow is on 2 In the Chromatogram pane 3 Check the PreC pressure curve If the PreC pressure do not stabilize within a few minutes there may be air left i...

Page 93: ...em is filled with fresh rinsing solution Note Prime sample inlets Follow the instructions below to fill all sample inlet tubing to be used in the run with appropriate buffer or sample solution Action...

Page 94: ...t the syringe fits tightly into the purge connector 5 Open the purge valve by turning it counter clockwise about three quarters of a turn Draw slowly with the syringe until the sample just passes the...

Page 95: ...ubbles left in the pump follow the instruction below to purge both the pump heads of the sample pump Action Step Make sure that all sample inlet tubing that is to be used during the method run is imme...

Page 96: ...lve of the sample pump Make sure that the syringe fits tightly into the purge connector 6 Open the purge valve by turning it counter clockwise about three quarters of a turn Draw 5 to 10 ml of liquid...

Page 97: ...pump by following the instructions in Validate prime or purge of System Pump A or B or Sample Pump on page92 10 End the run Click the End button in the System Control toolbar to end the run KTA avant...

Page 98: ...p Make sure that the pieces of inlet tubing marked A1 B1 and Q1 Q4 are im mersed in the correct buffers The A1 and B1 positions are used for pump synchronization and these lines should already be prim...

Page 99: ...Q2 Q3 and Q4 respectively by setting the respective Quaternary start concentration to 100 Tip The inlet tubing that is immersed in distilled water should be the last piece of inlet tubing to be primed...

Page 100: ...heads of each system pump have to be purged Action Step In the Manual instructions dialog box 1 Select Pumps and pressures Pump wash and click All in the BufferPro Q inlets list Result A simultaneous...

Page 101: ...ise Disconnect the syringe and discard its contents 6 Repeat steps 3 to 5 for the other three purge valves of the system pumps to get rid of air in all pump heads Keep the system flow running during t...

Page 102: ...e taking the KTA avant instrument into use run performance tests to check the function of the equipment See KTA avant User Manual for further instructions 102 KTA avant Operating Instructions 29101556...

Page 103: ...ge Section 104 5 1 Before you prepare the system 106 5 2 Prepare the flowpath 111 5 3 Prime buffer inlets and purge system pumps 112 5 4 Connect a column 117 5 5 Set pressure alarms 119 5 6 Calibrate...

Page 104: ...use any accessories not supplied or recommended by GE Fire Hazard Before starting the system make sure that there is no leakage Checklist Prepare the instrument and accessories according to the follow...

Page 105: ...5 MPa 50 bar in KTA avant 25 Always replace the green PEEK tubing between the used system pump and the pump pressure monitor with orange PEEK tubing i d 0 5 mm before running RPC with 100 acetonitril...

Page 106: ...bottles may cause injury Spilled liquid may cause fire hazard and personal injury Max weight on buffer tray Do not place containers with a volume of more than 10 liters each on the buffer tray The tot...

Page 107: ...6 7 8 9 10 11 12 13 14 15 16 17 19 20 W1 W2 W 3 2 1 18 4 5 Description Part Pressure monitor 1 Sample pump 2 Sample inlet valve 3 Quaternary valve 4 Inlet valve A 5 Inlet valve B 6 System pump B 7 KTA...

Page 108: ...6 Flow restrictor 17 pH valve with pH monitor 18 Outlet valve 19 Fraction collector 20 Prepare the inlet tubing Connect inlet tubing to the inlet ports that are to be used and immerse all inlet tubing...

Page 109: ...Valve Illustrations Valves and ports Injection Valve label V9 Inj and V9H Inj Waste ports W1 W2 pH Valve label V9 pH and V9H pH Waste port W3 Outlet Valve label V9 O and V9H O Waste port W KTA avant O...

Page 110: ...ctor is to be used make sure that tubing is connected between the outlet valve Frac port and the fraction collector and prepare the fraction collector Otherwise immerse the outlet tubing in suitable t...

Page 111: ...fill the buffer inlets with liquid Purge the system pumps remove air from the pump heads For instructions on how to prime the inlets and purge the system pumps see Section 4 5 Prime inlets and purge p...

Page 112: ...set to the specified maximum pressure for the column Methods automatically include pressure alarms based on the specifications of the chosen column type However when running manual runs you have to se...

Page 113: ...r The column is connected to two opposite parts of the column valve using appro priate tubing and connectors Action Step Attach an appropriate column holder to the rail on the instrument 1 Attach the...

