17
6.7.
Calibration
Calibration can be performed to improve the accuracy of concentration measurements but is
not required for most proteins and peptides, as the degree of labelling is consistent for the
majority of samples. Calibration will not affect the size measurement as the rate of diffusion,
directly measured by the instrument, has a well defined physical relationship as described
by the Stokes-Einstein equation.
For outlying proteins which are particularly lysine rich or poor, we recommend performing a
one-point calibration run using a known concentration of your protein of interest. Full details
on how to perform a calibration can be found on our website
www.fluidic.com
Instrument priming
The instrument will automatically run a prime sequence to ensure that fluidic lines are fully
wetted whenever it is required. This will occur whether the instrument was turned on or off
during the idle period.
A prime sequence will run when there have been no tests run or the unit has been powered
down for 8 hours or more, or after a new cartridge has been connected.
The prime sequence will begin automatically when the first measurement run is started.
The prime takes around 10 or 20 minutes, depending on how much time has elapsed since
the last measurement run on the instrument.
The normal measurement run will begin straight after the prime sequence.
For the result given immediately after a prime, check the trace graph. If this looks normal,
the result can be used. If the trace is unusual in any way the result may have been affected
by the prime
–
in this instance repeat the test. See
www.fluidic.com
for tips on interpreting
traces.
Summary of Contents for Fluidity One
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