Look through the
eyepiece
and SLOWLY turn the focusing knob until the image
of the specimen becomes clear. Be careful not to let the slide touch the lens.
Focusing Tips
When focusing your microscope turn the knob only until the stage
stops moving. DO NOT FORCE IT.
The
disc diaphragm
, located directly below the stage, has six different apertures
(openings), which allow various amounts of light to pass through. Rotating this
disc allows you to modify the amount of light that is transmitted. Try
experimenting with various settings to get the most effective view.
After focusing you can move the slide left or right on the stage to observe different
sections of the same specimen. As you adjust the slide or the aperture disc, be
careful not to put weight against the stage as this could push it out of focus. If this
occurs, simply readjust the focusing knobs slightly until you again have a clear
view.
6
After observing with the
4X objective
(which shows you the image magnified
40X), rotate the nosepiece to the
10X objective
lens (leave the slide in place on
the stage). SLOWLY turning the focusing knob will enable you to get a clear view
of your specimen at 100X magnification. Always use caution since the focusing
knob actually moves the stage closer to the objective lens.
Finally, you can turn the nosepiece to the
40X objective
, giving you a 400X
magnification of your slide. When increasing magnification, always remember that
the higher the magnification, the closer the objective must be to the specimen
being observed.
Focusing Tips
The 40X lens will appear to be almost touching the slide, this is
normal. Always move the focusing knobs very slowly to avoid breaking the slide
with the objective.
When changing slides, move the stage to its lowest position, lift the clips to
remove and replace slides and begin observing with the 4X objective again.
7
Nosepiece
disc diaphragm
