34
Release:
09/2008
CyBi
®
-Well
Initial
Start-Up
&
Routine
Start-Up
Procedure
Inspect
liquid
level
of
all
pipetting
tips
after
two
hours.
Document
all
tips
with
a
varying
liquid
level.
In
the
case
of
a
CyBi
®
-Well
384/25
μ
L,
visual
testing
is
difficult
to
perform.
Follow
a
procedure
similar
to
that
of
→
“Precision Test”
in
this
case:
Dispense
the
nomi-
nal
volume
completely
into
a
384-well
transparent
flat-bottom
microplate
with
prefilled
buffer
solution
on
expiry
of
the
test
time
described
above
and
measure
extinction
in
a
vertical
photometer.
The
volume
which
is
dispensed
per
pipetting
tip
should
not
deviate
from
the
mean
value
by
more
than
20%.
Result of leak testing
CyBi
®
-Well 96/250
μ
L and CyBi
®
-Well 96/25
μ
L:
There
must
be
no
perceivable
difference
in
the
filling
height
of
the
various
pipetting
tips
on
completion
of
testing.
CyBi
®
-Well 384/25
μ
L:
There
must
be
no
individual
variances
>-20%
on
completion
of
testing.
A
drop
in
the
fill
level
of
a
tip
suggests
a
potential
leak
in
this
tip.
Repeat
this
test
with
new
pipetting
tips.
If
the
leak
is
found
to
occur
in
the
same
place,
you
should
contact
the
manufacturer
or
your
service
partner.
NOTE
Keep
the
pipetting
tips
submerged
during
leak
testing,
in
order
to
prevent
formation
of
droplets
at
their
ends,
because
these
may
falsify
the
result
of
testing.
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