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FlowCam® 8000 Series Dynamic Imaging Particle Analyzer

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property rights except as expressly provided in any written license agreement from Fluid Imaging Technologies.

Fluid Imaging Technologies

FlowCam and VisualSpreadsheet are registered trademarks of Fluid Imaging Technologies, Inc. All other product

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Fluid Imaging Technologies

200 Enterprise Drive

Scarborough, Maine 04074 USA

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Summary of Contents for FlowCam 8000 series

Page 1: ...FlowCam 8000 Series Dynamic Imaging Particle Analyzer User Guide...

Page 2: ...marks copyrights or other intellectual or industrial property rights covering this document or subject matter in this document The furnishing of this document does not give a license to these property...

Page 3: ...Powering on Your Analyzer 12 Preparing the Analyzer 13 Checking Internal Connections 13 Adding Solutions 13 Installing the Flow Cell 14 Installing the Objective Lens 15 Autofocusing the Flow Cell 17...

Page 4: ...that you follow all operating instructions and that the analyzer s doors and housings remain closed while it is operating Operating the FlowCam 8400 analyzer with protective housing or doors that are...

Page 5: ...moving the instrument always carry it with both hands Grasp the front corner with one hand and place the other hand under the opposite side back corner for support Always use proper lifting techniques...

Page 6: ...analyzer uses dynamic imaging particle analysis to perform these key functions Examine a fluid under an objective lens backlit with a flashing LED light With a laser excite particles through a laser l...

Page 7: ...X 20X Lasers NA Excitation wavelengths Blue 488 nm 40 mW laser output Green 532 nm 40 mW laser output Red 633 nm 40 mW laser output Laser Detector Bands PMT Filters NA Emission wavelengths Blue Ch1 65...

Page 8: ...9 Analyzer Components Fluid position sensor Flow cell retainer thumbscrews Syringe valve Syringe pump manifold Upper hatch with safety interlock Tubing manifold Sample inlet port Lower door and bins f...

Page 9: ...aspirates the fluid sample through the flow cell which positions the particles within the optical system A high intensity LED light source combines synchronously with a very short shutter speed in th...

Page 10: ...ses on the center of the flow cell and when a particle with fluorescent properties passes through the beam the PMTs detect the fluorescent emission of light and trigger the optical system to capture a...

Page 11: ...ontact Fluid Imaging Technologies or your local distribution representative immediately Be sure to save the white shipping container that your FlowCam analyzer came in Note Use two people when lifting...

Page 12: ...o a power outlet 4 Turn on the monitor s power switch Powering on Your Analyzer 1 Connect the analyzer s power cord to the AC input port on the back of the analyzer and plug the other end into a power...

Page 13: ...cell apparatus Adding Solutions The FlowCam analyzer contains three fluid bottles one each for cleaner rinse and waste solutions The cleaner bottle should be filled with a cleaning solvent to remove...

Page 14: ...only labgrade water and low lint lens paper to clean the exterior of the flow cell Remove the flow cell from its protective case and inspect it for any damage Clean the outside of the flow cell with a...

Page 15: ...flow cell into its associated opening and fit the upper and lower tubing into their respective slots 6 Place the flow cell retainer into the slot so that the flow cell is held in place and tighten the...

Page 16: ...lens to move to the left so you can access the objective fitting 3 Carefully remove the objective lens from its container by unscrewing it from its cap 4 Gently thread the lens clockwise into the fitt...

Page 17: ...ofocus beads Low Magnification Focus Beads for the 300FOV or 600FOV flow cell approximately 50 m in diameter 60 000 p mL High Magnification Focus Beads or 25 m Focus Beads for the 80FOV flow cell appr...

Page 18: ...ired autofocus algorithm option Best Bead Count or Best Focus Image and click Next Note Select the algorithm depending upon whether you are running uniform materials for primarily counting or varied p...

