15
40-90mA
Three gels
90-225V
60-135mA
Four gels
90-225V
80-180mA
Gel Removal
1.
Turn the power supply off when the loading dye reaches the bottom
of the gel, sooner if your proteins are below 4 kDa in size.
2.
Remove the gel running module, first emptying the inner buffer into the
main tank. Buffer can be re-used but this may affect run quality if
continued.
3.
Unscrew the glass plates with the Screw version. To open the sliding
door version, insert the CSLKEY into the recess arch of the clamping
door. Twist key applying pressure to both the clamping door and the
CVS10D side cheek. The door will now click open. Repeat this process
until you have opened both the doors.
4.
Remove the glass plates. Then using CSLKEY separate notched and
the plain glass plates. Place the wedged end of the key between the
two plates and gently twist until the plates pull apart. The gel will
usually stick to one of the plates and can be removed by first soaking
in buffer and then gently lifting with a spatula.
5.
The gel is now ready to be stained with Coomassie or silver stain or the
proteins in the gel can be transferred to a membrane by
electroblotting for specific band identification and further analysis.
