Cellink INKREDIBLE, User Manual

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5.3 Advanced features
5.3.1 Crosslinking using the UV LED
If you wish you can crosslink bioprints using the built-in UV LED crosslinking system of
the INKREDIBLE 3D Bioprinter. To do so follow these steps:
1. Manually place the UV LED [3] as close as possible to the top of the bioprinted
construct.
2. Select the “Utilities Menu” and press the control knob to enter [8].
3. Select “Turn ON LED” and press the control knob to turn on the UV LED. To turn off
the UV LED curing, simply select “Turn OFF LED” and press the control knob [8].
The crosslinking time will vary depending on the bioink used and the thickness of
layers/construct.
WARNING! Always wear UV protective goggles and gloves, and secure
the environment of the instrument to other personnel while operating the
INKREDIBLE. All safety equipment, including UV protective safety goggles,
must be used with the UV curing system.
5.3.2 Bioprinting using dual printheads
To bioprint using dual printheads, first control the following:
1. When converting your 3D model to G-code, verify that you are using a profile
that supports using dual printerheads.
2. Verify that both cartridges have the same length nozzle attached.
CAUTION! The INKREDIBLE 3D Bioprinter CAN NOT bioprint using
nozzles with different lenghts! Doing this can damage your INKREDIBLE
3D Bioprinter!
3. Verify that printhead 1 and printhead 2 are completely level with each other:
-Select the “Utilities Menu” in the menu and enter by pressing the
control knob [8].
-Select “Home printheads” by pressing the control knob [8].
-Select “Raise printheads” by pressing the control knob [8].
-If the printheads are not completely leveled, follow the instructions
in chapter 8.2.7 “Dual head bioprinting: Printhead 1 and printhead 2 are
not leveled with each other after the “Raise printheads” option has been
selected”.