Page 114: ...on 1 was chosen in the method to be run 3 In the Process Picture 4 Click the Column valve icon Click e g Position 1 and Down flow Click Set position Result The column valve switches to position 1 114...

Page 115: ...em flow of 0 2 ml min starts When buffer leaves the tubing on port 1A if port 1A was chosen in the method to be run in a continuous mode and the top part of the column is filled with buffer connect th...

Page 116: ...a continuous mode connect this piece of tubing to the column valve Use the port opposite to the one already connected to the column in this example port 2B 8 Click the End button in the System Contro...

Page 117: ...n the high pressure side When placing UV and or conductivity flow cells on the high pressure side of the column the UV flow cell has a maximum pressure limit of 2 MPa 20 bar and the conductivityflow c...

Page 118: ...anual instructions dialog box opens In the Instructions box select Alarms Alarm pre column pressure 2 Click Enabled in the Mode field 3 Enter the high pressure limit in the High alarm box Click Execut...

Page 119: ...each the operating temperature before use Do not run a system flow during pH calibration Note Calibrate the pH monitor CAUTION pH electrode Handle the pH electrode with care The glass tip may break an...

Page 120: ...andard buffer 9 Result Calibration date and time are displayed in the dialog box and also values for Calibrated electrode slope and Asymmetry potential at pH 7 Is the Calibrated electrode slope 80 and...

Page 121: ...the outlet valve or the optional external Fraction collector F9 R Available cassettes trays and racks The following Cassettes and racks are available Cassette 3 ml tubes for 40 tubes Cassette 5 ml tub...

Page 122: ...for tubes or bottles 1 See UNICORN System Control Manual Change the System Settings in UNICORN to set the fractionation mode and other settings for fraction collection 2 Available System Settings are...

Page 123: ...p well plates are rotated so that the well marked A1 is positioned above the A1 marking on the cassette 2 Close the cassettes that have the QuickRelease function 3 KTA avant Operating Instructions 291...

Page 124: ...the illustration faces the front of the tray marked with the GE monogram 4 Open the fraction collector drawer by pressing the handle upwards and pulling out the drawer 5 124 KTA avant Operating Instr...

Page 125: ...fter the door has been closed the fraction collector performs a Full scan of the cassette type code of each cassette to identify the cassette types see Cassette and tray identification on page 127 Pre...

Page 126: ...he fraction collector drawer Make sure that the front of the rack marked with the GE monogram faces the front of the drawer and is hooked onto the two pins 3 Close the drawer Make sure that it snaps i...

Page 127: ...which type of cassettes are used Quick scan is performed during the run to ensure that correct cassettes are placed in the fraction collector WARNING Moving parts in fraction collector Do not open th...

Page 128: ...compat ible computer or place the computer outside the cold room and use the Ethernet cable delivered with the instrument to connect to the computer When the instrument is kept in a cold room it is im...

Page 129: ...handle the system after the run In this chapter This chapter contains the following sections See page Section 130 6 1 Before you start 133 6 2 Applying the sample 136 6 3 Start a method run 142 6 4 Mo...

Page 130: ...al regulations for safe opera tion and maintenance of the product High pressure The product operates under high pressure Wear protective glasses and other required Personal Protective Equipment PPE at...

Page 131: ...ervice and maintenance Hold pause or stop the run At the end of a method the run stops automatically All pumps stop and an acoustic end signal sounds and End is displayed in the Run Log To interrupt a...

Page 132: ...r paused method run Note An ended method cannot be continued click the End button permanently end the run When ending a method run in advance it is possible to save the partial result Note 132 KTA ava...

Page 133: ...the sample application are described in the following table For detailed instructions and information regarding the different sample applications techniques see KTA avant User Manual Description Stag...

Page 134: ...hrough port W1 4 Sample application injection through a sample loop A sample loop is manually filled with sample using a syringe connected to the Injection Valve During the method run the sample is au...

Page 135: ...mple loop with a buffer volume that exceeds the volume of the loop This will ensure that the loop is completely emptied Tip KTA avant Operating Instructions 29101556 AB 135 6 Run a method 6 2 Applying...

Page 136: ...se and start a method The following instructions describe how to open a method and start a run Action Step Open the System Control module and click the Open Method Navigator button 1 Result The Method...

Page 137: ...was selected at method creation the Select Columns dialog box opens Continue with the steps outlined in the next topic If column logging was not chosen at installation of UNICORN and or no column typ...

Page 138: ...start a run Action Step Is the column to be used already registered 1 If No continue to step 2 If Yes continue to step 5 In the Select Columns dialog box click New 2 Result The first New Column dialo...

