Cellink 000000020566 User Manual Download Page 31 | Manualshive

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Cellink 000000020566 User Manual Download Page 31

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04 Getting started

3. The pressure can be adjusted within the cartridge to control the expulsion rate of the droplet.

• Certain bioinks require a minimum pressure to be expelled.
• A pressure that is too high can cause an expulsion rate that is too high, resulting in splattering of the droplet on the surface.
• A pressure that is too low might cause material to accumulate at the valve tip, or deflected droplets.

4. Use the Dispense button to test droplet expulsion and optimize your printing parameters.

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Summary of Contents for 000000020566

Page 1: ...BIO X EMD Printhead User Manual ...

Page 2: ......

Page 3: ...nsertion of microvalve and nozzle 16 4 4 Reassembling the printhead 19 4 5 Loading a cartridge 20 4 6 Testing Extrusion 25 4 7 Your first bioprint 28 4 8 Removing printhead 35 4 9 Printing optimization 37 5 Relevant G code commands 39 6 Frequently asked questions 43 7 Maintenance 45 7 1 Cleaning 46 7 2 Long term maintenance 49 Appendix A Consumables needles and nozzles 51 Available nozzles and par...

Page 4: ......

Page 5: ...1 Package contents 01 ...

Page 6: ...6110020558 1 Cartridge airline adapter with transparent tube 3cc 12 cm 000000010040 1 EMD microvalve 000000020551 1 Threaded nozzle needleless D16110020555 1 Threaded nozzle with needle 000000020560 1 Needle cap 000000020556 1 Flushing tube with Luer adaptor 20 cm D16110020912 000000010110 1 3cc cartridge inside EMD CSC010300102 1 ...

Page 7: ...3 Technical specifications 02 ...

Page 8: ...Recommended operating temperature 20 23 degrees Celsius NOTE We recommend operating BIO X in temperatures between 20 and 23 degrees In normal operating conditions the chamber temperature of the BIO X will heat up 2 3 degrees above room temperature Running temperature in recommended conditions 26 32 degrees Heating capacity and rate Total heating range 30 60 degrees Heating rate Room temperature 40...

Page 9: ...ssuming a nozzle length of 0 mm Exact build volume depends on the cartridge s position in the printhead and the nozzle needle used Build volume may be lower when combining the EMD Printhead with a printhead that restricts printbox move ment Please consult manuals for the specific printheads used to determine potential restrictions ...

Page 10: ... 4 mm 4 mm 12 8 cm 8 5 cm 6 5 cm 702 2 cm3 Table 1 Theoretical build volume based on nozzle length Actual volume may vary based on build plate s thickness size shape and level as well as on cartridge position and tightness of the nozzle X Y and Z are measured from the front left corner of the printbed ...

Page 11: ...7 Safety information 03 ...

Page 12: ...inthead mount manually will damage the motor If the mount needs to be moved place the printhead in the loading position described in the Getting Started section Use the proper method to load and unload the EMD Printhead onto the BIO X printhead mounts Instructions are outlined in the Getting Started section While loaded on the BIO X do not pull or push the printhead with excessive force especially...

Page 13: ...9 03 Safety information Figure 2 Do not touch the outlined area of the EMD microvalve ...

Page 14: ... the printhead mount The cartridge will be pressurized upon attachment and untwisting these components will result in bioink leakage Particles or pigment contained in the bioinks can limit the lifetime of the microvalve Contaminants or dirt particles can clog the microvalve and other components of the EMD Printhead ...

Page 15: ...11 Getting started 04 ...

Page 16: ...ters please consult the documentation for the bioink being used 4 1 Unpacking 1 Open the package Remove the pre assembled EMD Printhead and other components Figure 3 2 The pre assembled EMD printhead contains components A B C D and F Component E is provided in a separate protective container and component G is a tool for the removal of component F ...

Page 17: ...tting started Figure 3 Package contents include A EMD Printhead body B Microvalve C Nozzle cover for storage D Needleless threaded nozzle E Threaded nozzle F Luer lock adapter G EMD accessory tool A B C D E F G ...

Page 18: ...lize the bottom of the printhead mount with one hand to avoid straining the motor Align the printhead above the desired printhead mount and push downward using your opposite hand Figure 4 Note for more information on how to assemble the printhead see Section 4 3 Figure 4 Inserting the EMD Pneumatic Printhead into the BIO X system ...

Page 19: ...interface navigate to the Utilities menu and then go to the Tools submenu 3 Lower the desired printhead mount into the active or loading position by pressing the down arrow Figure 5 Figure 5 Lowering the printhead into the active position ...

Page 20: ...sertion of microvalve and nozzle 1 Ensure the printhead has been removed from the BIO X Section 4 2 2 Remove the nozzle cover from the EMD Printhead to reveal the nozzle Figure 6A 3 Unscrew and remove the threaded nozzle Figure 6B D E 4 Grip the edge of the microvalve Figure 6F being careful not to contact the glass tip Figure 2 Pull straight down to remove the microvalve from the EMD Printhead Fi...

