EDVOTEK® • 1.800.EDVOTEK (1.800.338.6835) • FAX: (301) 340.0582
www.edvotek.com • [email protected]
7
CREATE NEW PROGRAM, continued
5.
Enter the three PCR cycling steps.
6.
Press enter at the
Denaturation TEMP
and enter the desired temperature. Press enter at the
Denaturation TIME
and enter the desired time (i.e. for 30 seconds, enter the time as 0030).
7.
Press enter at the
Annealing TEMP
and enter the desired temperature. Press enter at the
An-
nealing TIME
and enter the desired time.
8.
Press enter at the
Extension TEMP
and enter the desired temperature. Press enter at the
Exten-
sion TIME
and enter the desired time.
9.
If a final extension is not desired, scroll down to the
Cycles
default (see below for entering the
number of cycles).
10. If a final extension is desired, press the enter button and enter the final ex-
tension temperature. Next, press the enter button again and enter the final
extension time.
Finally, press the enter button at the
Cycles
default.
Enter the number of cycles for the PCR cycling steps.
The number to the right is the total number of cycles and the number to the left
will display as the current cycle in progress.
DELETE SAVED PROGRAM
The EdvoCycler™ can hold 20 saved programs. From time to time it may be necessary to delete
old programs to make room for new programs. Remember, once a program is deleted, it cannot
be retrieved. You will need to re-enter the program (you may want to record the PCR cycling steps
for programs that are deleted).
1.
Scroll to
DELETE SAVED PROGRAM
and press enter.
2.
Scroll to the program you wish to delete and press enter. When prompted
“ARE YOU SURE?”
press enter
YES
to delete the program or scroll up or down to enter
NO
to keep the program.
TEMP HOLD PARAMS
From the Main Menu: TEMP HOLD PARAMS
Enter into that menu:
•
Temp Hold (scroll for on/off)
•
Set Hold Temp: 10
°
C or Higher (cannot go down to 4
°
C)
•
Return (enter here to go back to main menu)
NOTE: Running the thermal cycler on hold for long periods of time will shorten the life of the unit.
NOTE:
Time is entered
as seconds.
(For example:
30 sec. = 0030,
2 min. = 0120.)
