Cleaver Scientific CVS10CBS, Instruction Manual

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10
Gel Volumes for the omniPAGE Mini Unit are shown below:
Number of gels Gel Thickness (mm) Volume (ml)
Single – one gel, one
dummy plate
0.5
1.0
1.5
2.0
3.8
7.5
11.3
15.0
Double – two gels 0.5
1.0
1.5
2.0
7.5
15.0
22.5
30.0
Using a Triple Plate
sandwich
– four gels
0.5
1.0
1.5
2.0
10.0
30.0
45.0
60.0
Prepare gel solutions as per tables below. These give the volumes of solutions
from the standard stock solutions. These should be gently mixed avoiding
generation of bubbles which will inhibit polymerization by removing free
radicals.
Resolving Gel, for 2 x 1mm thick gels:
Solution 5 % 7.5% 10 % 12% 15 % 17.5%
Distilled Water 8.7ml 7.5ml 6.3ml 5.25ml 3.75ml 2.5ml
30 % Stock Acrylamide
Solution 2.5ml 3.75ml 5ml 6ml 7.5ml 8.75ml
4 X Resolving Tris Solution 3.75ml 3.75ml 3.75ml 3.75ml 3.75ml 3.75ml
Add 15 µl of TEMED and 150µl 10 % Ammonium Persulphate to the resolving
gel solution before pouring to initiate polymerisation