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pump output. Put the inlet tubing and output capillary into same reservoir with solvent
isopropanol: water 1:1. Set flow rate to 50 ml/min, start pumping and set pressure at 10MPa using
reduction valve. Let the pumping for at least 30 min (optimal 2 h). Keep the flow rate at 50 ml/min
and set pressure at 20MPa. Let the pumping for at least 30 min (optimal 2h). Keep the flow rate at
50 ml/min and set pressure at 25 MPa. Let the pumping for at least 10 min (optimal 30 min). Keep
the flow rate at 10 ml/min and set pressure at 20 MPa. Let the pumping for at least 10 min (optimal
30 min). Seals are now set and run-in. Pump is ready for standard operation. Do not forget to wash
the isopropanol from the pump using your mobile phase. If the pressure decreases during
operation, it is necessary to run the pump in for a longer time (optimally). If you do not have a
reduction valve, you can use an old column, which meets the above stated parameters).
Caution:
Watch washing heads and all connections during run-in for any leakage.
Correct every uptightness!
In menu item
Menu/Service/Parts replacement/Seals
tick the seal that has been
replaced.
L PUMPING
– left working head seal
L WASHING
– left washing head seal
R PUMPING
– right auxiliary head seal
R WASHING
– right washing head seal
Continue by choosing
CONTINUE
. Press
ENTER
for confirming seal replacement. This way
operation counters zeroize in
Menu/Info/Parts/Seals
.
6.7.1.2. Check Valves
The basis of check valve is precise
ball and seat
placed
in a case made of PEEK with stainless steel reinforcement.
Liquid flow pushes the ball into the seat and this way it seals.
With regard to high pressures in the pump any
slight impurity
stuck on the ball or seat may cause
pressure fluctuation
or
the pump cannot start at all. If it is not possible to eliminate this
problem by repeated purge, it is necessary to exchange or
clean the check valves.
Flow direction through the valve is according to the arrows on
check valve side.
Disconnect the unit from power
source!!!
Disconnect inlet tubing.
To exchange inlet valve, loosen its
holder using side wrench 5/16"
(8 mm).
