Fisher Scientific 04-977-3000 Operation Manuals Download Page 2

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2000 Park Lane Pittsburgh, PA 15275
Tel. (800) 766-7000 Fax (800) 926-1166

MARATHON 3000(R)

General Purpose Centrifuge

Operation Manual

Model 3000 3L Ventilated Centrifuge

Cat. No. 04-977-3000 -- 120 VAC, 60 Hz
Cat. No. 04-977-3001 -- 220, 230, 240 VAC, 50/60 Hz

Model 3000R 3L Refrigerated Centrifuge

Cat. No.  04-977-3000R -- 120 VAC, 60 Hz
Cat. No.  04-977-30001R -- 220, 230, 240 VAC, 50 Hz
Cat. No.  04-977-30002R -- 230 VAC, 60 Hz

Part No. 04-977-3000M Rev. 4

Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com

Summary of Contents for 04-977-3000

Page 1: ...l service in house repair center WE BUY USED EQUIPMENT Sell your excess underutilized and idle used equipment We also offer credit for buy backs and trade ins www artisantg com WeBuyEquipment REMOTE INSPECTION Remotely inspect equipment before purchasing with our interactive website at www instraview com LOOKING FOR MORE INFORMATION Visit us on the web at www artisantg com for more information on ...

Page 2: ...Centrifuge Cat No 04 977 3000 120 VAC 60 Hz Cat No 04 977 3001 220 230 240 VAC 50 60 Hz Model 3000R 3L Refrigerated Centrifuge Cat No 04 977 3000R 120 VAC 60 Hz Cat No 04 977 30001R 220 230 240 VAC 50 Hz Cat No 04 977 30002R 230 VAC 60 Hz PartNo 04 977 3000M Rev 4 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 3: ... Recognition System 21 3 5 3000R Refrigeration 22 3 6 Diagnostic Messages and Error Codes 23 Section 4 Applications 4 1 Introduction 26 4 2 Speed and Force Tables 28 4 3 Derating Tables 31 4 4 Chemical Resistance Tables 33 4 5 Decontamination Table 34 4 6 RCF Nomograph 35 Section 5 Maintenance 5 1 Introduction 36 5 2 Care and Cleaning 36 5 3 Cover Interlock Bypass 39 5 4 Fuses Not Replaceable By O...

Page 5: ... can process a variety of tubes bottles and microsample tubes The 4 x 750 mL rotor can process both standard and deepwell microplates Also available are pear shape and short form conical oil tube rotors Each centrifuge has an easy to use front panel that provides two modes of operation Manual and Programmed Manual mode is used for entering temperature 3000R only speed force and time values for ind...

Page 6: ... and rigid construction provide quiet operation and long term reliability 1 2 About This Manual The Operations Manual contains all of the information needed to install operate and maintain a Marathon Series centrifuge Refrigerated and ventilated models operate similarly and any differences are highlighted and noted throughout this manual This manual also contains speed and force derating chemical ...

Page 7: ...t could result in personal injury A Caution advises against actions or situations that could damage equipment produce inaccurate data or invalidate a procedure A Note provides useful information regarding an operation function or procedure MARATHON Series Operation Manual 6 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 8: ...er than 4 C for processing temperature sensitive samples When you remove the centrifuge from a cold environment do not operate for a minimum of two hours so that any condensation will evaporate Note When used in a cold room environment some bearing noise may become evident The bearing lubricant thickens at low temperatures As the centrifuge speeds up it is thinned and distributed more evenly Once ...

Page 9: ...firmly Keep the area beneath the unit free of debris and loose materials The resting surface must be level to ensure quiet vibration free operation A rigid and stable location is important An improperly loaded centrifuge may vibrate or move Warning International Electrotechnical Commission standard 1010 part 2 20 limits the permitted movement of a laboratory centrifuge to 300 mm 12 in in the unlik...

Page 10: ...r supply 3 prong outlet If your facility does not have grounded power outlets arrange for a proper grounding The power cord plugs in on the left side of the unit Warning Do not remove the grounding pin from the centrifuge power cord Do not use the bare wired power cord to attach a power plug that does not have a grounding pin The power cord provided with the unit is correctly rated for the highest...

Page 11: ...can weigh up to 145 lb 65 8 kg Use precaution when moving to avoid any injury 1 Check that the new site meets the criteria in Section 2 2 before moving the unit 2 Position a flat object such as a tongue depressor near a suction cup at the bottom of the unit 3 Lift up an edge of the cup and insert the flat object far enough to break the vacuum seal 4 When all four cups are disengaged lift the unit ...

