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elements

enabling technologies for Life Science

eONE - User’s guide

SECTION

3

3

Software installation and description

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Software guide.



Requirements.

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Installation.



Software Operation Guide.

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Graphical User Interface.



eONE Controls.



Digital Offset Compensation.



Statistic Data.



Trace Visualization.



Save Data.

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Summary of Contents for eONE

Page 1: ...eONE Miniaturized One Channel Amplifier USER S GUIDE October 2015 elements enabling technologies for Life Science...

Page 2: ...d troubleshooting guide 29 th September 2014 Version 1 3 Edited Corrected pages headings and Summary 10 th October 2014 Version 1 4 Added the note in section 2 4 6 and suggestion in the troubleshootin...

Page 3: ...n and connections 7 2 1 Product description 8 2 2 Key features 8 2 3 Package 9 2 4 Hardware setup 10 2 4 1 Front Connections 10 2 4 2 Rear Connections 11 2 4 3 Select the location 12 2 4 4 Checking th...

Page 4: ......

Page 5: ...ion General notes Trademarks Instructions on disposal Description of safety symbols Qualified Personnel Basic knowledge required Proper use of the product Purpose of the user s guide Scope of the oper...

Page 6: ...smaltimento rifiuti o il fornitore da cui stato acquista to il prodotto The symbol on the product or in its packaging indicates that this product may not be treated as household waste Instead it shall...

Page 7: ...producto no se man pula de forma adecuada Para obtener informaci nes mas detalladas sobre el reciclaje de este producto p ngase en con tacto con la adMinistraci n de su ciudad con su servicio de dese...

Page 8: ...used for the applications described in the catalogue and in the technical documentation b If products and components from other manufacturers are used these must be approved by ELEMENTS S R L c Prope...

Page 9: ...he eONE family 1 10 The manual is a part of the system b This user s guide belongs to eONE and is also required for commission ing c Keep all supplied documentation for the entire service life of eONE...

Page 10: ...system in which the eONE device is to be installed complies with all the applicable EU and international regulation 1 13 Notes about usage eONE is approved for indoor use only eONE may be damaged if o...

Page 11: ...ION 2 2 General description and connections Product description Key features Package Hardware Setup Front Connectors Rear Connectors Preparation for installation Select the location Checking the packa...

Page 12: ...tem entirely contained in a USB stick thanks to a custom made microchip for low noise current signal acquisitions 2 2 Key features Two input current ranges 200 pA and 20 nA Eight selectable sampling r...

Page 13: ...9 elements enabling technologies for Life Science eONE User s guide Table 2 Package 2 3 Package eONE package consists of eONE Software interface SMB plug adaptor USB cable Ground cable...

Page 14: ...be careful on are parasitic capacitances on the input and correct shielding of the overall hard ware setup to avoid the 50 60Hz line interferences The following paragraphs describe all the connections...

Page 15: ...ear Connections 2 4 2 Rear Connections USB LED Ground On the rear of eONE there are two connectors mini B female USB connector to connect to the PC through the in cluded USB cable 2mm gold plated grou...

Page 16: ...essible to the op erator c Choose a suitable mounting height 2 4 4 Checking the package contents Check the package content for visible signs of transport damage and for completeness In the case of dam...

Page 17: ...ith a metallic cage Faraday cage all around the experimental setup In particular it is important to connect the Faraday cage to the ground con nector placed on the rear of eONE In order to minimize th...

Page 18: ...14 elements enabling technologies for Life Science SECTION 2 General description and connections...

Page 19: ...ce eONE User s guide SECTION 3 3 Software installation and description Software guide Requirements Installation Software Operation Guide Graphical User Interface eONE Controls Digital Offset Compensat...

Page 20: ...i tion properties and the control voltage and enables the real time data visualiza tion and data saving Data acquired by means of this custom software are handily readable by Matlab and can be saved t...

Page 21: ...e Figure 7 FTDIChip CDM Drivers Figure 8 Fevice Driver Installation Wizard During the installation process you will be asked to install the FTDI driver needed to communicate through USB to the eONE de...

