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6
LOAD SAVED PROGRAM, continued
3.
Load tubes in block. If all spaces will be used, it may be necessary to “nest”
tubes (place tubes aligned with one another and facing the same direc-
tion). See Figure 1. Close lid.
4.
Press the RUN button. The lid starts to preheat or the cycling will begin im-
mediately.
5.
When the cycling steps begin, you can monitor the progress on the
screen display. The flashing arrow indicates the current status. The
bottom row (Cycles) shows current cycle to the left and the total # of
cycles to the right.
6.
To end the cycling at any time: Press the escape button and scroll
with the up or down button, enter
YES
to stop the experiment.
MODIFY SAVED PROGRAM
Use this function to modify or to make an adjustment to an existing pro-
gram.
1.
Scroll to
MODIFY SAVED PROGRAM
and press enter.
2.
Scroll up or down to the desired program and press enter.
3.
Scroll to the setting you wish to change and press enter. When the item disappears, enter the
new parameter.
4.
Scroll to another setting you wish to modify. After you are satisfied with the changes to the
program, press the escape button and the changes will be saved. Time is entered as seconds
(i.e. 30 seconds is displayed as 0030, 2 minutes is displayed as 0120).
5.
To run the modified program, scroll to
LOAD SAVED PROGRAM
and follow the directions running
a saved program.
CREATE NEW PROGRAM
Note - if an incorrect entry is made at any time during
CREATE NEW PROGRAM
, simply scroll up or
down to the wrong entry and press enter. When the item disappears, enter the correct parameter.
1.
Scroll to
CREATE NEW PROGRAM
and press enter.
2.
Press the enter button and then enter the new program number.
3.
If an initial denaturation is not desired, scroll down to
Denaturation
(see below for entering PCR
cycles).
4.
If an initial denaturation is desired, press the enter button and enter the
Initial Denaturation
temperature. Next, press the enter button again and enter the
Initial Denaturation
time (i.e. for
3 min., enter the time as 0180).
continued
Warning !
The heated lid
will get very hot –
use extreme
caution to avoid
getting burned
Figure 1