Page 12: ...me of or time remaining in the run actual temperature Refrigerated only program number In Set mode dim display the display indicates the desired settings for the run Set mode is activated when SPEED TIME TEMP or PROGRAM are pressed briefly at the start of a run briefly after the unit is switched ON The numeric displays can also display warning or error messages see Section 3 5 Descriptions of the ...

Page 13: ...nd then press ENTER Also see Section 3 4 Rotor Recognition System TIME HOLD SHORT SPIN Time key Pressing the TIME key switches the display from Actual to Set mode Select the desired time using the numeric keypad and then press ENTER A selection must begin before 5 seconds elapse or the display will revert to the Actual reading Time display The number in the display indicates time Time is displayed...

Page 14: ...operation see Section 3 2 Manual key The manual returns the unit to manual operation from Program mode Numeric Key Pad The numeric key pad is used to change the Set parameters for Speed Force Time Temperature Refrigerated only Rotor Radius or Program When any of the mode keys such as the SPEED TIME TEMP or PROGRAM key is pressed the numeric display switches from Actual readings to Set parameters w...

Page 15: ...rake Fast braking is selected when the indicator above the is lit Slow braking is selected when the indicator above the is lit When no indicators are lit coast no brake is selected The rotor will coast from operating speed to a stop START The Start key starts a run A run is governed by the Set parameters manual or programmed The green indicator light blinks until the rotor reaches 95 of the set ru...

Page 16: ...iers to within 1 gram and stamps the weight on each piece Check these markings whenever you interchange parts to ensure that opposite parts are matched Ensure that the total weight of samples and removable parts loaded in opposing positions are equal in weight to within 1 gram The position numbers present on many rotors and adapters identify opposing tube positions To obtain good dynamic balance o...

Page 17: ...achieve full swing out causes vibration and premature wear of the rotor and the motor Samples of like similar specific gravities may be processed in the same run provided that the samples of the same type are balanced around the rotor as though they were the only pairs in the rotor Caution Do not exceed maximum rated speed for each rotor accessory combination Maximum rated speeds can be found in S...

Page 18: ...n the locking nut Slide the wrench handle to one side in order to utilize maximum torque Remove the wrench Note There are two styles of locking nuts One is removable as shown in Figure 1 and one is captive not removable as shown in Figure 2 Wrench Captive Nut Removable Nut Recognition Ring Recognition Ring Figure 1 Removable Locking Nut Figure 2 Captive Locking Nut Rotor Removal To remove the roto...

Page 19: ...TART to start the spin The rotor will accelerate to 100 200 rpm and then coast for a few seconds while the rotor recognition system verifies the rotor and set parameters The time display counts down and displays the time remaining in the current spin during manual operation The specified run time begins when the START key is pressed Braking begins when the set time elapses Run time does not includ...

Page 20: ...ating that the program was changed and has not yet been saved Make the changes permanent by pressing the SAVE key The program number will stop flashing and the new program settings will be displayed The program will remain in memory until further changes are made Because the Marathon 3000 R has a fully automatic rotor recognition system the very first rotor that is spun using a particular program ...

Page 21: ... number on the numeric key pad and press ENTER The program s set parameters will be displayed Press START to begin this run Lock Program Programs can be locked by selecting the desired program on the numeric key pad and pressing the SAVE key three times When you scroll to a locked program the letter L will flash in the program display after the program number is displayed To unlock a program selec...

Page 22: ...onding message that will appear in the speed display window Condition Message Input Speed value exceeds maximum allowable SPD toggled with the max allowable speed setting for the rotor Input RCF value exceeds maximum allowable rCF toggled with the max allowable RCF setting for the rotor Input Radius value exceeds maximum allowable RAD toggled with the max allowable radius setting for the rotor No ...

Page 23: ... C display will switch between the actual and set programmed temperatures until the two temperatures come within 5 C Press the STOP key if the run should not continue at the actual temperature The unit is not designed for use as a refrigerator The natural fanning action of the rotor serves to maintain a uniform temperature distribution inside the chamber At zero RPM there is no correlation between...

Page 24: ... failure The front panel will alternate between the PFAIL message and the remaining run time or elapsed time if in Hold mode Press STOP to erase this message Press START to resume the previous run RAD RAD appears and flashes with the max allowable value if the input radius is greater than the maximum allowable for the rotor detected Press the STOP key to clear this message and when in PROGRAM mode...

