Cellular iCell User Manual Download Page 19

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iCell Cardiomyocytes User’s Guide

 15 

 

however such failure is caused; or (iii) if Customer fails to comply with any of the provisions 
of Appendix A in this User’s Guide; or (iv) if there is any abuse, other misuse or neglect of 
the Products by Customer or to the extent of any damage or loss of the Products by events 
or occurrences beyond a person’s (e.g., Cellular Dynamics’) control including without 
limitation, accident, fire, vandalism and natural disasters (acts of God). This warranty applies 
only to Customer and not to third parties. This warranty is not assignable.  

C. TO THE FULLEST EXTENT PERMITTED BY APPLICABLE LAW, CELLULAR 
DYNAMICS DISCLAIMS ALL REPRESENTATIONS, AND WARRANTIES, EXPRESS OR 
IMPLIED, WITH RESPECT TO THE PRODUCTS, INCLUDING BUT NOT LIMITED TO, 
ANY IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR 
PURPOSE OR NON-INFRINGEMENT, AND CUSTOMER WAIVES ALL RIGHTS AND 
REMEDIES, WITH RESPECT TO ANY DEFECTIVE PRODUCTS OTHER THAN THE 
EXPRESS WARRANTY AND REMEDY THEREFOR STATED ABOVE IN THIS 
APPENDIX B.  

D. Within five (5) business days of thawing the Product but prior to the expiration date of the 
Product as listed on the Certificate of Analysis and/or Product’s label (the “Warranty 
Period”), Customer must notify Cellular Dynamics in writing of any nonconformity of the 
Products, describing the nonconformity in detail. Customer’s failure to properly notify Cellular 
Dynamics in the Warranty Period voids the limited warranty set forth above in this 
Appendix B.  

E. Customers who believe they have a warranty claim should call Cellular Dynamics’ 
Technical Support line at (608) 310-5100 ext. 5 or email at [email protected] 
to request a replacement Product based on a breach of the limited warranty set forth above 
in this Appendix B. Any action by Customer for Cellular Dynamics’ breach of this limited 
warranty, for which Customer has given timely and proper notice of such breach during the 
Warranty Period and otherwise in accordance with this Appendix B, must be commenced by 
Customer within 18 months following the date of such breach.  

F. Cellular Dynamics makes no warranty of any kind or nature, neither express nor implied, 
for any product sold together with, or as a part of, the Products (e.g., an accessory 
accompanying a Product or a discrete component part of a Product that is a kit) that is not 
manufactured by Cellular Dynamics. Any such accessory to or part of the Products shall 
have the warranty, if any, that is offered and granted (and, for clarity, extended by its terms 
to Customer) by the manufacturer of such other accessory or component product 
accessories. 

G. Customer acknowledges and agrees that Cellular Dynamics may fill Customer’s order 
with any number of units of Products. Such units may be more units than Customer ordered. 
Customer will not be charged extra for any adjustments made by Cellular Dynamics. The 
number of cells in a unit is determined by the Product’s Certificate of Analysis. The number 
of cells that are contained in a unit accounts for both viability and plating efficiency 
percentages. Because this may vary from lot to lot, Cellular Dynamics reserves the right to 
fill the order with that number of units which is sufficient to fill Customer’s order and such 
adjustments shall not constitute a breach of the limited warranty set forth herein. 

Summary of Contents for iCell

Page 1: ...iCell Cardiomyocytes User s Guide...

Page 2: ...s not control and is not responsible for any Third Party Information including without limitation the content accuracy copyright compliance compatibility performance trustworthiness legality decency l...

Page 3: ...ring Cell Culture Surfaces 6 Preparing 0 1 w v Gelatin Solution 6 Preparing the Gelatin Cell Culture Vessel 6 Chapter 4 Thawing Medium and iCell Cardiomyocytes 7 Thawing iCell Cardiomyocytes Plating M...

Page 4: ...s are for life science research use only See Appendix A for more information and other restrictions A Safety Data Sheet SDS for dimethyl sulfoxide DMSO in which iCell Cardiomyocytes are frozen is avai...

Page 5: ...s instructed in this User s Guide iCell Cardiomyocytes will begin to beat spontaneously within 24 48 hours When plated at appropriate densities iCell Cardiomyocytes also will form electrically connect...

Page 6: ...romoter No RFP expression 1 Safety Data Sheets and User s Guide available online at www cellulardynamics com lit 2 Available online at www cellulardynamics com coa Required Equipment and Consumables I...

Page 7: ...ur inquiries In addition hands on training is available www cellulardynamics com training or watch our Handling iCell Cardiomyocytes Training Video www cellulardynamics com cm_handling In lab training...

Page 8: ...ely transfer to LN2 storage Culture Preparation Culture Maintenance Handling and Storage p 5 Thawing p 7 Plating p 9 Replacing the Medium p 13 Experimental Utilization native biology drug discovery di...

Page 9: ...als Handling iCell Cardiomyocytes Media iCell Cardiomyocytes Plating Medium and iCell Cardiomyocytes Maintenance Medium are shipped frozen on dry ice Upon receipt store iCell Cardiomyocytes media at 2...

Page 10: ...are 0 1 gelatin solution by dissolving 500 mg of gelatin Type A powder cell culture tested in 500 ml of sterile water in each bottle 3 Sterilize the gelatin solution by autoclaving using liquid cycle...

Page 11: ...fore thawing to ensure maximal performance of cells Complete the following steps of the thawing procedure in a time efficient manner to facilitate optimal iCell Cardiomyocytes viability and performanc...

Page 12: ...x the contents of the 50 ml centrifuge tube by inverting 2 3 times Gentle mixing is critical to ensure maximum viability Avoid vigorous shaking or vortexing of the cell suspension Note Thaw up to 3 vi...

Page 13: ...essel Plated Cell Count The number of viable cells that have firmly attached to the cell culture vessel at 48 hours post plating Plating Efficiency 100 Example If 600 000 cells were seeded and 350 000...

Page 14: ...d must be determined empirically based on the intended use However a density of 63 000 cardiomyocytes cm2 provides a beating syncytium and is the recommended starting density for most cell based assay...

Page 15: ...l formats by substituting the appropriate recommended cell numbers and seeding volumes 1 Prepare the thawed cell suspension for plating into a 96 well cell culture plate a Obtain the Plating Efficienc...

Page 16: ...e added to a 96 well cell culture plate at the indicated seeding densities to obtain the desired plating densities The cardiomyocytes were allowed to recover for 48 hours and then labeled with calcein...

Page 17: ...es while gently washing the surface of the plate Note Alternatively aspirate the Plating Medium to remove the non adherent cells and perform 2 washes with the appropriate volume of Maintenance Medium...

Page 18: ...or any cells derived developed or expanded therefrom in humans in clinical trials for diagnostic purposes involving human subjects or for any investigational or other therapeutic use Customer shall no...

Page 19: ...Dynamics Technical Support line at 608 310 5100 ext 5 or email at support cellulardynamics com to request a replacement Product based on a breach of the limited warranty set forth above in this Append...

Page 20: ...VER CAUSED AND REGARDLESS OF FORM OF ACTION WHETHER IN CONTRACT TORT INCLUDING NEGLIGENCE STRICT PRODUCT LIABILITY OR OTHERWISE EVEN IF CELLULAR DYNAMICS HAS BEEN ADVISED OF THE POSSIBILITY OF SUCH DA...

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