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Assay Principle
Page 1: ...Product Manual ViraBind AAV Purification Kit Catalog Number VPK 140 10 preps FOR RESEARCH USE ONLY Not for use in diagnostic procedures...
Page 2: ...ntly higher in vitro infection rates across a variety of cells and tissues Recombinant AAV 2 and AAV DJ vectors can be purified by CsCl gradient ultracentrifugation iodixanol discontinuous gradient ul...
Page 3: ...3 Assay Principle...
Page 4: ...L sterile tubes Purification Buffer 5 Part No 314105 One 50 mL bottle Elution Buffer 6 Part No 314106 One 10 mL bottle Centrifugal Concentrators Part No 309106 Pack of 10 units with 20 recovery tubes...
Page 5: ...the dry ice ethanol bath and the 37 C water bath 6 Collect the AAV supernatant by centrifugation at 10 000 x g for 10 minutes Discard the pellet 7 Optional For AAV samples produced with a helper adeno...
Page 6: ...x at 4 C for 10 minutes on an orbital shaker 17 Collect the elution fraction by centrifugation for 10 minutes at 1 000 rpm 18 Carefully remove the elution supernatant II Final Buffer Exchange and Conc...
Page 7: ...ot be used to interpret actual results Example of Results 0 20 40 60 80 100 S u p e r n a t a n t F l o w T h r o u g h W a s h E l u t i o n GFP Figure 1 Purification of AAV2 GFP AAV2 GFP was produce...
Page 8: ...rk reverses terminal chronic hepatic failure J Clin Invest doi 10 1172 JCI73137 3 Stankowska D L et al 2015 Neuroprotective effects of transcription factor Brn3b in an ocular hypertension rat model of...
Page 9: ...ANTY OF MERCHANTABILITY OR WARRANTY OF FITNESS FOR PARTICULAR PURPOSE CELL BIOLABS sole obligation and purchaser s exclusive remedy for breach of this warranty shall be at the option of CELL BIOLABS t...
