5
7) Filling the Micropipette
First, select your fill speed by pressing the [
SETUP
] icon and choosing
23
or
46 nL/sec
.
(NOTE: EMPLY SPEED IS PRESET TO 92 nL/SEC ONLY)
Return to Main Menu by pressing the [
EXIT
] icon.
Then enter manual mode by pressing the [
MANUAL
] icon.
Once the micropipette is secured to the collet, press the [
EMPTY
] icon. This will drive
the wire plunger out forcing oil to the tip of the pipette and any excess oil will be
expelled. Extend the plunger until an audible beep is heard. The plunger is now fully
extended (approx. 27 mm from the end of the collet).
Now place the tip of the pipette into your sample and press the [
FILL
] icon. The
plunger will retract drawing with it the sample. The micropipette when fully filled, will
contain approximately 4.2 µl of sample. At any time you can stop filling by pressing the
red [
STOP
] icon. Continued filling can be accomplished by pressing the [
FILL
] icon
again. The plunger is fully retracted to its preset “home” position when you hear an
audible beep. Viscous samples may require you to fill in small steps allowing the
sample to equilibrate in the tip before continued filling. Do not allow air bubbles to form
in the micropipette. These bubbles can cause inaccurate injection volumes.
NOTE: The plunger will continue to extend or retract until you press [
STOP
], or fully
extended or retracted position is reached.
8) Injecting Sample
To inject the sample, return to the Main Menu by pressing the [
EXIT
] icon. Press the
[
SETUP
] icon then choose your
Speed (nL/sec)
, either 23 or 46 nL/sec. Then press
the [
EXIT
] icon. Select inject mode by pressing the [
INJECT
] icon. Set the desired
injection “Volume (nL)” from the displayed volumes. Press the [
INJECT
] icon to inject
your sample.
NOTE: Multiple injections can be performed by simply pressing the [
INJECT
] icon
again and again.
Aluminum Barrel
Plunger
Sealing O-ring (small hole)
O-ring with large hole
Collet
Glass
Plastic sleeve (spacer) – Placed between
O-rings with indentation toward glass
Fig. 1
Summary of Contents for Nanoject II
Page 11: ...11 Notes...
