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Edvotek EdvoCycler, Quick Manual

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Edvotek EdvoCycler Quick Manual Download Page 5

EDVOTEK®  •  1.800.EDVOTEK (1.800.338.6835)  •  FAX:  (301) 340.0582

www.edvotek.com  •  [email protected]

5

MAIN MENU

> LOAD SAVED PROGRAM
   MODIFY SAVED PROGRAM
   CREATE NEW PROGRAM
   DELETE SAVED PROGRAM
   TEMP HOLD PARAMS

LOAD PROGRAM

> 

330 

336 341 669

   331 

337 

701 

000

   332 

952 

004 

000

   333 

961 

371 

111

   334 

962 

764 

000

PROGRAM 330 

TEMP    TIME

Init Denat
Denaturation  

94       0040

Annealing  

45       0045

Extension  

72       0045

Final Ext  

72       0300

Cycles 

 

  01       30

PROGRAM >000  TEMP    TIME
Init Denat  

00    0000

Denaturation  

00    0000

Annealing   

00    0000

Extension   

00    0000

Final Ext   

00    0000

Cycles 

 

     00    00  

TEMPERATURE HOLD MENU

   TEMP HOLD ON
> SET HOLD TEMP  

010

   RETURN

Quick Guide Screens

A

B

C

D

E

GETTING STARTED

Turn on the power switch (“|” mark is on, “O” mark is off).
Use the up or down button on the front panel to select the desired
function (the arrow to the left of the menu will allow you to choose
that selection).

The five options are as follows:

LOAD SAVED PROGRAM
MODIFY SAVED PROGRAM
CREATE NEW PROGRAM
DELETE SAVED PROGRAM
RUN PROGRAM

LOAD SAVED PROGRAM

Use this function if you are running
an EDVOTEK® kit.  The programs for
most EDVOTEK® PCR kits are pre-
loaded on the EdvoCycler™ and
ready-to-run.  Simply follow ED-
VOTEK instructions for preparing
DNA samples for PCR and place
tubes in the EdvoCycler™.  The
block holds only 0.2 ml PCR tubes -
if PCR sample is prepared in a
different tube, you must transfer
sample to a 0.2 ml thin-wall PCR
tube (supplied in all EDVOTEK®
PCR kits).

1.

Scroll to 

LOAD SAVED PROGRAM

 and press enter.

2.

Scroll up or down to the desired program and press enter. Check
with the kit instructions to make sure parameters match with
screen display.  If they do not, reference 

MODIFY SAVED PRO-

GRAM

  section and make the necessary changes to the existing

program.

continued

IMPORTANT

Always check the PCR cycling

parameters in the EDVOTEK®
manual that accompanies your

experiment.  Compare the

program to ensure that it

matches the pre-programmed

cycles.  If there has been a

change, it will be necessary to
modify the existing program to

match the new parameters.

Summary of Contents for EdvoCycler

Page 1: ...hase of the EdvoCycler Thermalcycler TABLE OF CONTENTS Introduction 2 Warnings 2 Operator Safety 2 Installation 2 After Use 2 User Maintenance 3 Guarantee 3 Contact Information 3 EdvoCycler Quick Guide 4 Getting Started 5 Load Saved Program 5 Modify Saved Program 6 Create New Program 6 Delete Saved Program 7 Temp Hold Params 7 Run Saved Program 8 Pause or Escape 8 ...

Page 2: ... procedures If the equipment is used in a manner not specified by EDVOTEK the protection provided by the equip ment to the user may be impaired All EDVOTEK units have been designed to conform to interna tional safety requirements and are fitted with an over temperature cutout The cutout is preset to protect the unit If a safety problem should be encountered switch off at the mains socket and remov...

Page 3: ... you may need to return the unit for repair The fuse is located in the back of the unit in the AC adapter Guarantee The unit is guaranteed against any defect in material or workmanship for two years Please fill out the warranty card as soon as possible to ensure proper warranty coverage The warranty period is from the date of purchase and within this period all defective parts will be replaced fre...

Page 4: ...gram and goes to hold WARNING Running the unit for long periods of time at high or low temperatures will shorten the lifespan of the unit Change values by pressing ENTER and keying in numerical values To modify the tempera ture time and number of cycles scroll to value to be changed press ENTER Using keys 0 9 punch in desired value NOTE need to keep same number of digits so if 45 sec enter 0045 On...

Page 5: ...DIFY SAVED PROGRAM CREATE NEW PROGRAM DELETE SAVED PROGRAM RUN PROGRAM LOAD SAVED PROGRAM Use this function if you are running an EDVOTEK kit The programs for most EDVOTEK PCR kits are pre loaded on the EdvoCycler and ready to run Simply follow ED VOTEK instructions for preparing DNA samples for PCR and place tubes in the EdvoCycler The block holds only 0 2 ml PCR tubes if PCR sample is prepared i...

Page 6: ...nge and press enter When the item disappears enter the new parameter 4 Scroll to another setting you wish to modify After you are satisfied with the changes to the program press the escape button and the changes will be saved Time is entered as seconds i e 30 seconds is displayed as 0030 2 minutes is displayed as 0120 5 To run the modified program scroll to LOAD SAVED PROGRAM and follow the direct...

Page 7: ... at the Cycles default Enter the number of cycles for the PCR cycling steps The number to the right is the total number of cycles and the number to the left will display as the current cycle in progress DELETE SAVED PROGRAM The EdvoCycler can hold 20 saved programs From time to time it may be necessary to delete old programs to make room for new programs Remember once a program is deleted it canno...

Page 8: ...temporarily hold a cycle at a certain point USE EXTREME CAUTION WHEN OPENING THE LID AND REMOVING SAMPLES FROM THE BLOCK THE HEATED LID AND BLOCK WILL BE VERY HOT 2 When ready to return to the program replace samples to the block close the lid and push the RUN button 3 To stop the program while it is running press the escape button Press the up or down button to YES and press enter to abort the pr...

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