3
CONTENTS
SETTING UP THE SYSTEM
4
Software Installation .................................................................................................................. 4
Layout of the Hardware ............................................................................................................. 4
Recording the Positions of Motors ............................................................................................ 5
Unpacking ................................................................................................................................. 5
Electrical Connection ................................................................................................................ 5
Installing the Z and V Arms ....................................................................................................... 5
Rezeroing Motorized Syringe Drivers ....................................................................................... 6
Mounting 100
m
l Gas-Tight Syringes on Motorized Syringe Drivers, and
Filling them with Water .............................................................................................................. 6
Installing 2.5 ml syringe ............................................................................................................. 8
Testing the Performance of the Plateloader .............................................................................. 9
Realigning the Plateloader ........................................................................................................ 9
Rezeroing all Motors of the Plate Loader .................................................................................. 10
FRONT PANEL MENU ITEMS
11
USE AND MAINTENANCE OF THE HARDWARE
15
Installing Microtips ..................................................................................................................... 15
Use of Liquid Handling System Including Valve Positions ........................................................ 15
Refilling 100
m
l Gas-Tight Syringes with Water ....................................................................... 15
Debubbling ............................................................................................................................. 15
Flushing Tubing between Valves ............................................................................................ 16
Filling Microtip with Solution ................................................................................................... 16
Dispensing Experiments ......................................................................................................... 16
Attach the Evaporation shield for Vapor Diffusion Experiments ................................................ 17
Flushing Microtip after Use ....................................................................................................... 18
Always wear protective goggles when handling caustic materials ............................................ 18
Protein Coatings of Microtip ...................................................................................................... 18
Blockages.................................................................................................................................. 18
Chemical Inertness ................................................................................................................... 18
Tubing Connections .................................................................................................................. 18
Lubrication of Syringe Drivers and Plate Loader. ...................................................................... 20
Lubrication of Shafts ............................................................................................................... 20
Lead-screws ........................................................................................................................... 20
Spillages ................................................................................................................................. 20
XYZV Plate Loader ................................................................................................................... 20
Adjustment of XYZV Plate Loader .......................................................................................... 20
HARDWARE CONFIGURATION AND CONTROL
21
Using Different Syringes with the System ................................................................................. 21
Changing Plates ........................................................................................................................ 21
WASP and ASPRUN .............................................................................................................. 21
Pick And Mix ........................................................................................................................... 21
XSTEP – Changing Plates ..................................................................................................... 21
Adding Further Plates to PLATES.DAT .................................................................................... 21
Hardware Configuration Files .................................................................................................... 22
HARDWARE.CFG .................................................................................................................. 22
HARDWARE.FTH .................................................................................................................. 22
Specifications ............................................................................................................................ 24
INDEX OF MANUAL
25
