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3.7.2.3 Method Definition: Samples
Samples can be placed either individually by moving the cursor to the desired location. Alternatively all
wells not already used can be filled with samples. Replicate samples can be used in combination with
single samples when desired. There is no limit on the replicate number.
1.
Options
Press F3 to define default directions (horizontal or vertical)
for replicates of samples and for different samples. Move
the
symbol to the desired option with the up / down
arrow keys and select the option by pressing either the left
or right arrow key. Press F4 to accept the entries.
2.
Place samples
Place the first sample (SM1) and replicates using F4. Press
F1 to get SM2. Use F4 to place the second sample and
repeat as necessary. Replicates of samples can be placed in
any free location; it is not necessary that the wells are
adjacent.
3.
Fill all
To reproduce the first sample pattern for all samples:
Sample 1 has to be defined. The Function ‘Fill All’ (F2) will
fill the plate accordingly as set in ‘Options’.
4.
To erase all already defined sample positions press the Backspace (
) for a few seconds.
5.
Press enter to move to the next method definition box.
3.7.2.4 Method Definition: Standards
In case standards have been defined in the layout section, the calibration screen appears.
Use this option to enter the values of the standards in addition to the units for the concentration and to
choose the curve-fit method (model).