Covaris E220 evolution User Manual Download Page 1

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E220

evolution

User Manual

Page 1

For research use only

E220

evolution

USER MANUAL

Instrument and software developed for
high performance sample preparation
using adaptive focused acoustic energy

Product Name:

E/LE220-Series

Model Numbers:

E220

evolution

Software: SonoLab 7

Summary of Contents for E220 evolution

Page 1: ...r research use only E220 evolution USER MANUAL Instrument and software developed for high performance sample preparation using adaptive focused acoustic energy Product Name E LE220 Series Model Number...

Page 2: ...1721 USA Printed in U S A UNIVERSAL PRECAUTIONS Universal Precautions should be followed on all specimen samples regardless of whether a sample is known to contain an infectious agent Laboratories han...

Page 3: ...ent the system is designed to operate with a water bath and re circulating heater chiller apparatus to control sample temperature NEVER run a method without a water bath this could damage the transduc...

Page 4: ...s and the medium on which the software is delivered is free of defects in materials under normal use and service The warranty is void if damage has resulted from third party software not intended for...

Page 5: ...Edit Methods 20 4 2 Temperature Tab 25 4 3 Saving Methods in Folders 26 4 4 History Tab 27 4 5 Setup Screen 27 4 6 Maintenance Screen 32 4 7 About Tab 33 4 8 Motion Tab 33 5 0 SYSTEM SPECIFICATIONS 35...

Page 6: ...ocused ultrasonicators are based on Covaris Adaptive Focused Acoustics AFA technology This instrument is designed to provide researchers an integrated stand alone tool to be used in a laboratory envir...

Page 7: ...ontaining the instrument Main Unit Quick Start User Guide User Manual Notebook Computer with AC Adapter Cables o USB cable o Instrument power cord o Computer AC Adaptor power cord Accessories o Chille...

Page 8: ...trument and the laptop The recirculating chiller may be placed on the floor to save bench space 2 2 2 Please refer to the rear of the instrument s right side panel and identify the following features...

Page 9: ...essories to the computer 2 2 7 Connect the two hoses provided for coolant flow from the chiller The connections are bi directional There is no defined IN or OUT 2 2 8 Connect the hoses to the chiller...

Page 10: ...er tank and remove it from its plastic shipping bag Remove and discard the desiccant pack Safety Enclosure and Water Tank 2 2 12 If a Water Conditioning System WCS will be employed with the instrument...

Page 11: ...external chiller programming its set point to a few degrees below the desired instrument bath temperature Be sure to employ a 20 glycol solution inside the chiller to prevent freezing Do not use undil...

Page 12: ...An animated splash screen will appear Following the splash screen the SonoLab Run Screen will appear with the motion system homing dialog box in front of it Click Yes The transducer and sample holder...

Page 13: ...ce of the instrument model In this case please refer to Section 7 0 for diagnostic instructions 3 3 The SonoLab Run Screen Running a Method 3 3 1 Instrument Status Basic Controls System attributes are...

Page 14: ...the instrument Water levels near or below 0 on the WATER LEVEL label will cause this indicator to turn red Door Open A green check indicates that the door is properly closed Motors Enabled indicates...

Page 15: ...here must be an acoustic method selected in the Method box The Degas pump must be running The Degas pump timer must see the pump running for 60 minutes or override Water Temperature must be within acc...

Page 16: ...umerically and graphically at the lower right corner of the Run Screen along with a progress bar for the method being run Note that the average Power values will approximate Peak Power x Duty Factor T...

Page 17: ...en please refer to the next section on System Shutdown 3 4 System Shutdown If the instrument will be idle for an extended period of time e g overnight or a weekend shut down the instrument using the f...

Page 18: ...mple temperature Each protocol published by Covaris specifies a desired bath temperature The instrument does not control its own water temperature This must be controlled by adjusting the set point of...

Page 19: ...h 3 6 Degas System The instrument is equipped with a pump that draws bath water through a degassing process Properly degassed water is important for efficient transfer of acoustic energy from the inst...

Page 20: ...nfiguration information The Run tab is the principal user screen for the system See details presented earlier in this section on operating the instrument through the Run Screen The Run Screen The Run...

Page 21: ...appropriate plate depends on the tubes being used in your application Create Method Edit Method Screen 4 1 3 Click the Add Step button The Add Method Step window will appear The Step Types available...

Page 22: ...0 Watts Duty Factor During treatments acoustic power is burst or turned on and off according to Duty Factor and Cycles per Burst The duty factor is the percentage of time that the ultrasound signal is...

Page 23: ...atment Choosing a Delay step introduces a fixed time delay into a method The user can set the sample to settle between two different treatment steps The delay is specified in seconds Add a Delay Step...

Page 24: ...ate Allows the user to duplicate a selected step Group Steps Allows the user to create a group with one or more steps A group may be executed once or repeated multiple times A Create Step Group window...

Page 25: ...Enabling User Authentication for details 4 2 Temperature Tab From the Edit Existing or Create New Method screen the Temperature tab may be selected for more advanced temperature settings The default t...

Page 26: ...ll close the Create or Edit screen 4 3 4 Locked If User Authentication is enabled a Specialist or Administrator level user may lock a method against editing by another user by clicking the Locked icon...

Page 27: ...disable user authentication and configure user accounts Specify folders used to save methods history files and log files Schedule the degas pump auto start stop time 4 5 1 Communications SonoLab will...

