DIATECH PHARMACOGENETICS EasyPGX qPCR instrument 96 User Manual Download Page 13

 
 

EasyPGX

® 

 qPCR instrument 96 

Cat.Nr: RT800-96 
 

13/21

 

 

                                                  

 2017/06 

 

 
 

HARDWARE FUNCTIONS 

LED INDICATOR 

On the front of the instrument (right upper corner) there is a LED status indicator.  The table below shows the color code 
for this indicator: 

LIGHT INDICATION 

MEANING 

Off 

The instrument is switched off 

Flashing green 

The instrument is running 

Fix green 

The instrument is paused 

Flashing red 

The instrument has detected an error. Check what is shown 
on the instrument display for more details 

 

LOADING THE HEATING BLOCK 

The instrument can be loaded with individual PCR tubes, strips or 96-well plates.  

  Open the instrument cover above the heating block using the handle to lift it. Open it completely. 

  Raise the heated lid by pulling the handle forward and then lifting it from the heat block. 

  Place the plate, strips or tubes on the block and check that they are correctly positioned. 

  Close the heated cover and push it back so that it locks properly. 

  Close the instrument cover and make sure it locks. 

  

 
 

SOFTWARE FUNCTIONS 

IMPORT OF TEMPLATES 

“EasyPGX

®

 qPCR instrument 96

 uses the dedicated 

Aria DX software

. The software is validated for use in 

combination with the "

EasyPGX

®

 ready

" kits manufactured and commercialized by Diatech Pharmacogenetics 

srl. It is recommended to use only pre-set templates available on request contacting Diatech Pharmacogenetics 
Technical Support (email

[email protected]

).  

 

 
Import of template should only be performed the first time you use the specific kit associated with that template. 
In subsequent runs it is sufficient to recall it (see paragraph "Execution of a run"). 
 

  Save the template coupled with the kit in use to a USB stick.  

  Switch  on  the  instrument  by  pressing  the  button  on  the  bottom  of  the  front  panel  and  wait  for  the  Home 

screen to appear. 

  Insert the USB stick on the door on the instrument panel. 

  On the Home screen, press "New Experiment". The "Experiment Types" screen opens. 

  Press "Open Template". The "Template" screen opens. 

  Press  "Import",  select  the  template  on  the  USB  stick  and  press  "OK".  The  template  is  then  automatically 

saved in the specific "Template" folder. 

  Remove the USB stick. 

 

EXECUTION OF A RUN 

  Switch  on  the  instrument  by  pressing  the  button  on  the  bottom  of  the  front  panel  and  wait  for  the  Home 

screen to appear. 

  On the Home screen, press "New Experiment". The "Experiment Types" screen opens. 

  Press "Open Template". The "Template" screen opens. 

  Press  on  the  template  file  associated  with  the  kit  you  are  using  to  select  it  and  press  "Open".  The  "Plate 

Setup" screen opens. 

  Press "Sync Plate" to synchronize the acquisition channels. 

  Open the "Thermal Profile" window. 

  Press "Run Experiment" to start the run. 

Summary of Contents for EasyPGX qPCR instrument 96

Page 1: ...tics srl requiring the use of EasyPGX qPCR instrument 96 in the respective user manual RT800 96 8057289090675 2017 06 Diatech Pharmacogenetics srl a Socio Unico Società soggetta all attività di direzione e coordinamento di Diatech srl con sede in Jesi Via Ignazio Silone 1 b 60035 Jesi AN Italy C F P Iva R Imprese di Ancona n 02483840423 Tel 39 0731 213243 Fax 39 0731 213239 info diatechpharmacogen...

Page 2: ...EasyPGX qPCR instrument 96 Cat Nr RT800 96 2 21 2017 06 This page was intentionally left blank ...

Page 3: ...ENVIRONMENT REQUIREMENTS 7 ELECTROSTATIC DISCHARGES 7 OTHER FEATURES 7 INSTALLATION 8 POSITION OF THE INSTRUMENT 8 UNPACKING OF THE INSTRUMENT 8 CLEANING THE HEATING BLOCK 8 CONNECT THE INSTRUMENT TO THE ELECTRIC POWER 8 CONNECT KEYBOARD AND MOUSE TO THE INSTRUMENT OPTIONAL 9 BACKGROUD CALIBRATION 9 DATE AND TIME SETTING 9 DIAGNOSTIC TEST 9 SOFTWARE INSTALLATION 10 ARIA DX SOFTWARE INSTALLATION 10...

Page 4: ...ION 15 TOUCH SCREEN CALIBRATION 15 INSTRUMENT MANTEINANCE 15 CLEANING THE INSTRUMENT 15 CLEANING THE OPTICAL MODULES 15 TECHNICAL SPECIFICATIONS 17 WARRANTY AND CLAUSE OF LIABILITY 18 WARRANTY 18 EXCLUSIONS 18 GUARANTEE LIMITATION AND REMEDIES 18 RESPONSIBILITY CLAUSE 18 APPENDIX A ELECTRIC AND ELECTRONIC WASTE DISPOSAL DIRECTIVE WEEE 19 OPERATOR NOTES 20 ...

