Cell biolabs ViraBind Series Product Manual Download Page 8

 

to infect 293 cells, GFP positive cells were scored by counting after three days.  A: AAV Supernatant; 
B: Flow through; C: 1

st

 wash; D: Elution.

   

 

 

Figure 2

Electrophoretic Profile of Purified AAV2-GFP.

   

 

1.

 

Rabinowitz, J, and Samulski, R. J. (1998) 

Curr. Opin. Biotechnol

., 

9

, 470-475. 

References 

2.

 

Summer ford, C., and Samulski, R. J. (1999) 

Nat. Med., 5, 587-588.

 

3.

 

Clark, K., Liu, X., McGrath, J., and Johnson, P. (1999) 

Hum. Gene Ther

., 

10

, 1031-1039. 

 

1.

 

Tsuneoka, Y. et al. (2015). Distinct preoptic-BST nuclei dissociate paternal and infanticidal 
behavior in mice.

 

 EMBO J.

 doi:10.15252/embj.201591942. 

Recent Product Citations 

2.

 

Nishikawa, T. et al. (2015). Resetting the transcription factor network reverses terminal chronic 
hepatic failure.

 

 J Clin Invest.

 

 doi: 10.1172/JCI73137. 

3.

 

Stankowska, D. L. et al. (2015). Neuroprotective effects of transcription factor Brn3b in an ocular 
hypertension rat model of glaucoma.

 

Invest Ophthalmol Vis Sci.

 

 

56

:893-907. 

4.

 

Inaba, Y. et al. (2014). Gadd34 regulates liver regeneration in hepatic steatosis. 

Hepatology.

 

6

:1343-1356. 

5.

 

Moshiri, F. et al. (2014). Inhibiting the oncogenic mir-221 by microRNA sponge: toward microrna-
based therapeutics for hepatocellular carcinoma. 

Gastroenterol Hepatol Bed Bench

7

:43-54. 

6.

 

Inaba, Y. et al. (2014). Gadd34 regulates liver regeneration in hepatic steatosis. 

Hepatology

.  doi: 

10.1002/hep.27619. 

7.

 

Rodriguez, J.P. et al. (2014). 

Abrogation of β

-Catenin signaling in oligodendrocyte precursor

 

cells 

reduces glial scarring and promotes axon regeneration after CNS injury. 

J Neurosci

34

:10285-

10297. 

Summary of Contents for ViraBind Series

Page 1: ...Product Manual ViraBind AAV Purification Kit Catalog Number VPK 140 10 preps FOR RESEARCH USE ONLY Not for use in diagnostic procedures...

Page 2: ...ntly higher in vitro infection rates across a variety of cells and tissues Recombinant AAV 2 and AAV DJ vectors can be purified by CsCl gradient ultracentrifugation iodixanol discontinuous gradient ul...

Page 3: ...3 Assay Principle...

Page 4: ...L sterile tubes Purification Buffer 5 Part No 314105 One 50 mL bottle Elution Buffer 6 Part No 314106 One 10 mL bottle Centrifugal Concentrators Part No 309106 Pack of 10 units with 20 recovery tubes...

Page 5: ...the dry ice ethanol bath and the 37 C water bath 6 Collect the AAV supernatant by centrifugation at 10 000 x g for 10 minutes Discard the pellet 7 Optional For AAV samples produced with a helper adeno...

Page 6: ...x at 4 C for 10 minutes on an orbital shaker 17 Collect the elution fraction by centrifugation for 10 minutes at 1 000 rpm 18 Carefully remove the elution supernatant II Final Buffer Exchange and Conc...

Page 7: ...ot be used to interpret actual results Example of Results 0 20 40 60 80 100 S u p e r n a t a n t F l o w T h r o u g h W a s h E l u t i o n GFP Figure 1 Purification of AAV2 GFP AAV2 GFP was produce...

Page 8: ...rk reverses terminal chronic hepatic failure J Clin Invest doi 10 1172 JCI73137 3 Stankowska D L et al 2015 Neuroprotective effects of transcription factor Brn3b in an ocular hypertension rat model of...

Page 9: ...ANTY OF MERCHANTABILITY OR WARRANTY OF FITNESS FOR PARTICULAR PURPOSE CELL BIOLABS sole obligation and purchaser s exclusive remedy for breach of this warranty shall be at the option of CELL BIOLABS t...

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