Page 139: ...on the col umn Press and hold the trigger to create a beam When the scanner beeps the column ID is registered and displayed in the dialog box Alternatively manually enter the column ID that you find...

Page 140: ...e menu Click a column type on the Column type menu Select the Set medium expiration date check box and click a date on the menu Click OK Tip Alias can be used for easy identification of a column Resul...

Page 141: ...umn ID Alternatively click Select ID and click the column individual to be used in the run from the menu Click OK Result The run starts All necessary actions occur automatically according to the metho...

Page 142: ...stem Control module on page 49 Monitor the run To interrupt a method during a run you may click the Hold Pause or End buttons in System Control A held or paused method run can be resumed by clicking t...

Page 143: ...llowing illustration and de scribed in the following table Modules without a fixed place in the system are shown in a panel below the process picture modules are called components in the process pictu...

Page 144: ...xample below shows the pop up toolbar for the Injection valve icon Instructions can be given from the pop up toolbar of each component icon To display a detailed picture with explanations for example...

Page 145: ...hases included in a chromatographic method Tip WARNING Corrosive chemicals during maintenance If the system or column is cleaned with a strong base or acid flush with water afterwards and wash with a...

Page 146: ...d pH electrode storage If pH monitoring will not be used for a week or longer perform one of the following ac tions Inject new storage solution into the pH flow cell Replace the pH electrode with the...

Page 147: ...on the File menu click Exit UNICORN exit UNICORN Result All open UNICORN modules close If an edited method or result is open and not saved when you try to exit or log off UNICORN you will see a warnin...

Page 148: ...for maintenance and re placement of parts WARNING Always use appropriate Personal Protective Equipment PPE during operation and maintenance of this product In this chapter This chapter contains the f...

Page 149: ...be done by service personnel authorized by GE Do not open any covers or replace parts unless specifically stated in the user documentation Periodic maintenance program The following periodic maintena...

Page 150: ...the UV monitor Calibrate pressure monitors Replace the mixer Replace O ring in mixer Replace the UV flow cell Replace the flow restrictor Replace inlet filters Wipe off excess oil from pump heads Clea...

Page 151: ...Maintenance action Replace pump rinsing system tubing Replace valve modules KTA avant Operating Instructions 29101556 AB 151 7 Maintenance 7 1 Maintenance program...

Page 152: ...lution volume Always make sure that sufficient spare components are available before attempt ing to replace a spare part Replacing spare parts Read the instructions carefully For example some individu...

Page 153: ...MPa 60 to 80 bar For Pump P9H Tubing giving a back pressure of 2 to 3 MPa 20 to 30 bar For Pump P9 P9 Seal kit 25 ml For Pump P9H P9H Seal kit 150 ml Illustrations The illustrations below show the pa...

Page 154: ...Star screws 11 O ring 5 Inlet check valve 12 Support washer 6 Star screws 13 Disassemble the pump head Action Step Make sure that no run is in progress on the instrument 1 Disconnect the tubing from...

Page 155: ...inlet manifold 3 Disconnect the tubing of the pump piston rinsing system 4 Unscrew the two screws of the front section of the pump head using a star screwdriver T20 and pull off the front section 5 K...

Page 156: ...l 2 located in the front section of the pump head Note Be careful not to scratch the metal surfaces 6 Pump P9H 2 1 Pump P9 2 1 Unscrew one of the two screws securing the pump membrane housing using a...

Page 157: ...iston and return spring when assembling the pump head 9 Clean the pump head and pump membrane housing in an ultrasonic bath If there are particles on any surfaces the check valves should be removed an...

Page 158: ...e back into the pump head 1 Note Always use Lubricant 56686700 when exchanging the O ring 3 x 1 mm Wet a new seal with 20 ethanol Place the new seal in the hole in the front section of the pump head a...

Page 159: ...of the support washer Make sure to tighten the screws fully 3 Pump P9H Pump P9 Wet a new O ring 21 4 x 1 6 mm with 20 ethanol Fit the O ring around the support washer 4 Pump P9H Pump P9 KTA avant Oper...

Page 160: ...4 Make sure that the new seal is wetted with 20 ethanol and then tighten both screws fully 5 Reconnect the tubing of the pump piston rinsing system 6 Reconnect the inlet manifold 7 Reconnect the tubi...

Page 161: ...n Click Insert Select Pumps and Pressures Gradient and set Target to 0 B for System Pump A or 100 B for system pump B For Pump P9 Select Pumps and Pressures System flow and set the Flow rate to 5 0 ml...