Page 25: ...as been detected a short to ground has occurred in the motor The rotor will coast to a stop To clear the error unplug the unit or turn power switch off and reconnect the unit or turn power switch on Check for obstruction of the cooling fans or vents If repeated dFL errors occur contact service personnel MARATHON Series Operation Manual 24 Artisan Technology Group Quality Instrumentation Guaranteed...

Page 26: ...5 C at any time during the run norEF No Refrigeration Temp Sensor Refrigerated only The unit displays this code if the microprocessor is not detecting a temperature measurement from the temperature sensor FSAFE Fail safe Time out Independent circuitry on the circuit board has sensed a lack of activity from the control microprocessor All power circuits including motor latch solenoid etc are disable...

Page 27: ...nd Force Tables Relative Centrifugal Force RCF or G force at a given speed varies with the rotor and with the distance away rotating radius from the shaft of the centrifuge center of rotation The rotating radius is measured to the furthest inside tip of the tube away from the centrifuge shaft The Speed and Force Tables indicate the maximum speed and RCF that the 3000 3000R can achieve with various...

Page 28: ...posure should be avoided by immediately removing the chemical from rotor or assembly Rotors and accessories placed in the chamber are made of a variety of materials including aluminum and polypropylene The Chemical Resistance Table shows the suitability of each material with different classes of reagents Section 5 2 describes how to clean and remove corrosion from the chamber rotors and accessorie...

Page 29: ...440 18 9 4 x 04 974 008G 48 x 10 15ml sealed Vacutainer 16x100 16x125mm 3400 2520 19 5 4 x 04 974 008L 60 x 7 10ml sealed Vacutainer 16x75 100mm 3400 2260 17 5 4 x 04 974 008H 100 x 7ml sealed Vacutainer 13x100mm 3400 2220 17 2 4 x 04 974 008I 100 x 7ml sealed Hemogard Vacutainer 13x100mm 3400 2220 17 2 4 x 04 974 008I 100 x 5ml sealed Vacutainer 13x75mm 3400 2220 17 2 4 x 04 974 008I 100 x 5ml se...

Page 30: ...er 13x100mm 3600 3000 20 7 6 x 04 974 007C 54 x 7ml sealed Vacutainer 16x75mm 3600 2620 18 1 6 x 04 974 007D 54 x 5ml sealed Vacutainer 13x75mm 3600 2620 18 1 6 x 04 974 007D 54 x 5ml sealed Hemogard Vacutainer 13x75mm 3600 2620 18 1 6 x 04 974 007D 72 x 5ml sealed Hemogard Vacutainer 13x75mm 3600 2430 16 8 6 x 04 974 007E 72 x 3 5ml sealed 10x75 12x 75mm Falcon 2063 3600 2430 16 8 6 x 04 974 007E...

Page 31: ...ion and 1 mounted rotor recognition disk Tube Maximum No x Vol ml Description RPM RCF Radius 4 x 100ml 05 621 short form conical tube 2450 1350 20 1 Microplate Rotor Assy 04 974 011 4 Place Swinging Bucket 4xMicroplate Complete with 4 removable 5782 buckets and 1 mounted rotor recognition ring No of Places Tube Volume ml Adapter Number Maximum Speed rpm Max RCF xg Max Radius cm Max Tube Size O D x...

Page 32: ...ated by a factor from the table below Derating Factor for Specific Gravity Swinging Bucket 1 2 1 000 1 3 960 1 4 925 1 5 894 1 6 866 1 7 839 1 8 816 1 9 794 2 0 774 2 1 755 2 2 738 2 3 721 2 4 707 2 5 692 2 6 678 2 7 666 2 8 654 2 9 642 3 0 632 Derating Example A rotor rated for 4 000 RPM used with samples with a specific gravity of 1 4 cannot spin faster than 3 700 RPM 4 000 x 925 3 700 MARATHON ...

Page 33: ... derating 1 2 Sa is the actual specific gravity of the sample Caution Do not exceed the rated speed or specific gravity Higher speeds or specific gravities will impose unnecessary wear on the centrifuge and can cause rotor failure Wear and damage caused in this manner are not covered under warranty MARATHON Series Operation Manual 32 Artisan Technology Group Quality Instrumentation Guaranteed 888 ...