Page 21: ...17 04 Getting started Figure 6 A Removing the nozzle cover from the EMD Printhead B D E Removing the nozzle from the EMD Printhead C F G Removing the microv alve from the EMD Printhead A B C D E F G ...

Page 22: ...e securing the printhead with your other hand Figure 7 B E Pull the tool out with the Luer lock attached Figure 7 C F Figure 7 Removing the Luer lock adapter A D Insert the removal tool into the EMD printhead B E Fit the Luer lock and twist counterclock wise until loose C F Pull out and remove the adapter A B C D E F ...

Page 23: ...wise until you feel resistance NOTE It s normal for a few threads to be visible Do not use excessive force or tools but ensure the nozzle is tight as a loose nozzle can unscrew during printing Figure 8 A B E F Figure 8 Assembling the EMD Print head A Inserting the microvalve into the EMD Printhead B Gently push until resistance is felt C E Align and tighten the nozzle to the printhead D F Tighten ...

Page 24: ...not overtighten or the threads on the adapter may be stripped and leakage could occur 4 5 Loading a cartridge 1 Obtain a prefilled bioink cartridge or fill a 3 mL cartridge with a low viscosity bioink Figure 9A 2 Remove the end top cap Figure 9B Figure 9 Preparing the cartridge for insertion into the EMD Printhead A B ...

Page 25: ...21 04 Getting started 3 Insert the cartridge into the EMD Printhead without connecting the printhead to the BIO X Figure 10 Figure 10 Inserting and tightening the cartridge into the EMD Printhead ...

Page 26: ...22 04 Getting started 4 Connect the cartridge to the pneumatic adapter by twisting the adapter on the end of the cartridge Figure 11 Figure 11 Attaching the 3 mL pneumatic adapter to the cartridge A B ...

Page 27: ...23 04 Getting started 5 Connect the air tubing to the respective air inlet on the printbox above the respective printhead Figure 12 Figure 12 Attaching the EMD Printhead to the BIO X printhead mount ...

Page 28: ...24 04 Getting started 6 Connect the printhead to the BIO X while stabilizing the mount Follow the instructions in Section 4 2 Figure 13 Figure 13 Connect the printhead to the BIO X A B ...

Page 29: ...25 04 Getting started 4 6 Testing Extrusion 1 Navigate to the Utilities menu and select the Tools submenu Figure 14 Adjusting the EMD Printhead parameters in the Utilities menu ...

Page 30: ...s button to adjust the open and cycle time Figure 15 Open time The length of time the valve is open during one cycle Cycle time The time between each opening that determines the droplet deposition frequency Figure 15 Adjustable parameters for the EMD Printhead ...

Page 31: ...nimum pressure to be expelled A pressure that is too high can cause an expulsion rate that is too high resulting in splattering of the droplet on the surface A pressure that is too low might cause material to accumulate at the valve tip or deflected droplets 4 Use the Dispense button to test droplet expulsion and optimize your printing parameters ...

Page 32: ...28 04 Getting started 4 7 Your first bioprint 1 Prepare the cartridges Section 4 5 2 Select Bioprint from the Start menu Figure 16 Figure 16 Start menu Select Bioprint to proceed print to setup ...

Page 33: ...29 04 Getting started 3 Select an STL file extension stl from the Model menu and proceed to the next menu Figure 17 Figure 17 Selecting STL file from the Model tab ...

Page 34: ...30 04 Getting started 4 Select a surface to print on Proceed to the next menu Figure 18 Figure 18 Select a print surface from the Surface menu ...

Page 35: ...thead is selected under the tool type Figure 19 6 Enter the desired printing parameters for the printhead Figure 19 and proceed to the Layers menu Parameters necessary for the EMD Printhead include Pressure Print speed Open time Cycle time Figure 19 Printing parameters to set on the Printer menu ...

Page 36: ... to the respective layer characteristics and proceed to the next menu Figure 20 Figure 20 Layer menu You can assign toolheads to respective print areas like perimeter infill and support You can select the infill pattern and density and preview the layers ...

Page 37: ...e pressure setting Figure 21 Press Print to proceed to the calibration page Figure 21 Print menu You can preview your printing parameters and test the pressure You can also adjust parameters on this menu Press Print to proceed to the calibration page and press Save to save the configura tion as a protocol ...

Page 38: ...o the desired printing height Figure 22 A calibration height of 1 3 cm is recommended for the needleless threaded nozzle and 0 mm for the needle threaded nozzle 10 Start the bioprinting process Figure 22 Figure 22 Bioprint menu You can calibrate the system and begin the bioprinting process ...

Page 39: ...dge by detaching the airline from the printer Figure 23A 4 Remove the printhead from the BIO X Use one hand to secure the printhead mount and the other hand to remove the printhead 5 Disconnect the tubing by twisting the cartridge adapter and lifting it off the cartridge Figure 23B 6 Twist and remove the cartridge The Luer lock adapter will be removed in one piece with the cartridge ...

