2. Microscope Alignments
1.
Eucentric height adjustment
(a) Open the column valves under the
Setup
menu. At a magnification of about 4000-
5000 x, find a noticeable feature on your specimen.
(b) Activate the alpha-wobbler.
Change the
Z
height on the right-hand control panel to minimize the image
displacement.
(c) Stop the wobbler. Press on “Eucentric Focus” on the right-hand control panel.
2.
Centering the condenser aperture. Condenser:
(a) Select a C2 aperture (100um for TEM/Single particle)
(b) At a magnification of ~10,000, go to crossover and center the beam using
the left trackball.
(c) Expand the beam and make sure its expands concentrically. If not center it using the
condenser aperture position knobs.
3.
Condenser lens stigmation:
Go to a magnification of 135,000X and go to crossover.
Under the
Tune
menu click go to
Stigmator
and click on
Condenser
. Use
multifunction knobs X and Y to obtain a triangular crossover beam. Once done click
on
None
to exit.