Page 34: ... G G G G G G F N E E E E E E N E E Bases E N E E N G N G F E E E E E E G G N N Esters G N G G N E G G E N E E E E E N N N E Hydrocarbons aliphatic G F G G E N E E E N E E E E E N E E E Hydrocarbons aromatic F N G F N N E E E N E E E E E N N E E Hydrocarbons halogenated F N F F N N G E G N E E E E N N N F E Ketones G N G G N N E E E N E G G G E N N N E Oxidizing Agents strong F N F F N N N N N N E ...

Page 35: ... S S U 5 Phenol Solution M U U S U U M M U M M M M M M M U S S U For Oxidizing Acids see Oxidizing Agents strong PA POLYALLOMER TI TITANIUM Autoclaving PC POLYCARBONATE SS STAINLESS STEEL 121 C for 20 min PE POLYETHYLENE AL ALUMINUM 2 ATM 15 PSIG PP POLYPROPYLENE MB MANGANESE BRONZE 1 to 10 Dilution of PU POLYURETHANE MG MAGNESIUM Household Bleach NL MODIFIED PHENYLENE OXIDE NORYL RR RUBBER DN ACE...

Page 36: ...4 6 RCF Nomograph MARATHON Series Operation Manual 35 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 37: ...rotor and lid at the end of each work day and immediately after any spill To clean the chamber use a damp sponge warm water and a mild liquid detergent suitable for washing dishes by hand such as Ivory liquid Do not use caustic detergents or detergents that contain chlorine ions These attack metals Remove stubborn stains with a plastic scrub pad Do not use steel wool wire brushes abrasives or sand...

Page 38: ...aline and sodium hypochlorite household bleach because these solutions attack most metals Clean the rotor rotor chamber and accessories particularly the sample compartments and bucket cups thoroughly after each exposure Inspect all surfaces under bright light for corrosion Be aware that small crevices grow deeper eventually resulting in system failure If you see any corrosion remove it immediately...

Page 39: ...ontaminate metal rotors or accessories follow decontamination by the corrosion cleaning procedure See 5 2 since chloride ions attack most metals Always decontaminate for the minimum recommended time If you observe corrosion remove it as described earlier discontinue use of the method and use an alternate decontamination procedure Polypropylene sealed carriers can be autoclaved Remove any sample tu...

Page 40: ...o release the cover interlock 5 Replace the plug in the hole Do not perform this operation routinely The centrifuge s cover interlock provides operator safety It allows the cover to be opened promptly whenever rotation has stopped 5 4 Fuses not replaceable by operator The following fuses are located internally within the centrifuge on the power PC board They should only be replaced by qualified se...

Page 41: ...defects in material and workmanship and we will ship you the repaired product or its replacement at our expense You must use Fisher Scientific approved rotors and accessories and genuine Fisher Scientific spare parts This warranty does not apply to any instrument that has been abused or repaired without authorization THE FOREGOING OBLIGATIONS ARE IN LIEU OF ALL OTHER OBLIGATIONS AND LIABILITIES IN...

Page 42: ...RCF 50 3 000xg by 50 xg Spin Duration 0 99 min 59 sec by 1 min and or 1 sec HOLD MODE Momentary Spin Brake Dynamic Electric 3 profiles Repeatability Temperature Control 3000R only 2 C within range of 4 C to ambient Rotation Accuracy within 10 RPM Timer Microprocessor controlled Accuracy within 1 sec System Components Motor 3 8 hp Brushless DC Refrigeration System 3000R only 1 2 hp nominal MARATHON...

Page 43: ...20 240 VAC 50 Hz 6463 3000R Refrigerated 230 VAC 60 Hz Current 15 Amps Heat Output 3000 1860 Btu hr 3000R 3600 Btu hr Dimensions Height 3000 and 3000R 16 5 in 41 9 cm Width 3000 23 25 in 59 1 cm 3000R 30 0 in 76 2 cm Depth 3000 and 3000R 25 75 in 65 4 cm Shipping Dimensions Height 27 in 68 6 cm Width 36 in 91 4 cm Depth 30 in 76 2 cm Unit Weight 3000 118 lb 53 5 kg 3000R 145 lb 65 8 kg Shipping We...

Page 44: ...l service in house repair center WE BUY USED EQUIPMENT Sell your excess underutilized and idle used equipment We also offer credit for buy backs and trade ins www artisantg com WeBuyEquipment REMOTE INSPECTION Remotely inspect equipment before purchasing with our interactive website at www instraview com LOOKING FOR MORE INFORMATION Visit us on the web at www artisantg com for more information on ...

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