Page 40: ...36 04 Getting started Figure 23 Depressurizing and removing the EMD Printhead A B ...

Page 41: ...he user to enter the open time and the cycle time Due to the technique used to form the droplets this printhead might be incompatible with highly viscous materials However adding heat to thermosensitive materials can lower the viscosity enough to make them compatible Larger nozzle diameters and valve travel are recommended when using larger flow volumes and high viscosity fluids while smaller diam...

Page 42: ...ult in a larger distance between droplets The EMD Printhead includes a noncontact nozzle and a contact nozzle The contact nozzle must be calibrated to the height of the surface while the noncontact nozzle must be calibrated between one and three centimeters away from the surface The contact nozzle has a needle which can extrude droplets or filaments depending on the parameters set In addition EMD ...

Page 43: ...39 Relevant G code commands 05 ...

Page 44: ... the printhead in mm min G4 Snnn Pnnn The G4 command instructs the system to dwell The S command is the wait time in seconds and P is the wait time in milliseconds G7 Xnnn Ynnn Ennn Fnnn When used in combination with the G90 command which defines absolute coordinates G7 is the relative move command The values for the X and Y parameters are the coordinates in mm directing where to move relative to ...

Page 45: ...M771 Tx Pyy M771 turns on the heater in printhead Tx at temperature Pyy where yy is a temperature between 30 and 65 degrees Celsius M750 Tx Py Dz Extrude from printhead x at pressure y for z milliseconds M751 Tx Stop extrusion from printhead x M2065 Tx Sy Set valve open time of printhead x to y microseconds M2067 Tx Sy Set valve cycle time of printhead x to y microseconds M400 M400 command causes ...

Page 46: ...42 ...

Page 47: ...43 Frequently asked questions 06 ...

Page 48: ...t temperatures above 50 degrees to indicate that the printhead is hot Can I use various printhead and printhead types simultaneously You can use multiple printheads in the same printing protocol to generate multimaterial constructs However you cannot print simultaneously as pressure can only be provided to one printhead at a time My bioink is not extruding The needle nozzle may be clogged due to t...

Page 49: ...45 Maintenance 07 ...

Page 50: ...dapter to remove residue Let it air dry 5 Connect a clean syringe to the provided pneumatic tubing 6 Pour deionized water or ethanol into a plastic cup If using water use a mild temperature 7 Retract the water ethanol into the syringe Figure 25A 8 Connect the end of the tubing to the microvalve Figure 25B 9 Pressurize the syringe by pushing on the plunger and inserting the microvalve into the adap...

Page 51: ...47 07 Maintenance Figure 25 Preparing the microvalve for cleaning Figure 26 Flushing the microvalve A B C D ...

Page 52: ...et it air dry 12 Connect the nozzle to the provided tube Use the syringe to flush with water or ethanol then water Hold the nozzle tightly when flushing to prevent it from disconnecting from the tube Figure 27 13 When the parts have dried reassemble the printhead for storage Section 4 2 Figure 27 Connecting the nozzle for cleaning ...

Page 53: ...eaded nozzles and Luer lock adapter can be autoclaved and cleaned with ethanol They have sufficient chemical resistance to ethanol and are temperature resistant for autoclaving Regularly clean the printhead with a damp cloth to remove any dust or debris ...

Page 54: ...50 ...

Page 55: ...51 Appendix A Consumables needles and nozzles ...

Page 56: ...m 0 15 mm 0 30 mm standard Art no D16110020555 Available microvalves for contact and noncontact jetting Threaded sleeve used for noncontact jetting where fluid is shot directly from microvalve end Available microvalve diameters 0 10 mm 0 15 mm 0 20 mm 0 30 mm standard 0 45 mm 0 60 mm Valve travel stroke 0 03 mm 0 06 mm 0 10 mm standard 0 15 mm ...

Page 57: ...eedles and nozzles Common and proven valve diameter travel combinations 0 1 0 06 travel 0 1 0 03 travel 0 15 0 03 travel 0 15 0 06 travel 0 2 0 06 travel 0 2 0 1 travel 0 3 0 1 travel standard Art no 000000020551 0 6 0 15 travel ...

Page 58: ...010311502 Empty cartridges 3cc with end and tip caps 50 pcs CSO010311102 UV shielding cartridges 3cc with end and tip caps 10 pcs CSO010311502 UV shielding cartridges 3cc with end and tip caps 50 pcs OH000000010 Female female Luer lock adapter 10 pcs OH000000050 Female female Luer lock adapter 50 pcs PSB030000502 Beige pistons 3cc 50 pcs PSO030000502 Orange pistons 3cc 50 pcs ...

Page 59: ...55 Support information Official site Sales Store Support Official site www cellink com Contact support cellink com Contact sales cellink com Web store www cellink com store ...

Page 60: ...www cellink com ...

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