FEI Tecnai G
2
F20 Operating Procedures
(6/01/2017)
1. Startup
1.
Sign-up
in the microscope log-book
2.
Startup the software if not already running:
Start the software in this
sequence:
1.
Tecnai User Interface
2.
Digital Micrograph
3.
TIA
(TEM Imaging and Analysis)
3.
Check the vacuum
: Look at the
Vacuum
panel under the
Setup
menu. The
GUN, COLUMN and CAMERA values should look like the ones below and
highlighted in green. Gun will always be 1 Log.
4.
Fill the cold-trap dewar with LN
2
:
Cover the screen window first. Fill the dewar
until it is full. If you are the first user of the day, refill it again after 30 minutes.
Refill the LN
2
at least every 3 hrs to keep it full
5.
Inserting the specimen holder
Room temperature sample
There are two things to check prior to inserting the specimen holder:
(a) Under the
Search tab
, in the
Stage
2
menu, click on the flap-out menu and click
Control
then
Holder
. This will reset X, Y, Z, and
α
on the stage and avoid damaging the