Page 162: ...ion Step Run the flow for 2 hours 6 Discard the used buffer 7 162 KTA avant Operating Instructions 29101556 AB 7 Maintenance 7 2 Replace pump piston seal O rings and rinse membrane of Pump P9 or Pump...

Page 163: ...ent spare components are available before attempt ing to replace a spare part Replacing spare parts Read the instructions carefully For example some individual parts of the pump head can be as sembled...

Page 164: ...tion The illustration below shows the parts of the pump heads of Pump P9 S 1 2 3 4 5 10 8 7 9 6 Description Part Description Part Piston seal 6 Piston 1 Outlet check valve 7 Return spring 2 O ring 8 D...

Page 165: ...connectors to the sides to release the inlet manifold 3 Disconnect the tubing of the pump piston rinsing system 4 Unscrew one of the two screws of the pump head using a hex wrench Un screw the second...

Page 166: ...and return spring and use a new piston and return spring when assembling the pump head 7 Unscrew the two screws securing the drain plate and the rinse chamber Lift off the drain plate and discard the...

Page 167: ...ction 7 5 Clean pump head check valves on page 173 10 Replace O ring piston seal and rinse membrane Action Step Unscrew the purge valve of the pump head Replace the O ring on the purge valve with a ne...

Page 168: ...chamber shall be facing upwards Wet a new membrane with 20 ethanol and place the membrane into the rinse chamber with the conical face upwards 3 Assemble the pump head Make sure to assemble the pump...

Page 169: ...em 4 Reconnect the inlet manifold 5 Reconnect the tubing to the pump head and reconnect the pump inlet tubing 6 Break in the new pump piston seal see instruction below 7 Break in the new pump piston s...

Page 170: ...lick Insert Select Flow path Injection valve and click Direct inject on the Position menu Click Insert Select Pumps Sample flow and set the Flow rate to 25 0 ml min Click Insert Click Execute Result A...

Page 171: ...tion volume Always make sure that sufficient spare components are available before attempt ing to replace a spare part Replacing spare parts Read the instructions carefully For example some individual...

Page 172: ...seal O rings and rinse membrane of Pump P9 or Pump P9H on page 152 Replace pump pistons of Pump P9 S If a damaged piston has been in operation the piston seal will be destroyed and should also be repl...

Page 173: ...ater Ultrasonic bath Instruction Follow the instructions to remove and clean the pump head check valves Action Step Before taking the check valve apart always try to clean the check valves by priming...

Page 174: ...he upper check valve using an adjustable wrench and gently lift off the upper check valve 5 Unscrew the two white plastic screws located below each pump head Pull the plastic connectors to the sides t...

Page 175: ...for safe operation and mainte nance of the product 8 Immerse the valves completely in methanol and place them in an ultrasonic bath for a few minutes Repeat the ultrasonic bath with deionized water Re...

Page 176: ...ist in the On Site Service Health and Safety Declaration Form or the Health and Safety Declaration Form for Product Return or Servicing depending on whether the instrument is going to be serviced on s...

Page 177: ...25 Product Documentation and KTA avant 150 Product Documentation for detailed technical specifications In this chapter This chapter contains the following sections See page Section 178 8 1 System spe...

Page 178: ...ng inner diameter i d 1 6 mm Tubing connector 5 16 Ferrule yellow 1 8 Pump to Injection valve PEEK tubing i d 0 75 mm Fingertight connector 1 16 After Injection valve PEEK tubing i d 0 50 mm Fingertig...

Page 179: ...l resistance guide on page 180 Chemical environment 4 C to 35 C Operating temperature range 20 to 95 noncondensing Relative humidity Equipment noise level Acoustic noise level Equipment 70 dBA KTA ava...

Page 180: ...formation about chemical resistance of the KTA avant instrument In this section See page Section 181 8 2 1 General information about biocompatibility and chemical resis tance 183 8 2 2 Chemical resist...

Page 181: ...mn Valve V9 C The Column Valve V9 C contains a pressure sensor which can be damaged by 1 M hydrochloric acid Long time use of 0 2 M HCl connected to the Quaternary Valve Q9 as part of a BufferPro reci...

Page 182: ...nces are time and pressure dependent Unless otherwise stated all concentrations are 100 Note 182 KTA avant Operating Instructions 29101556 AB 8 Reference Information 8 2 Chemical resistance guide 8 2...

Page 183: ...that you have the MSDS available from your chemical distributor and or databases on the internet Note Aqueous buffers The specified aqueous buffers are suitable for continuous use CAS no EC no Concen...

Page 184: ...1 Solubilization and denaturing agents The following chemicals are suitable for continuous use as additives in separation and purification methods CAS no EC no Concentra tion Chemical 50 01 1 200 002...

