Notes
iCell Cardiomyocytes User’s Guide
5
Chapter 2.
Handling and Storage
Handling iCell Cardiomyocytes
iCell Cardiomyocytes are provided as cryopreserved single-cell suspensions in
1.5 ml cryovials. Upon receipt, directly transfer the cryobox containing iCell
Cardiomyocytes to the vapor phase of a liquid nitrogen storage dewar. CDI strongly
recommends transferring the entire cryobox into the storage rack to avoid
transferring individual vials.
Handling iCell Cardiomyocytes Media
iCell Cardiomyocytes Plating Medium and iCell Cardiomyocytes Maintenance
Medium are shipped frozen on dry ice. Upon receipt, store iCell Cardiomyocytes
media at -20°C until ready for use and at 4°C for up to 2 weeks after thaw. If media
will be used for longer than 2 weeks, aliquot and freeze again after the initial thaw.
Do not subject media to more than a single refreeze and thaw cycle.
It is critical to maintain cryopreserved iCell Cardiomyocytes at a stable
temperature. Minimize exposure of cryopreserved iCell Cardiomyocytes to
ambient temperature when transferring vials to liquid nitrogen storage.
