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13 

THE REMOVAL OF CONDENSATION FROM INSIDE THE INCUBATOR 

 

 

Water vapour accumulates inside the incubator atmosphere from two sources, firstly from the evaporation 

of the media in the Petri dishes and secondly by the hydrogen and the traces of oxygen being linked 

together by the palladium catalysts. If this water vapour is not removed it will condense on the cooler 

surfaces of the incubator walls and form pools of water. The cabinet however is fitted with a large 

aluminium Condensation Plate which is kept a few degrees lower than the temperature inside the 

incubator by a fan which blows cool air from the room onto its outer surfaces. This reduction in 

temperature is sufficient to cause the excess water vapour inside the incubator to condense, run down its 

surface and collect at the bottom and finally fall via a drain tube into the collection bottle situated inside the 

cabinet. When this is full, remove it via the lock. 

 

The fan for the Condensation Plate can be switched "OFF" at the Control Panel if the Service Engineer is 

working on the unit. The humidity within the incubator is controlled by the Humidistat which is mounted just 

above the inner door. The Humidistat switches the fan "ON" and "OFF" automatically. 

 

 

 

THE GAS TRAP BUBBLE BOTTLE 

 

 

The Gas Trap Bubble Bottle is mounted on the incubator endplate just beneath the Condensation Plate 

and has several functions. Firstly, it is a safety device so that in the event of a gas Input Solenoid  

remaining open, the gas has a way of getting out of the cabinet and not doing any damage to the structure 

of the unit. Secondly, it allows the operator to be able to push their arms inside the cabinet without 

applying undue pressure. It must be remembered that all gas that gets out of the cabinet via the bubble 

bottle is wasted and therefore every effort must be made to minimise this when working inside the 

incubator. If one arm is withdrawn as the other arm is pushed into the unit, gas wastage can be reduced.  

 

 

 

THE HEATING AND GAS CIRCULATING SYSTEM 

 

 

The heating and gas circulating system consists of one tubular 400 Watt heaters fixed directly behind the 

fan units mounted on detachable plates in the top of the incubator. The fans run continuously whilst the 

heater is burst fired. 

 

 

 

THE TEMPERATURE CONTROLLER 

 

 

An adjustable electronic temperature controller fitted in the control panel of the cabinet regulates the 

temperature. The controller has a temperature sensor in the right hand top corner of the heating chamber 

and the temperature of the incubator can be adjusted between 3 degrees C above ambient to 40 degrees 

C. There is a green led incorporated in the temperature controller which indicates when the heater is 

operating and a temperature display to give an accurate reading of the internal temperature of the cabinet. 

A data sheet covering the temperature controller is included with this documentation. 

 

 

 

Summary of Contents for AW400TG TWO GAS

Page 1: ...ANAEROBIC WORKSTATION AW400TG TWO GAS VERSION MANUAL 44 45 Burnt Mill Elizabeth Way Harlow Essex CM20 2HU United Kingdom Telephone 44 0 20 8551 7000 Website www munroinstruments com...

Page 2: ...G THE GAS FLOW 11 13 TRANSFERRING ITEMS INTO THE INCUBATOR 12 14 TRANSFERRING ITEMS OUT OF THE INCUBATOR 12 15 SINGLE PLATE ENTRY 12 16 REMOVAL OF CONDENSATION FROM INSIDE THE INCUBATOR 13 17 THE GAS...

Page 3: ...lushing interlock 60 Petri dish capacity Condensation Plate Cooling air inlet Manually sealed interlock doors Control panel Anaerobic indicator bottle Single plate entry Omitted Clear acrylic window 5...

Page 4: ...Petri Dishes DOOR SEALING Manual GASES 1 Mixed Gas 10 H2 10 CO2 80 N2 2 Oxygen free Nitrogen N2 OXYGEN REMOVAL FROM AIRLOCK Positive Pressure flushing with catalyst FABRICATION MATERIAL Acrylic OVERA...

Page 5: ...re as follows MIXED GAS 25L min N2 25L min The diameter of the pipes connecting the cylinders to the cabinet should be selected according to the length of run to give a minimum pressure drop this will...

Page 6: ...rimary gauge showing pressure in bottle Regulator Gas isolation taps close to cabinet 6mm diameter flexible plastic tube 1m long Electrical supply 220 240V AC 50Hz Mount cabinet on strong bench at con...

Page 7: ...tor and fit collection pipe from cool plate Conditioner Catalyst Hang indicator pump here and plug into adjacent socket Clip anaerobic indicator bottle here Place conditioning sachet and palladium cat...

Page 8: ...to gauntlets Gauntlet holder Hang gauntlet on Gauntlet Hooks with Gauntlet Holders when not in use Fix gauntlet assembly to Arm Ports with O rings ensure there are no creases in the gauntlets undernea...

Page 9: ...d out of the cabinet without any fuss In fact the cabinet has been designed to be very user friendly SINGLE PLATE ENTRY LOCK This lock is used when only a small number of dishes need placing in the in...

Page 10: ...the bottle and place it in a bath of water and bring it to the boil The solution will change from being blue to colourless Tighten up the cap and remove the bottle from the water allow it to cool and...

Page 11: ...e cabinet Place the small Palladium Catalyst in the roof of the Lock See Sketch on Page 17 6 Now switch on the cabinet at the ELCB If either of the Low Gas Pressure neons is illuminated increase the a...

Page 12: ...Pressure Gauge 0 3 3 Bar Connect up the equipment as shown and start the Lock Flush Disconnect the gas supply to the cabinet at point X and insert a flow meter The meter should vent to the atmosphere...

Page 13: ...the Lock Anaerobic Indicator on the Control Panel will become illuminated At this point place the hands inside the incubator using the glove ports see Working inside the cabinet with bare hands Page 1...

Page 14: ...nsation Plate and has several functions Firstly it is a safety device so that in the event of a gas Input Solenoid remaining open the gas has a way of getting out of the cabinet and not doing any dama...

Page 15: ...warmer gas is now forced down the channel at the back of the cabinet and passes under the bottom shelf of the incubator where it first meets the sachets of Conditioner and then the sachets of Catalyst...

Page 16: ...ove the hands from inside the cabinet simply collect the Bung and release it within the sleeve Remove the hand and let the Bung fall into the Cuff where it will seal Gravity will now hold the Bung in...

Page 17: ...y Description Recommended Replacement 2 Gauntlets 1 year 2 Cuffs 6 months 3 Fans 2 years 2 Heaters 3 years 1 Outer Door Seal 6 months 1 Inner Door Seal 6 months 1 Single Port Door Seal 6 months 2 Larg...

Page 18: ...et of catalyst housed in the roof of the lock This sachet can be both inserted and removed by bending it slightly and sliding it in or out of the holder CHANGING THE FLUORESCENT LIGHTING TUBE Switch o...

Page 19: ...E 30 PSI MIXED GAS FLOW 25 L MIN 33L LOCK FLUSH N2 GAS FLOW 25 L MIN 50L LOCK FLUSH MIXED GAS INPUT PIPE SIZE 6 mm DIA N2 GAS INPUT PIPE SIZE 6 mm DIA INCUBATOR POSITIVE PRESSURE SETTING 6 mm WG INCUB...

Page 20: ...44 45 Burnt Mill Elizabeth Way Harlow Essex CM20 2HU UK Description of Equipment Incubator for the environmentally controlled incubation of anaerobic bacteria I certify that the apparatus identified a...

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