3
CONTENTS
Chapter 1 - Wire Myograph overview .................................................................................................................................................................................... 3
Chapter 2 - setting up the wire myograph - 620M ............................................................................................................................................................ 4
2.1 Changing and adjusting the mounting supports ...................................................................................................................................................... 4
2.1.1 Changing the mounting supports (figure 2.1) ......................................................................................................................................................... 4
2.1.2 Coarse adjusting the jaws for small vessels (figure 2.1) ........................................................................................................................................ 4
2.1.3 Fine adjusting the jaws for small vessels (figure 2.2 and figure 2.3) ................................................................................................................. 5
2.1.4 Fine adjusting the pins for larger vessels (figure 2.4 and figure 2.5): ............................................................................................................... 6
2.2 Calibration of the force transducer .................................................................................................................................................................................. 6
Chapter 3 - Experimental set-up ............................................................................................................................................................................................. 7
3.1 Mounting protocol for small arteries .............................................................................................................................................................................. 7
3.1.1 Mounting step one ............................................................................................................................................................................................................. 7
3.1.2 Mounting step two ............................................................................................................................................................................................................. 8
3.1.3 Mounting step three .......................................................................................................................................................................................................... 8
3.1.4 Mounting step four ............................................................................................................................................................................................................ 9
3.1.5 Mounting step five ............................................................................................................................................................................................................. 9
3.1.6 Mounting step six .............................................................................................................................................................................................................10
3.1.7 Mounting step seven .......................................................................................................................................................................................................10
3.2 Normalization ........................................................................................................................................................................................................................10
3.2.1 Principles of the normalization procedure ..............................................................................................................................................................11
3.3 Standard start ........................................................................................................................................................................................................................11
3.3.1 Principles of the standard start procedure ..............................................................................................................................................................11
3.4 Endothelium function ........................................................................................................................................................................................................12
3.4.1 Principles of checking endothelium function ........................................................................................................................................................12
3.5 In vitro experiment 1: Noradrenaline contractile response ..................................................................................................................................13
3.5.1 Background .........................................................................................................................................................................................................................13
3.5.2 Protocol ................................................................................................................................................................................................................................13
3.6 In vitro experiment 2: Acetylcholine relaxation curve ............................................................................................................................................14
3.6.1 Background .........................................................................................................................................................................................................................14
3.6.2 Protocol ................................................................................................................................................................................................................................14
Chapter 4 - Cleaning and maintenance ..............................................................................................................................................................................15
4.1 Cleaning the Wire Myograph ..........................................................................................................................................................................................15
4.2 Maintenance of the force transducer ............................................................................................................................................................................16
4.2.1 Checking the force transducer .....................................................................................................................................................................................16
4.2.2 Force Transducer Replacement ....................................................................................................................................................................................17
4.3 Maintenance of the linear slides .....................................................................................................................................................................................18
Appendix 1 - Buffer recipes .....................................................................................................................................................................................................19
Appendix 2 - Normalization theory ......................................................................................................................................................................................21
Appendix 3 - Reading a millimetre micrometer ...............................................................................................................................................................23