Page 28: ...is enabled by an administrator users log in under their User Name and Password when working with the system Users not logging in will have the lowest user rights of an anonymous Operator To enable Use...

Page 29: ...can additionally create edit or remove user accounts To create a new user simply click Add New User and type the new User Name Password and User Role Operator Specialist Administrator into the New Us...

Page 30: ...ings For this change to take effect you must exit and restart SonoLab To enable or change the automatic Deletion of Log Files Enable User Authentication and log in as an Administrator Navigate to Setu...

Page 31: ...he Windows Task Scheduler Login window appears Log in under your Windows user name Computers provided by Covaris have a single Windows user Covaris with no password Click OK on Successfully Save the S...

Page 32: ...ansducer Parameters are typically used only by Covaris service personnel and are read only screens for SonoLab users 4 6 1 Firmware Update On occasion it is necessary for Covaris to make changes to th...

Page 33: ...ons but not the factory definitions delivered with the instrument Users should contact Covaris personnel before creating new well plate definitions Plate Editor Screen 4 7 About Tab The About Tab prov...

Page 34: ...he sample tray to predefined positions The Stop button halts movement of the motion system The Test Limit Switches button is a troubleshooting tool As the name implies it tests the motion system limit...

Page 35: ...0 1 2004 and CAN CSA C22 2 No 61010 1 2004 2nd Edition Safety Requirements for Electrical Equipment for Measurement Control and Laboratory Use Part 1 General Requirements EMC Complies with Class A Ind...

Page 36: ...to the instrument and operate the degas pump for a few seconds to purge the bleach solution from the pump lines and housing 6 Empty the tank rinse and fill with fresh DI water 7 Place the water tank b...

Page 37: ...r below about 15 degrees C does not degas as efficiently Typically the operator should begin degassing approximately 30 minutes prior to powering on the chiller If it is necessary to have the instrume...

Page 38: ...ing items before contacting Service o Water Level Verify that the water level in the tank is at or above the 0 mark on the WATER LEVEL label Verify that the Acoustic Assembly is fully lowered into the...

Page 39: ...e of the tank The inlet holes very small drilled holes in the side of the tube should be free of obstruction o If the degas pump makes a very loud noise when operating it may need to be replaced Parti...

Page 40: ...rigorously tested at the Manufacturing Facility prior to shipment This is done to ensure that your Covaris device is of the highest quality and meets your needs for consistent efficient and reliable S...

Page 41: ..._____________________________ Corrective Action ______________________________________ YES NO Three 3 appropriate power outlets are available for the device laptop and accessory Chiller if necessary Y...

Page 42: ...SonoLab and is approximately the same as the ambient temperature since tank is empty Bath Temp Reading ______ C YES NO With transducer in position in empty tank verify the presence of a red next to W...

Page 43: ...Treatment Time 30 Seconds Duty Factor 5 0 Peak Incident Power 100 W Cycles Burst 50 Click SAVE AS and enter Method Test YES NO Click Run on the SonoLab Screen to start the recently created Method Ver...

Page 44: ...cted Power value by the following Expected Power Peak Incident Power X Duty Factor 100 Expected Power _________________ Watts Per standard practice allow bath water to Degas at least 60 minutes and co...

Page 45: ...ssolved Oxygen Meter i e HACH HQ Series to measure the baseline oxygen level Baseline Dissolved Oxygen Level ________ PPM mg L Turn Chiller OFF or disconnect from instrument Install water bath in devi...

Page 46: ...s the correct voltage rating for the installation WARNING To prevent the possibility of electrical shock always plug the system into a grounded power source Water Requirements The instrument s water b...

Page 47: ...E220 evolution User Manual Page 47...

Page 48: ...double click on the Sonolab 7 shortcut SonoLab will open its splash screen as described in Section 3 of this manual Installing Well Plates 210050 1 To install the standard set of plate definitions ope...

Page 49: ...ndows will automatically install the drivers and pop up the following message onto its desktop Drivers for several devices within the instrument will be installed sequentially This may take several mi...

Page 50: ...Manual Page 50 Under Other devices right click first device RF Controller Board and select Update Driver Software When the driver software update dialog opens select the option to Browse my computer...

Page 51: ...ed Click next You may be notified that Windows can t verify the publisher of this driver software Click on Install the driver software anyway Windows will install the driver and post the following dia...

Page 52: ...ftware as shown in the image below Repeat the installation steps shown above Return to Other devices and repeat this process in order top to bottom for the 3 remaining entries until all Covaris device...

Page 53: ...rnatively users may purchase a chiller separately It is recommended that the chiller have a capacity of at least 250 Watts at a set point of 5 C and able to deliver a minimum pump pressure of at least...

Page 54: ...Covaris CH 04 Chiller PolyScience AD 07 has a reservoir capacity of 7 liters Add about 1 5 liters of glycol to the empty reservoir then fill to within an inch of the top for approximately a 20 mix Ple...

Page 55: ...inner wall of the tank Employing the optional WCS with an E or LE System precludes the need for a daily change of bath water It is however important that water which evaporates each day be made up wi...

Page 56: ...E220 evolution User Manual Page 56 7 Turn the WCS off and disconnect hoses Raise instrument to service position 8 Empty the water tank and dry 9 Gently dry transducer and surfaces of instrument...

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