Page 5: ...NOT PROVIDED USB pen drive USB 2 0 or higher if used also for execution of runs Personal computer Minimum requirements for running Aria Dx software Operating system Windows 7 Professional and Ultimate editions or Windows 10 Supported architectures o x86 32 bit supported on Windows 7 o x64 64 bit supported on Windows 7 and 10 Programs o Microsoft NET Framework 4 0 o Microsoft SQL Server 2012 only r...

Page 6: ...edical devices IVD only in countries that accept the user manual translated into the languages available on the web site www diatechpharmacogenetics com area riservata QUALITY ASSURANCE The EasyPGX centrifuge vortex 1 5 ml kit has been designed developed and validated in compliance with the Directive 98 79 EC on in vitro diagnostic IVD medical devices transposed in Italy in the D Lgs No 332 2000 a...

Page 7: ... to process inflammable explosive or reactive substances It is responsibility of the user to apply appropriate safety measures if potentially pathogenic material radioactive substances or other hazardous substances are used Do not immerse the instrument in any liquid DANGER OF BURNS Do not touch the thermoblock the inner part of the lid or the reaction tubes strips plates while the instrument is o...

Page 8: ...t stays supported on the box Lift the instrument and place it where it is expected The instrument weighs 23 kg To avoid damages to the instrument and people it is recommended to lift the instrument at least in two CLEANING THE HEATING BLOCK Whit the instrument cover opened clean the outer and inner surfaces of the heating block Lift the heat block door by pulling the handle forward and then upward...

Page 9: ... the Backgroud Calibration procedure fails contact Diatech Pharmacogenetics Technical Support email support diatechpharmacogenetics com phone 39 0731 213243 DATE AND TIME SETTING In the lower right corner of the display press the displayed date and time A menu window shows up press Change Date and Time In the window that appears set the correct date and time Click on the Help icon to see detailed ...

Page 10: ... diatechpharmacogenetics com to check for compatibility Otherwise simply install the Aria DX software on the computer that will be used for the analysis of the results To check the minimum requirements of the PC refer to the section Material Required but Not Provided The standard Windows Installer setup msi is available on request from Diatech Pharmacogenetics Technical Support email support diate...

Page 11: ...yPGX qPCR instrument 96 Cat Nr RT800 96 11 21 2017 06 The Application Mode window opens select AriaDX and press Next The Setup Type window opens Select Standard and press Next Do not change this selection ...

Page 12: ...17 06 The Destination Folder window opens Press Next to continue Press Install The wizard installs the AriaDX software in the folder shown When the installation is complete the InstallShield Wizard Completed window opens Click Finish to close the wizard ...

Page 13: ...armacogenetics srl It is recommended to use only pre set templates available on request contacting Diatech Pharmacogenetics Technical Support email support diatechpharmacogenetics com Import of template should only be performed the first time you use the specific kit associated with that template In subsequent runs it is sufficient to recall it see paragraph Execution of a run Save the template co...

Page 14: ...aved Experiment Select the last run file from the list Press Copy Select USB Disk on the left Press Paste Return to the home screen by pressing the Home button at the bottom left of the display and disconnect the USB stick ANALYSIS OF THE RESULTS For the analysis of the results obtained with the kit of the EasyPGX ready line refer to the user manuals that can be downloaded from the private area of...

Page 15: ...to optimize sensitivity On the Home screen press Settings In the Settings screen press Calibrate Touch The next screen shows how to use a marker Follow the instructions on the screen to complete the procedure INSTRUMENT MANTEINANCE CLEANING THE INSTRUMENT To clean EasyPGX qPCR instrument 96 follow the steps below Use a cloth dampened with water or isopropyl alcohol to clean the outside of the inst...

Page 16: ...yPGX qPCR instrument 96 detects an error it displays a special icon at the bottom of the touch display Press this icon In the popup menu that opens press Count X where X matches the number of error messages to read A table opens with the following information o Type message type error or warning Warning messages are used for errors that do not prevent the execution of experiments o ID Error Identi...

Page 17: ...Relative humidity 20 80 without condensation Maximum altitude 2000 m Excitation source 8 LEDs for each optical module Detection 8 photodiodes for each optical module Channels FAM HEX Power supply 100 240 VAC 50 60 Hz 1100 VA Thermal system Peltier block Positions 96 Temperature range of the thermal system 25 0 99 9 C Uniformity 0 2 5 C above 60 C 0 4 C 40 59 C Maximum heating speed 6 C s Maximum c...

Page 18: ...ND REMEDIES The warranty previously described is the unique and exclusive guarantee of Diatech Pharmacogenetics srl and the repair or replacement of defective parts is the sole and exclusive right of remedy No other warranties no express or implied warranties are provided The implied warranties of merchantability and eligibility for particular purposes are explicitly excluded extended to all categ...

Page 19: ...or environment The end user of the products must certify this with a specific Declaration of Decontamination which must be handed over to the carrier The End User is responsible for the deletion and or removal of any sensitive data contained in the product being returned Shipping costs will be charged to the Customer unless otherwise agreed with Diatech Pharmacogenetics Packaging conditions All pr...

Page 20: ...EasyPGX qPCR instrument 96 Cat Nr RT800 96 20 21 2017 06 OPERATOR NOTES ...

Page 21: ...Diatech Pharmacogenetics srl a Socio Unico via Ignazio Silone 1 b 60035 Jesi AN Italy Tel 39 0731 213243 Fax 39 0731 213239 info diatechpharmacogenetics com www diatechpharmacogenetics com ...

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