Cellink 000000020566, User Manual, Page: 48 / 60

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06 Frequently asked questions
• What is the mechanism that enables bioprinting with this printhead?
This printhead compresses the bioink within a cartridge. The cartridge is connected to an electromagnetic valve that can open and close for a
set length of time. Droplets are expelled from the printhead at the open and close rates set by the user.
• What do the LED colors indicate?
The printhead LED will change depending on its status. When the printhead is first put on the printhead mount, the light will turn white to
indicate that it is initializing. Once recognized, the LED will turn blue. The blue light also indicates a temperature below 35 degrees Celsius.
When the temperature of the printhead is between 35 and 50 degrees, the light turns yellow to indicate that the printhead is warm. The light
turns red at temperatures above 50 degrees to indicate that the printhead is hot.
• Can I use various printhead and printhead types simultaneously?
You can use multiple printheads in the same printing protocol to generate multimaterial constructs. However, you cannot print simultaneously
as pressure can only be provided to one printhead at a time.
• My bioink is not extruding.
The needle/nozzle may be clogged due to the bioink being used or a pause in its use. Replace the needle/nozzle. If your bioink contains
aggregates, ensure that they are of a sufficient size to pass through the needle/nozzle without clumping and blocking it. If using a
thermosensitive bioink it may have gelled in the tip. Consider using the Temperature-controlled Printhead to print thermosensitive bioinks.
• The droplets are not landing where they are supposed to on the surface.
If the droplets are deflecting away or not getting dispensed at all, you might not be using enough pressure. Try increasing the pressure or using a
contact nozzle with a needle.