20
1ml Distilled Water
TEMED ready solution
Stock 4 X Sample Buffer
4ml glycerol
2ml 2-mercaptoethanol
1.2 g SDS
5ml 4 X Stacking Tris
0.03 g Bromophenol blue
Aliquot into 1.5ml microcentrifuge tubes. Store at -20°C.
Towbin Buffer
25mM Tris,
192mM glycine,
20% methanol pH8.3,
Towbin Buffer SDS
25mM Tris
192mM glycine
20% methanol pH8.3
0.05-0.1% (w/v) SDS
Bjerrum and Schafer-Nielsen Buffer
48mM Tris
39mM glycine
20% methanol pH9.2
Dunn Buffer
10mM NaHCO3
3mM NaCO3
20% methanol pH9.9