Page 185: ...KTA avant User Manual for more information 2 Mobile phase system It is recommended to replace the mixer sealing ring with the highly resistant O ring product code 29 0113 26 if the system is to be ex...

Page 186: ...d EDTA 37482 11 4 253 523 3 20 mM Mercaptoethanol 7447 40 7 231 211 8 4 M Potassium chloride Other substances The following chemicals are suitable for continuous use CAS no EC no Concentra tion Chemic...

Page 187: ...t tubing for KTA avant 150 28980987 Tubing kit 10 1 5m FEP id 2 9 mm o d 3 17 Note Inlet tubing for KTA avant 150 28957217 Sample tubing kit for 7 inlets i d 0 75 mm Note Narrow inlet tubing for KTA a...

Page 188: ...e 18112707 Tubing connector for o d 1 16 tubing 18112117 Tubing connector for o d 1 8 tubing 18111249 Tubing connector for o d 3 16 tubing 11000339 Union 1 16 female to 1 16 female 5 pcs 11000852 Unio...

Page 189: ...d 10 to 21 mm 28956282 Column holder 28956270 Column holder rod 28956295 Flexible column holder 29011350 Loop holder 29011349 Multi purpose holder 29011352 Rail extension 28954329 Tube holder 5 pack...

Page 190: ...13 Piston kit 100 ml piston kit P9 S 28952640 P9 Piston kit 25 ml 18112866 Check valve kit check valves in out for P9 P9H and P9 S 28979368 P9H Piston kit 150 ml 28979373 P9H Seal kit 150 ml Mixer Cod...

Page 191: ...m 28956506 Column Valve V9 C 28956508 pH Valve V9 pH 28956510 Inlet Valve V9 IA 28962006 Inlet Valve V9 IB 28962007 Inlet Valve V9 IS 28956512 Outlet Valve V9 O 28956514 Injection Valve V9 Inj 2895722...

Page 192: ...e V9H IS 28979281 Outlet Valve V9H O 28979283 Injection Valve V9H Inj 28979303 Inlet Valve V9H A2 28979315 Inlet Valve V9H B2 28979320 Inlet Valve V9H S2 28979326 Inlet Valve V9H X1 28979328 Inlet Val...

Page 193: ...ing 150 ml 18112766 Fill port INV 907 18111089 Injection kit INV 907 28985812 Connector 1 16 Male Luer Female Built in fraction collector Code no Item 28954209 Cassette tray 28956402 Cassette for 50 m...

Page 194: ...e 95 10 18 mm 18112467 Tube Rack Complete 40 x 30 mm 18305103 Bowl 18305402 Tube Support 18646401 Tubing Holder 19724202 Tube Holder and Guide 175 12 mm 19868902 Tube Holder and Guide 95 10 18 mm 1811...

Page 195: ...ell 2 mm for U9 L 18112824 UV flow cell 5 mm for U9 L Conductivity monitors Code no Item 28956495 Conductivity Monitor C9 standard module 29011363 Conductivity monitor C9n optional External air sensor...

Page 196: ...External module cable short F type 29011366 External module cable long F type Flow restrictor Code no Item 18112135 Flow Restrictor FR 902 Barcode scanner Code no Item 28956452 Barcode Scanner 2 D wit...

Page 197: ...e tray 28956485 Front side waste tray User Documentation Code no Item 29035184 KTA avant User Manual Note Covers KTA avant 25 and KTA avant 150 KTA avant Operating Instructions 29101556 AB 197 8 Refer...

Page 198: ...testorothersuitablesurvey Adequate space andclearance is providedto allowsafe access forinstrumentservice repairor installation Insome cases this mayrequirecustomerto move equipmentfrom normaloperati...

Page 199: ...us orhazardous biological substances pleasespecify OtherHazardous Chemicals please specify Equipment must be decontaminatedpriortoservice return Pleaseprovide atelephone number whereGE cancontact youf...

Page 200: ...quirements on deep well plates 64 requirements on tubes 64 Fraction collector F9 C Cassettes and racks 121 G General precautions 18 H Holders ordering information 189 I Important user information 6 In...

Page 201: ...tions 178 Regulatory information 8 Requirements environment 58 power 60 Room requirements introduction 55 Run after run procedures 145 monitor a run 142 start 136 S Safety notices 7 Safety precautions...

Page 202: ...Software Products A copy of this Standard Software End User License Agreement is available on request 2015 2017 General Electric Company First published Mar 2015 All goods and services are sold subje...

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