Notes
iCell Cardiomyocytes User’s Guide
13
Chapter 6. Maintaining iCell Cardiomyocytes
iCell Cardiomyocytes are shipped cryopreserved, at high purity. The
cardiomyocytes preserve a high purity for at least 2 weeks after thawing if plated in
iCell Cardiomyocytes Plating Medium (Plating Medium) and maintained in iCell
Cardiomyocytes Maintenance Medium (Maintenance Medium) as recommended.
1.
24 hours before use, thaw the Maintenance Medium overnight at 4°C.
2.
Immediately before use, equilibrate the Maintenance Medium in a 37°C water
bath.
3.
48 hours post-plating iCell Cardiomyocytes, gently wash off the non-adherent
cells and debris by pipetting the Plating Medium up and down approximately
5 times while gently washing the surface of the plate.
Note:
Alternatively, aspirate the Plating Medium to remove the non-adherent
cells and perform 2 washes with the appropriate volume of Maintenance
Medium, gently washing the surface of the plate with each medium change.
4.
Aspirate the Plating Medium and replace with the appropriate volume of
Maintenance Medium. Be careful not to touch or disrupt the adhered
cardiomyocytes. See Table 2 on page 10 for recommended seeding volumes
for several common cell culture vessels.
5.
Replace the Maintenance Medium every other day.
6.
Culture iCell Cardiomyocytes in a cell culture incubator at 37°C, 7% CO
2
.
Thaw the Maintenance Medium at 4°C overnight before its use. This medium
is stable for 2 weeks when stored at 4°C. Aliquoting the medium into single
use volumes post-thaw and subsequent storage at 4°C in tightly sealed
containers will minimize pH changes.
