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SUPER

SESI

Airborne Molecules & Metabolites Analysis

User Manual

Revision 2.1 -February 2020

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Summary of Contents for SUPER SESI

Page 1: ...SUPER SESI Airborne Molecules Metabolites Analysis User Manual Revision 2 1 February 2020...

Page 2: ......

Page 3: ...this document is for reference purposes only The system configurations and specifications in this document supersede all previous information received by the purchaser This document is not a sales con...

Page 4: ...Page 2 of 34 SUPER SESI by FIT User manual Revision 2 0...

Page 5: ...ion cover 11 Components Nano amperemeter 11 Components nano amperemeter charging unit 12 Symbols used in the SUPER SESI 12 Technical specifications 13 Installation 14 Installation requirements 14 Unbo...

Page 6: ...e Series Orbitrap Exactive series LTQ Series LCQ Fleet Series Orbitrap LTQ Orbitrap Elite We are working to increase the number of compatible mass spectrometers If you have instrument compatibility qu...

Page 7: ...e make sure that you understand the special notices symbols and caution labels in this guide Most of the special notices and cautions appear in boxes those pertaining to safety also have corresponding...

Page 8: ...nded electrospray liquid is high purity water with 0 1 formic acid but other solvents can also be used including flammable solvents such as methanol or ethanol Use care when handling these solvents Al...

Page 9: ...ntify specific responses to controlled stimuli and to differentiate confounding variables from real discoveries SUPER SESI incorporates all the components and controls in one single unit It is ready t...

Page 10: ...Page 8 of 34 SUPER SESI by FIT User manual Revision 2 0 Complete system SUPER SESI Figure 1 SUPER SESI front view...

Page 11: ...User manual Revision 2 0 Components Main Body The main body incorporates the ionization chamber the sample line all the controls and the coupling with the mass spectrometer Figure 2 Main body front vi...

Page 12: ...corporates a positioning system that allows repeatable and optimum conditions It closes the ionization chamber and guides the sample gas smoothly to the electrospray tip to minimize convective evapora...

Page 13: ...gure 6 Sample inlet protection cover Components Nano amperemeter The nano amperemeter provides a measurement of the electrospray current This parameter and the current measured by the mass spectromete...

Page 14: ...ls used in the SUPER SESI Label Name Label design Location Purpose Identification label Besides power connector Identification Contact us Rear panel Contact info for technical assistance General warni...

Page 15: ...ample line temperature controller Identify controller Valve V1 identifier Near valve V1 Identify valve V1 Valve V2 identifier Near valve V2 Identify valve V2 Syringe inlet identifier 1 Near syringe in...

Page 16: ...ESI The SUPER SESI is packed with the nano amperemeter the vial holder the electrospray probe and the Mass Flow Controller in their working position In order to protect the SUPER SESI from contaminati...

Page 17: ...nstall the SUPER SESI on the mass spectrometer in the same way as the original Thermo ion source For this Carefully align the two guide pin holes on the rear of the SUPER SESI with the ion source hous...

Page 18: ...uid and the vial holder are free from contamination Always use gloves when manipulating them and make sure that all surfaces are very clean and free from any contamination that could affect your measu...

Page 19: ...wiping it with a Kimwipe soaked with new charging liquid Figure 13 Changing the electrospray liquid Clean the vial or use a new clean vial with new liquid The maximum level of liquid shall be 3 4 par...

Page 20: ...pray probe and the electrospray vial holder are free from contamination Always use gloves when manipulating them and make sure that all surfaces are very clean and free from any contamination that cou...

Page 21: ...hard materials Refer to the instructions provided with the silica capillaries for handling The new 20 m capillary has to be first inserted and locked in the electrospray probe For this first make sur...

Page 22: ...lary positioner align the reference sign of the capillary positioner and the electrospray probe handgrip and lock it in this position Then unlock the capillary lock and the Upchurch fitting 1 they sho...

Page 23: ...ch must be in the ON position Figure 19 SUPER SESI main switch IEC C14 connector and temperature controllers The SUPER SESI incorporates two independent Proportional Integral Derivative PID temperatur...

Page 24: ...temperature to be reduced before touching any surface after reducing the set value to safe temperatures CAUTION The temperature control settings are locked to make sure that the SUPER SESI is operated...

Page 25: ...a is measured 1000 times every second but refreshed more slowly on the display Press once the button above or below of any of the four flow parameters shown to highlight its value in the center of the...

Page 26: ...flows 2 Make sure that the setpoint at the MFC right up value is higher than the flow value read at the MFC center value to ensure that the clean gas is not restricted by the MFC 3 To fine tune the cl...

Page 27: ...sed to align the internal absolute pressure sensor with the barometric pressure reading When auto tare is ON your flow controller automatically tares its flow rate whenever it has a zero setpoint for...

Page 28: ...ulator Note that the high pressure circuit has some inertia You will see that the pressure display rises slowly when the regulator is actuated until it reaches a plateau Once the pressure is raised th...

Page 29: ...an the sample line between measurements To adjust the clean gas refer to section Adjusting the clean gas flow and the sample gas flow CAUTION Hot Surface At operating temperatures the sample line can...

Page 30: ...to remove this contamination it is advisable to rise the temperature from time to time so as to evaporate these contaminants and to sweep them away If the spray is on set the electrospray to zero See...

Page 31: ...as Kimwipes Cotton tipped applicators MeOH H2O HPLC grade and MeOH HPLC grade Tweezers and a tray Allen wrenches MS Syringe pump Cleaning the vapor path In the event that the SUPER SESI gets contamina...

Page 32: ...e soaked with the cleaning liquid CAUTION When cleaning the vapor path with cleaning liquid the SUPER SESI must not be connected to the mass spectrometer Solvents entering accidentally in the mass spe...

Page 33: ...lated and cannot be replaced When the batteries run out of charge you will need to recharge them Disconnect the Nano amperemeter from the SUPER SESI by pulling it gently and connect the nano amperemet...

Page 34: ...system cools down and restart the power If the problem persists contact FIT for technical assistance Replacing the fuses In order to change a fuse first make sure that the power cord is not connected...

Page 35: ...Fossil Ion Technology S L www fossiliontech com Calle la Gitanilla No 17 Nave 22 ZIP 29004 Malaga Spain email info fossiliontech com...

Page 36: ...SUPER SESI for Thermo User manual 2019...

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