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SECTION 7
SOLVING PROBLEMS
7.1 Troubleshooting
Table 13: General Troubleshooting and Diagnosis (continued)
Problem
Solution
Abnormal staining on entire surface of all slides
(continued).
When staining a full carousel (7 or more slides) make
certain you have not programmed the stainer for fewer
slides.
If staining a partial load, load the slides in the correct
positions as indicated by the markings on the carousel
(see Section 4.1).
Abnormal staining on entire surface of some slides, while
other slides from the same carousel appear normal.
Make certain that all position magnets are still attached
to the bottom of the carousel. Make certain you have not
programmed the stainer for fewer slides than you have
loaded.
If you program the stainer for a partial load, load the
slides in the correct positions as indicated by the
markings on the carousel (see Section 4.1).
Verify that each reagent pump is primed by opening the
lid and pressing the corresponding prime button. The
nozzle should immediately spray a fine mist of reagent.
There should be no sputtering or hissing sounds to
indicate the presence of air in the reagent lines (see
Section 7.3).
Streaks or bands of discoloration on one or more slides.
Check the level of the Rinse (Reagent A) bottle. Check
Rinse (Reagent A) spray volume according to Section 6.4.
Check nozzle spray pattern according to the procedures
in Sections 4.1 and 6.5. This type of discoloration is
usually caused by debris or reagent precipitate clogging
the spray nozzle orifice.
Clean any nozzle that exhibits a poor spray pattern.
Cells are washing off slides.
Check methanol or Aerofix (Reagent D) level in bottle.
Check Reagent D nozzle spray volume and slide pattern
(see Section 6.4 and Section 6.5).