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7. Slicing with HeartWare

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Summary of Contents for INKREDIBLE

Page 1: ...1 INKREdIBLE User manual CELLINK AB Arvid Wallgrens Backe 20 Gothenburg SE 41346 SWEDEN...

Page 2: ...man tissues and it is onlyyour imagination that limits what you can do This manual will serve as your guide for setting up your INKREDIBLE 3D Bioprinter maintaining it and also notifying you about saf...

Page 3: ...g your INKREDIBLE 3D Bioprinter 20 5 1 Setting up for first time use 22 5 2 Starting a print 23 5 3 Advanced features 26 6 How to install and setup HeartWare 28 6 1 Installing Heartware PC 30 6 2 Inst...

Page 4: ...4 1 Safety information...

Page 5: ...5...

Page 6: ...d through appropriate training Use the instrument only for its intended purpose as described in the documentation Do not modify the instrument sub components or accessories 1 1 General safety informat...

Page 7: ...teristics of the material and its compatibility with the dispensing unit The information can be found in the material safety data sheets packaging labels manufacturer or dealer catalogues Always use a...

Page 8: ...8 2 Introduction to your INKREDIBLE 3D Bioprinter...

Page 9: ...9...

Page 10: ...construct is built Once the construct has been bioprinted it is crosslinked using the UV LED curing system or ionic solutions depending on your bioink s crosslinking requirements 2 1 Specifications 2...

Page 11: ...mm 19 1 x 16 5 x 14 7 in Shipping Box 59 x 55 x 43 cm 23 x 21 5 x 17 in Shipping Weight 20 kg 44 5 lbs 2 1 4 Electrial Power Supply Adapter Input 100 240VAC 50 60Hz 2 0A Output 24VDC 6 67A 160W Max Po...

Page 12: ...12 3 Unpacking your INKREDIBLE 3D Bioprinter...

Page 13: ...13...

Page 14: ...nting the axes to move during transportation can be removed Once all protective materials have been removed the INKREDIBLE can be placed at its intended place of operation for example inside a biologi...

Page 15: ...nical nozzles GA 25 2x White conical nozzles GA 27 1x Straight stainless steel nozzle GA 22 1x 24 well plate 1x Glass petri dish 1x UV protective goggles 1x Tube with adapter to air compressor 1x Air...

Page 16: ...16 4 Product map...

Page 17: ...17...

Page 18: ...18 INKREdibLE 2a 2b 5a 5b 6 9 8 1 7a 7c 7b 3 4 10a 10b 13 14 12 1 1a 7c 7b 1 1b...

Page 19: ...d reader 7a Printbed adjustment butterfly screw 7b Printbed adjustment butterfly screw 7c Printbed adjustment butterfly screw 8 Control knob 9 Main LCD display 10a Cartridge air connector for printhea...

Page 20: ...20 5 Operating your INKREDIBLE 3D Bioprinter...

Page 21: ...21...

Page 22: ...e surface of the printbed right in front of the adjustment screw to the back left 7a 4 Leaving the printbed where it is move the printheads manually so that nozzle is right in front of the adjustment...

Page 23: ...NKREDIBLE on with the power button 1 2 Use the following sequense to home the INKREDIBLE 3D Bioprinter 3 Remove all objects from the printbed 4 such as well plates petri dishes etc 4 Lower the printbe...

Page 24: ...knobs straight out from the side of the INKREDIBLE 3D bioprinter 2 Turn down the pressure in both printheads to 0 by turning the pressure regulators 11a and 11b counter clockwise until they reach a co...

Page 25: ...der to have continuous flow simply turn the pressure regulators 11a and 11b Once the bioprinting process is completed the printbed will lower WARNING The INKREDIBLE 3D Bioprinter includes moving parts...

Page 26: ...luding UV protective safety goggles must be used with the UV curing system 5 3 2 Bioprinting using dual printheads To bioprint using dual printheads first control the following 1 When converting your...

Page 27: ...27...

Page 28: ...28 6 How to install and setup HeartWare...

Page 29: ...29...

Page 30: ...of finding the HeartWare install file Insert the SD card that came with your machine and copy the file setupHeartWare_2_4_1 zip to your PC Refer to the electronic version of this manual click the foll...

Page 31: ...he Python package you want installed However leaving the default options is strongly recommended 6 Click Finish to continue the installation of HeartWare 7 You may select Next past the next several pr...

Page 32: ...nality of HeartWare can be resolved by completely uninstalling and reinstalling HeartWare In addition to update HeartWare the old version must be completely uninstalled 1 Click the Start icon on the b...

Page 33: ...ll through the list till you find Cellink HeartWare version 2 4 1 5 Press Yes on the following three prompts and HeartWare will be uninstalled This will not uninstall Python 2 6 or remove your Slic3r...

