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THE MUNRO AW400TG ANAEROBIC WORKSTATION SPECIAL FEATURES 

 

 

Many special  features  have  been  designed  into  the  MUNRO Anaerobic  Workstation  that cannot be 

found on any  other  cabinet. All these have been built in for safety, economy of operation and to make it 

easier and more comfortable for the operator to use. 

 

 

MINIMUM BENCH AREA 

 

 

Although the cabinet will incubate 400 dishes there is still ample room within the unit for the operator to 

manipulate the cultures. 

 

 

OPERATOR COMFORT 

 

 

Quite often the operator has to spend long periods of time working with their arms inside the unit and for 

this reason the cabinet has been designed so that the operator can work sitting down thereby minimising 

fatigue. The openings of the glove ports are large, facilitating easy movement of the arms. The viewing 

area is large, unobstructed by bends in the material which cause the operator to be constantly moving 

their position. The doors are easily sealed by first closing, then turning the tightening knob. If the unit fails 

to function because one of the doors has been left open or perhaps a gas bottle has run out of gas then 

this is indicated clearly on the Control Panel. The unique bare hand working facility allows the operator to 

get their hands in and out of the cabinet without any fuss.  In fact the cabinet has been designed to be 

very user friendly. 

 

 

 

SINGLE PLATE ENTRY LOCK 

 

 

This lock is used when only a small number of dishes need placing in the incubator. It saves both time and 

gas. The front door is opened by unscrewing the hinge pin and the dish is placed in the cabinet by pushing 

it with the bar provided. The dish lid travels up a ramp allowing the escaping gas from the cabinet to flush 

the air out of the dish before the lid is replaced by the back door. Once the dish is in the incubator the 

outer door is re-closed by tightening the screw. 

 

 

 

CHECKING THE INCUBATOR ATMOSPHERE FOR OXYGEN 

 

 

The Anaerobic Indicator Solution will indicate traces of Oxygen in the incubator atmosphere by changing 

colour from being clear when the atmosphere is anaerobic to being blue when oxygen is present. The 

Indicator Solution is held in a small bottle located at the front of the incubator in the top right hand corner 

and held in position by a plastic spring clip. The atmosphere from the cabinet is bubbled through the 

solution by a small aquatic pump. The pump is plugged permanently into an electrical socket and the 

switch should be left in the "ON" position. The atmosphere of the incubator can be checked for oxygen 

content by simply switching the socket "ON" on the Control Panel. It is usually sufficient to check the 

atmosphere perhaps once a day allowing the pump to run 

for about 1 minute. 

 

Summary of Contents for AW400TG TWO GAS

Page 1: ...ANAEROBIC WORKSTATION AW400TG TWO GAS VERSION MANUAL 44 45 Burnt Mill Elizabeth Way Harlow Essex CM20 2HU United Kingdom Telephone 44 0 20 8551 7000 Website www munroinstruments com...

Page 2: ...G THE GAS FLOW 11 13 TRANSFERRING ITEMS INTO THE INCUBATOR 12 14 TRANSFERRING ITEMS OUT OF THE INCUBATOR 12 15 SINGLE PLATE ENTRY 12 16 REMOVAL OF CONDENSATION FROM INSIDE THE INCUBATOR 13 17 THE GAS...

Page 3: ...lushing interlock 60 Petri dish capacity Condensation Plate Cooling air inlet Manually sealed interlock doors Control panel Anaerobic indicator bottle Single plate entry Omitted Clear acrylic window 5...

Page 4: ...Petri Dishes DOOR SEALING Manual GASES 1 Mixed Gas 10 H2 10 CO2 80 N2 2 Oxygen free Nitrogen N2 OXYGEN REMOVAL FROM AIRLOCK Positive Pressure flushing with catalyst FABRICATION MATERIAL Acrylic OVERA...

Page 5: ...re as follows MIXED GAS 25L min N2 25L min The diameter of the pipes connecting the cylinders to the cabinet should be selected according to the length of run to give a minimum pressure drop this will...

Page 6: ...rimary gauge showing pressure in bottle Regulator Gas isolation taps close to cabinet 6mm diameter flexible plastic tube 1m long Electrical supply 220 240V AC 50Hz Mount cabinet on strong bench at con...

Page 7: ...tor and fit collection pipe from cool plate Conditioner Catalyst Hang indicator pump here and plug into adjacent socket Clip anaerobic indicator bottle here Place conditioning sachet and palladium cat...

Page 8: ...to gauntlets Gauntlet holder Hang gauntlet on Gauntlet Hooks with Gauntlet Holders when not in use Fix gauntlet assembly to Arm Ports with O rings ensure there are no creases in the gauntlets undernea...

Page 9: ...d out of the cabinet without any fuss In fact the cabinet has been designed to be very user friendly SINGLE PLATE ENTRY LOCK This lock is used when only a small number of dishes need placing in the in...

Page 10: ...the bottle and place it in a bath of water and bring it to the boil The solution will change from being blue to colourless Tighten up the cap and remove the bottle from the water allow it to cool and...

Page 11: ...e cabinet Place the small Palladium Catalyst in the roof of the Lock See Sketch on Page 17 6 Now switch on the cabinet at the ELCB If either of the Low Gas Pressure neons is illuminated increase the a...

Page 12: ...Pressure Gauge 0 3 3 Bar Connect up the equipment as shown and start the Lock Flush Disconnect the gas supply to the cabinet at point X and insert a flow meter The meter should vent to the atmosphere...

Page 13: ...the Lock Anaerobic Indicator on the Control Panel will become illuminated At this point place the hands inside the incubator using the glove ports see Working inside the cabinet with bare hands Page 1...

Page 14: ...nsation Plate and has several functions Firstly it is a safety device so that in the event of a gas Input Solenoid remaining open the gas has a way of getting out of the cabinet and not doing any dama...

Page 15: ...warmer gas is now forced down the channel at the back of the cabinet and passes under the bottom shelf of the incubator where it first meets the sachets of Conditioner and then the sachets of Catalyst...

Page 16: ...ove the hands from inside the cabinet simply collect the Bung and release it within the sleeve Remove the hand and let the Bung fall into the Cuff where it will seal Gravity will now hold the Bung in...

Page 17: ...y Description Recommended Replacement 2 Gauntlets 1 year 2 Cuffs 6 months 3 Fans 2 years 2 Heaters 3 years 1 Outer Door Seal 6 months 1 Inner Door Seal 6 months 1 Single Port Door Seal 6 months 2 Larg...

Page 18: ...et of catalyst housed in the roof of the lock This sachet can be both inserted and removed by bending it slightly and sliding it in or out of the holder CHANGING THE FLUORESCENT LIGHTING TUBE Switch o...

Page 19: ...E 30 PSI MIXED GAS FLOW 25 L MIN 33L LOCK FLUSH N2 GAS FLOW 25 L MIN 50L LOCK FLUSH MIXED GAS INPUT PIPE SIZE 6 mm DIA N2 GAS INPUT PIPE SIZE 6 mm DIA INCUBATOR POSITIVE PRESSURE SETTING 6 mm WG INCUB...

Page 20: ...44 45 Burnt Mill Elizabeth Way Harlow Essex CM20 2HU UK Description of Equipment Incubator for the environmentally controlled incubation of anaerobic bacteria I certify that the apparatus identified a...

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