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17 

CHANGING THE CATALYST AND CONDITIONER 

 

 

The sachet of Catalyst and the sachet of Conditioner which are housed beneath the bottom shelf of the 

incubator will require replacing at a period in excess of one year. If for any reason the sachet of catalyst   

gets wet, the contents can be dried out by heating to a temperature of 160

°

C for a period of two hours. 

The sachet of conditioner can, if necessary, be dried at room temperature. The chemicals can then be 

returned to their respective containers for further use.  

 

When replacing the sachets, ensure that the sachets of Atmospheric Conditioner are at the REAR of the 

cabinet so that the gas passes over them first. (See sketch below) 

 

 

 

There is also a sachet of catalyst housed in the roof of the lock. This sachet can be both inserted and 

removed by bending it slightly and sliding it in or out of the holder. 

 

 

 

CHANGING THE FLUORESCENT LIGHTING TUBE 

 

 

Switch off the power to the light on the Control Panel and remove the three screws from the top and the 

two plastic screws from the front of the Light Shade. The tube can now be removed from its Holding Clips, 

the end caps disconnected and the new tube fitted. 

 

 

CLEANING 

 

 

The inside of the cabinet can be swabbed out with a weak solution of general laboratory detergent and 

warm water, and dried with a soft cloth. In the event of a culture spillage, a 5% Hypochlorite solution 

should be applied to the area and left for half an hour. It should then be wiped and swabbed with a 1% 

solution of Sodium Thiosulphate. Remove the catalysts and conditioners from the unit while the 

Hypochlorite solution is being used. 

 

 

 

 

 

 

 

Summary of Contents for AW400TG TWO GAS

Page 1: ...ANAEROBIC WORKSTATION AW400TG TWO GAS VERSION MANUAL 44 45 Burnt Mill Elizabeth Way Harlow Essex CM20 2HU United Kingdom Telephone 44 0 20 8551 7000 Website www munroinstruments com...

Page 2: ...G THE GAS FLOW 11 13 TRANSFERRING ITEMS INTO THE INCUBATOR 12 14 TRANSFERRING ITEMS OUT OF THE INCUBATOR 12 15 SINGLE PLATE ENTRY 12 16 REMOVAL OF CONDENSATION FROM INSIDE THE INCUBATOR 13 17 THE GAS...

Page 3: ...lushing interlock 60 Petri dish capacity Condensation Plate Cooling air inlet Manually sealed interlock doors Control panel Anaerobic indicator bottle Single plate entry Omitted Clear acrylic window 5...

Page 4: ...Petri Dishes DOOR SEALING Manual GASES 1 Mixed Gas 10 H2 10 CO2 80 N2 2 Oxygen free Nitrogen N2 OXYGEN REMOVAL FROM AIRLOCK Positive Pressure flushing with catalyst FABRICATION MATERIAL Acrylic OVERA...

Page 5: ...re as follows MIXED GAS 25L min N2 25L min The diameter of the pipes connecting the cylinders to the cabinet should be selected according to the length of run to give a minimum pressure drop this will...

Page 6: ...rimary gauge showing pressure in bottle Regulator Gas isolation taps close to cabinet 6mm diameter flexible plastic tube 1m long Electrical supply 220 240V AC 50Hz Mount cabinet on strong bench at con...

Page 7: ...tor and fit collection pipe from cool plate Conditioner Catalyst Hang indicator pump here and plug into adjacent socket Clip anaerobic indicator bottle here Place conditioning sachet and palladium cat...

Page 8: ...to gauntlets Gauntlet holder Hang gauntlet on Gauntlet Hooks with Gauntlet Holders when not in use Fix gauntlet assembly to Arm Ports with O rings ensure there are no creases in the gauntlets undernea...

Page 9: ...d out of the cabinet without any fuss In fact the cabinet has been designed to be very user friendly SINGLE PLATE ENTRY LOCK This lock is used when only a small number of dishes need placing in the in...

Page 10: ...the bottle and place it in a bath of water and bring it to the boil The solution will change from being blue to colourless Tighten up the cap and remove the bottle from the water allow it to cool and...

Page 11: ...e cabinet Place the small Palladium Catalyst in the roof of the Lock See Sketch on Page 17 6 Now switch on the cabinet at the ELCB If either of the Low Gas Pressure neons is illuminated increase the a...

Page 12: ...Pressure Gauge 0 3 3 Bar Connect up the equipment as shown and start the Lock Flush Disconnect the gas supply to the cabinet at point X and insert a flow meter The meter should vent to the atmosphere...

Page 13: ...the Lock Anaerobic Indicator on the Control Panel will become illuminated At this point place the hands inside the incubator using the glove ports see Working inside the cabinet with bare hands Page 1...

Page 14: ...nsation Plate and has several functions Firstly it is a safety device so that in the event of a gas Input Solenoid remaining open the gas has a way of getting out of the cabinet and not doing any dama...

Page 15: ...warmer gas is now forced down the channel at the back of the cabinet and passes under the bottom shelf of the incubator where it first meets the sachets of Conditioner and then the sachets of Catalyst...

Page 16: ...ove the hands from inside the cabinet simply collect the Bung and release it within the sleeve Remove the hand and let the Bung fall into the Cuff where it will seal Gravity will now hold the Bung in...

Page 17: ...y Description Recommended Replacement 2 Gauntlets 1 year 2 Cuffs 6 months 3 Fans 2 years 2 Heaters 3 years 1 Outer Door Seal 6 months 1 Inner Door Seal 6 months 1 Single Port Door Seal 6 months 2 Larg...

Page 18: ...et of catalyst housed in the roof of the lock This sachet can be both inserted and removed by bending it slightly and sliding it in or out of the holder CHANGING THE FLUORESCENT LIGHTING TUBE Switch o...

Page 19: ...E 30 PSI MIXED GAS FLOW 25 L MIN 33L LOCK FLUSH N2 GAS FLOW 25 L MIN 50L LOCK FLUSH MIXED GAS INPUT PIPE SIZE 6 mm DIA N2 GAS INPUT PIPE SIZE 6 mm DIA INCUBATOR POSITIVE PRESSURE SETTING 6 mm WG INCUB...

Page 20: ...44 45 Burnt Mill Elizabeth Way Harlow Essex CM20 2HU UK Description of Equipment Incubator for the environmentally controlled incubation of anaerobic bacteria I certify that the apparatus identified a...

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