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1. The Principle:

The light source of the Epi-fluorescence device will eradiate light with specific wavelengths.

It will be transmitted on the sample and excite the sample to produce fluorescence. You may have a

high brightness and high contrast image to achieve fluorescence observation and analyze.

2. Function:

The range of the spectrum excited: 350nm

580nm

The range of the fluorescence spectrum: 420nm

650nm

3. Technical Parameters and Outfit:

Name

Specification

Quantity

Epi-fluorescence

device

B

blue

EX490

DM510

BA515

1Set

G

green

EX545

DM580

BA590

O

ordinary transmission light

Microscope body

Coaxial coarse and fine focusing mechanism; Double

layers mechanical stage; ABBE condenser; Built –in

halogen lamp with brightness adjustor. Koehler

illuminating system.

1Set

Viewing head

Articulated free binocular head; or Articulated free

trinocular head; or sliding binocular head; or sliding

trinocular head.

1Set

Eyepiece

Wide field plane-scope eyepiece :

WF10X/18

WF16X/13

1Pair/each

Objective

Achromatic objective for fluorescence device

4X

10X

40X(s)

100X(s, oil)

1Set

Power supply box

In: AC90-254V

Out: 100W DC

Fuse: 5A

1Set

Light source

Mercury lamp

HBO Ultra-high pressure mercury

spherical lamp: 100W

2Pcs

Halogen lamp

6V/20W Halogen lamp; Fuse: 1A

2Pcs

Power cord

1Pcs

Immersion oil

Special immersion oil for fluorescence device

1bottle

Ultraviolet bar

1Pcs

Service manual

1Copy

D) How to Use the Fluorescence Microscope:

1. Turn on the switch (17) of the power supply box (19). It will be in full working condition

after 10 minutes. The tension-guide line (16) will show the state of the mercury lamp.

2. Adjusting the center of the mercury lamp

Push the excitation type selector (5) to the position “o”, and push the flashboard B (6) in a

middle position. You will find a light shadow on the observation window (21). If the light shadow

is not in the middle of the window, rotate the screw (8) to move the shadow up/down, rotate the

screw (9) left/right.

3. Adjusting the condenser of the Epi-fluorescence device

P.4

Summary of Contents for DW-2002H

Page 1: ...EPI FLUORESCENT MICROSCOPE SERVICE MANUAL Read it carefully before operating and keep it with the microscope...

Page 2: ...oscope It is not only used for fluorescent microscopy observation but also used for general biological microscopy observation 2 No dismantle the equipment Unless you are a microscopic expert or there...

Page 3: ...the microscope otherwise it will damage the circuit and bulb even lead to insecurity 5 Protecting optical parts Never try to contact directly the optical surface of objectives eyepieces and other opt...

Page 4: ...Microscope P 2 C The Principle Function and Main Technical Parameters of the Fluorescence Microscope P 3 D How to Use the Fluorescence Microscope P 4 E Use Scope of the Fluorescent Filter P 5 F How t...

Page 5: ...let Bar 13 Fluorescent Objective 14 Microscope Body 15 Brightness Adjustor 16 Tension Guide Line 17 Switch 18 Accumulator 19 Power Supply Box 20 Ultraviolet Bar 21 Observation Window 22 Epi fluorescen...

Page 6: ...with soft cloth and gasoline promptly Remove the plastic cover away from the Epi fluorescence device 22 and install it on the microscope body 14 with the screw 11 Usually the lamp house in the Epi fl...

Page 7: ...the power supply box 19 and fix it 5 If the objectives are packed in plastic bottles please take them out and spin them in the nosepiece 6 Install the viewing head 3 on the Epi fluorescence device 22...

Page 8: ...lar head 1Set Eyepiece Wide field plane scope eyepiece WF10X 18 WF16X 13 1Pair each Objective Achromatic objective for fluorescence device 4X 10X 40X s 100X s oil 1Set Power supply box In AC90 254V Ou...

Page 9: ...lect the different spectrum range Pull the selector 5 out to the limiting position for G green light exciting state Push the selector 5 into the limiting position for B blue light exciting state Put t...

Page 10: ...nsequence Select appropriate lens Immersion oil produces the fluorescence Use special immersion oil The lamp in base is not turned off Turn off the lamp The lenses in collector reflect ambient light t...

Page 11: ...place Disconnect it with the power source and put the dust cover over it after using If it will be not used for a longer time it is the best way to screw the objectives out and place them into the le...

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