Page 22: ...tallation er ror unplug eONE from the USB and plug in again Then start the FTDI installation by clicking on Next button and wait until installation finishes Now the Elements Data Reader software is co...

Page 23: ...e first operation is to load a configuration file to initialize the software for the eONE devices The configuration file is loaded by clicking on the Open Config button The configuration file is eONE...

Page 24: ...nnel available See the Trace Visualization section for details On the right of the main menu there is also an estimation of the input bitrate In the bottom you can find in addition to the Open Config...

Page 25: ...led or disabled depending on the eONE version Below is described the HS version control tab which include all the possible features The Controls tab contains the following controls Range Sampling Rate...

Page 26: ...plication of the selected stimulus proto col using the pre set parameters The Clear button cancels all the parameters and set them to the default values The Save button enables to save all the paramet...

Page 27: ...otocol The constant voltage is considered as a protocol with only the Vhold parame ter Triangular wave protocol It can be used to control the bilayer formation and it is used for the Capaci tance esti...

Page 28: ...p mV The time interval between two consecutive pulses interpulse interval changes of the value of the Tstep parameter It can be positive or negative to obtain increasing or decreasing interpulse inter...

Page 29: ...x Vhold tab The Triangular Wave control is used to force a 50 Hz triangular wave control volt age of peak to peak amplitude of 200 mVpp This voltage signal is commonly used to control lipid bilayer fo...

Page 30: ...l calibration procedure that can be acti vated with the Digital Compensation check box The compensation circuit when activated sets the Vc to 0 V and adds to it a vari able voltage in the range from 1...

Page 31: ...it some time to have valid estimated statistic data The following instructions have to be followed to obtain capacitance and re sistance estimations Capacitance estimation Enable the Triangular Wave u...

Page 32: ...essary to control the visualiza tion In particular from left to right these are the button functions Increases decreases verti cal zoom NB click and drag in the window to zoom only the interested part...

Page 33: ...lay current offset Shift Increase Decrease display voltage scale Shift Page Up Down Increase Decrease display voltage offset Ctrl R Reset scales Ctrl Arrow Up Down Increase Decrease Vhold by 10 mV Ctr...

Page 34: ...irectory usually C Program Files x86 Elements Data Reader and in the folder where data are saved Data samples are stored in 32 bit single precision format little endian The elementsRead Matlab functio...

Page 35: ...str Nch end look for range line if strfind line Range 0 unused remainder strtok line secondToken strtok remainder switch str2double secondToken case 200 range 0 display 200 pA range case 20 range 1 di...

Page 36: ...10 OSR 128 case 5 OSR 256 case 1 25 OSR 1024 end end end if OSR 0 OSR 64 display Warning cannot read sampling rate from header file using default 20 kHz end fclose fid close file else select number o...

Page 37: ...olumns Nch channels vc C reshape B 1 Nch 1 fix length B Nch 1 Nch 1 D C display final bandwidth display sprintf Sampling rate 0f Hz sampl OSR display sprintf Bandwidth 0f Hz PF sampl OSR return arrays...

Page 38: ...and then set Comma as delimiter in the Import Option menu The first imported column is the current signal in pA or nA while the second column is the control voltage in mV To read data using Clampfit...

Page 39: ...lectrodes are im mersed in the solu tion Electrode potential or wrong connections Compensate elec trode potential using the built in Digital Compensation see section 3 1 6 and check input and back con...

Page 40: ...chnical support The staff who deal with this handle questions on the entire range of products skillfully quickly and efficiently You can phone our staff in the service department and they will give yo...

Page 41: ...gular Voltage Pulses protocol 22 Figure 14 eONE Controls Voltage Protocols Constant Voltage protocol 23 Figure 15 eONE Controls Voltage Protocols Triangular wave protocol 23 Figure 16 eONE Controls Vo...

Page 42: ...Elements s r l via Montalti 42 47521 Cesena ITALY email info elements ic com web www elements ic com elements enabling technologies for Life Science...

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