Cell biolabs ViraBind Series Product Manual Download Page 4

 

1.

 

AAV-100: 293AAV Cell Line 

Related Products 

2.

 

VPK-141: ViraBind™ AAV Purification Mega Kit 

3.

 

VPK-145: QuickTiter™ AAV Quantitation Kit 

4.

 

AAV-200: ViraDuctin™ AAV Transduction Kit 

5.

 

VPK-099: ViraBind™ Adenovirus Miniprep Kit 

6.

 

VPK-100: ViraBind™ Adenovirus Purification Kit 

7.

 

VPK-104: ViraBind™ Lentivirus Purification Kit 

 

 

1.

 

Kit Components 

ViraBind™ AAV Reagent A

2.

 

 (Part No. 314001): One 0.3 mL sterile tube. 

ViraBind™ AAV Reagent B

3.

 

 (Part No. 314002): One 1.5 mL sterile tube. 

AAV Purification Matrix

4.

 

 (Part No. 314003): Two 1.8 mL sterile tubes. 

Purification Buffer

5.

 

 (Part No. 314105): One 50 mL bottle. 

Elution Buffer

6.

 

 (Part No. 314106): One 10 mL bottle. 

Centrifugal Concentrators

 

 (Part No. 309106): Pack of 10 units with 20 recovery tubes. 

 

1.

 

AAV Helper-Free System 

Materials Not Supplied 

2.

 

Transfection Reagent 

3.

 

HEK 293 cells and cell culture growth medium 

4.

 

15 mL conical tubes  

5.

 

Centrifuge (capable of 10,000 x g) 

 
 

Store ViraBind™ AAV Reagent B and Centrifugal Concentrators at room temperature and all other kit 
components at 4ºC until their expiration dates. 

Storage 

 

 

Remember that you will be working with samples containing infectious virus. Follow the 
recommended NIH guidelines for all materials containing BSL-2 organisms. 

Safety Considerations 

 
 

Summary of Contents for ViraBind Series

Page 1: ...Product Manual ViraBind AAV Purification Kit Catalog Number VPK 140 10 preps FOR RESEARCH USE ONLY Not for use in diagnostic procedures...

Page 2: ...ntly higher in vitro infection rates across a variety of cells and tissues Recombinant AAV 2 and AAV DJ vectors can be purified by CsCl gradient ultracentrifugation iodixanol discontinuous gradient ul...

Page 3: ...3 Assay Principle...

Page 4: ...L sterile tubes Purification Buffer 5 Part No 314105 One 50 mL bottle Elution Buffer 6 Part No 314106 One 10 mL bottle Centrifugal Concentrators Part No 309106 Pack of 10 units with 20 recovery tubes...

Page 5: ...the dry ice ethanol bath and the 37 C water bath 6 Collect the AAV supernatant by centrifugation at 10 000 x g for 10 minutes Discard the pellet 7 Optional For AAV samples produced with a helper adeno...

Page 6: ...x at 4 C for 10 minutes on an orbital shaker 17 Collect the elution fraction by centrifugation for 10 minutes at 1 000 rpm 18 Carefully remove the elution supernatant II Final Buffer Exchange and Conc...

Page 7: ...ot be used to interpret actual results Example of Results 0 20 40 60 80 100 S u p e r n a t a n t F l o w T h r o u g h W a s h E l u t i o n GFP Figure 1 Purification of AAV2 GFP AAV2 GFP was produce...

Page 8: ...rk reverses terminal chronic hepatic failure J Clin Invest doi 10 1172 JCI73137 3 Stankowska D L et al 2015 Neuroprotective effects of transcription factor Brn3b in an ocular hypertension rat model of...

Page 9: ...ANTY OF MERCHANTABILITY OR WARRANTY OF FITNESS FOR PARTICULAR PURPOSE CELL BIOLABS sole obligation and purchaser s exclusive remedy for breach of this warranty shall be at the option of CELL BIOLABS t...

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