Page 34: ...ng the print from the Repetier Host for Mac software 1 Navigate to the Repetier Host page by either Go to the CELLINK Support page at https cellink com support click Downloads and select Download next...

Page 35: ...e it into the Applications folder in Finder 4 Open the Repetier Host Mac file to start the install process A security message may appear because the Repetier Host Application is from an unrecognized d...

Page 36: ...36 5 Open the System Preferences 6 Go to Security Privacy...

Page 37: ...37 7 Click Open Anyway 8 Click Open...

Page 38: ...pen Repetier and read through the First Steps to familiarize yourself with the app Then follow the setup instructions in 6 3 Setting up HeartWare Repetier Host to configure your software to your INKRE...

Page 39: ...rtWare starts for the first time select Skip this Version 2 Start by clicking on Printer Settings 3 Copy the settings in the images below for any INKREDIBLE series bioprinter 6 3 Setting up HeartWare...

Page 40: ...40...

Page 41: ...41 4 Once you are done press Apply then click OK...

Page 42: ...42 7 Slicing with HeartWare...

Page 43: ...43...

Page 44: ...rouped in threes to represent triangles Edge to edge contact of these triangles forms a mesh that defines the exterior of an object 7 1 2 Slices The INKREDIBLE is a pneumaticallydriven bioprinterthat...

Page 45: ...models and exit HeartWare View Snap camera to specific orientations with respect to the print area and toggle appearance of visualizations within the View Window 3 Config Language printer and HeartWar...

Page 46: ...ea Move Object Sets the left click drag behavior of the mouse to move selected models across the printable area in the XY plane only Zoom Sets the left click drag behavior of the mouse to move the cam...

Page 47: ...nter point of the current view as the axis of rotation When Move Object from the Left Tool Bar is active Left MB Drag a selected object in the XY plane You cannot select objects by clicking the Left M...

Page 48: ...Print Preview When selected the visualization in the View Window will change to the most recently generated or loaded G Code for the current HeartWare session Save or run loaded G Code and view estim...

Page 49: ...Load icon In the Right Control Window click Add Object Your View Window will look like the image below with the cylinder replaced with the model of your choice 2 Now you can perform transformations on...

Page 50: ...d Click Reset to return the model to its original size Rotate Object Rotate an object around the X Y or Z axes using degrees This can also be used to level the object with respect to what HeartWare de...

Page 51: ...lds blood vessels tubules or skin patches These provide a basis from which settings can be fine tuned to better suit the materials mechanical properties porosity or other characteristics you may need...

Page 52: ...ftware will automatically switch to the Print Preview tab and the View window will look like the image below The blue lines depict the path that the printhead will follow to extrude print your part Yo...

Page 53: ...ance navigating this section Save to File This function store will store the G Code file in a local directory on the PC This is useful for reusing the G Code or modifying it using an external software...

Page 54: ...54 8 Maintenance and troubleshooting...

Page 55: ...55...

Page 56: ...he lead screw and liquid IP lubrication should be used to lubricate the carriage rods To lubricate the INKREDIBLE 3D bioprinter follow these steps 1 Turn off the printer and disconnect it from the pow...

Page 57: ...of the printer 9 Gently pull the X carriage all the way to the front 10 Gently push the printhead carriage all the way to the right 11 Lubricate the exposed segments of the carriage rods 12 Gently pu...

Page 58: ...the control knob and selsct Resume bioprint 8 2 2 The Bioprint option is not showing up in the menu The issue is most likely that the SD card was or is not properly inserted To fix this do the followi...

Page 59: ...ead 1 not turning of during non printing movements above 8 2 7 Dual head bioprinting Printhead 1 and printhead 2 are not level after the Raise printheads option has been selected The issue is most lik...

Page 60: ...rect this pull the knob gently towards you 8 2 10 Printhead 1 is missing the probing sensor at the end of the Home Axes sequence At the end of the Home Axes sequene the nozzle in PH1 touches the probi...

Page 61: ...ove Axis and enter by pressing the control knob 8 Select Move 0 1mm and enter by pressing the control knob 8 Select Move Y and enter by pressing the control knob 8 Jog the Y axis by turning the contro...

Page 62: ...62 9 Warranty and guarantee...

Page 63: ...63...

Page 64: ...at our own expense Proper packaging is required in the rare case of a returned product or part and the product itself must arrive back in the condition in which it was originally received CELLINK is...

Page 65: ...lf will be subjected to unavoidable scratching and wear upon use If you wish to order replacement parts they are available in our online store or by contacting us personally by telephone or email Ther...

Page 66: ...ined in calculation with the FedEx tracking information Limitations This guarantee and limited warranty are sole and final as they stand in reference to INKREDIBLE CELLINK and it s products where perm...

Page 67: ...67...

Page 68: ...68 You cannot create new science unless you realize where the old science leaves off and new science begins and science fiction forces us to confront this Michio Kaku...

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