Page 19: ...e manual focus process to adjust the focus of individual samples The autofocus log files are saved to Documents Visualspreadsheet Autofocus Logs Focusing the Flow Cell Manually To focus the flow cell...

Page 20: ...ign the red indicator lines as close as possible within the flow cell 3 Click the Manual Focus button A warning appears Click OK 4 Using a pipettor with an ejector pipette at least 0 5 mL of focusing...

Page 21: ...cles become clear The default value is 10 but you can increase or decrease the value to change the amount of objective movement Tip You can click the Pause Pump button to suspend the flow and achieve...

Page 22: ...he settings can be saved in a context file filename ctx within a folder that contains all the files for a particular analysis Basic software settings from the factory and your local default settings a...

Page 23: ...mages Saves all images segmented particles from a raw camera image captured during a run Save Binary Collage Images Saves a visual overlay of the pixels determined to be a particle Save Raw Camera Ima...

Page 24: ...ate efficiency run time priming type and sample dilution parameters Note The machine prime option uses the built in functionality of the fluid position sensor 8 The Filter tab lets you select an image...

Page 25: ...ename begins with the List filename appended with _notes txt 11 When an analysis finishes the Run Summary tab displays summary information about the sample analysis This data is saved as a text file i...

Page 26: ...ynamic Imaging Particle Analyzer 26 of 39 13 FlowCam 8400 analyzer only The Fluorescent Measurement Settings tab is where you set thresholds for particle acquisition and rejection 14 Click OK to save...

Page 27: ...into the sample introduction port on the top of the analyzer eject it and leave it there 2 Click the AutoImage or Trigger Mode tool on the toolbar The Stop Conditions dialog opens 3 Select any desire...

Page 28: ...the message and close the Pump Controller dialog Manual Clean and Rinse 1 Add some cleanser in a new tip in the analyzer s sample inlet port 2 Click the Setup and Focus tool and then click the Pump Co...

Page 29: ...ber of cycles to perform drop down list and select the desired number of rinse cycles 6 Close the Pump Controller dialog to save your settings Powering Down the Analyzer 1 Close the VisualSpreadsheet...

Page 30: ...The inspection should include removing the tubing from each end to look into the channel Carefully inspect your flow cell using a compound microscope at 40X total magnification especially the ends of...

Page 31: ...ndix A Changing the Pump Syringe The FlowCam analyzer has three pump syringes that accommodate the three objective lenses Objective Lens Power 4X 10X 20X Pump Syringe Size 5 mL 1 mL 0 5 mL 5 mL pump s...

Page 32: ...asks if you want to change the syringe Click Yes and follow the onscreen prompts 4 Turn the plunger lock screw counterclockwise three turns Click OK The plunger base moves down to allow for removal 5...

Page 33: ...39 8 The Select Syringe Size dialog appears Select the applicable size from the Syringe Size drop down list and click OK 9 Clean the previous syringe and replace it in its container Tip Remove the plu...

Page 34: ...jective the software retains the original position of that focus routine However you can override the autofocus routine by using the manual focus feature Q How often do I need to calibrate my analyzer...

Page 35: ...opriate for on boarding a new group of FlowCam analyzer users reviewing training topics for a new field season or training existing employees who need a refresher Remote learning sessions RLS are idea...

Page 36: ...field service report is completed and a copy of all documents are provided Laboratory Services We offer a full suite of FlowCam models for particle analysis and method development services customized...

Page 37: ...ny labor or other services related thereto and ii all shipping costs related to the repair or replacement of the Product or any defective Product components for both on site and off site repairs and r...

Page 38: ...of God The Seller will not be liable for any consequential indirect or incidental damages of any kind including lost revenues or profits in connection with the use of the Product The warranty period i...

Page 39: ...gies Inc of Scarborough Maine that the above referenced product to which this declaration relates is in conformity with the provisions of Council Directive 2004 22 EC December 15 2004 on Measuring Ins...

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