Figure 9: MultiSplit light path diagram
3.2) Positioning the primary image (TT)
1.
Turn up your brightfield microscope light source to maximum and divert the light to the relevant side port.
2.
Ensure no filter cubes are within the MultiSplit unit.
3.
Hold a small piece of paper over the
camera mounting thread
.
4.
Close the
rectangular aperture
down until one small square is visible through the piece of paper (~one
quarter of the whole aperture).
5.
Re-position this image using the
horizontal (H) and vertical (V) image separation controls
show in
Figure 1
.
6.
When looking through the piece of paper towards the microscope from the output of the MultiSplit, move
the TT image to the bottom left corner.
3.3) Positioning the reflected images (RT, TR and RR)
Once TT is in the correct quadrant, the remaining images can then be positioned in turn.
Cube 1: Image RT
1.
Insert the calibration cube labelled Cube 1 into its correct position (note, this is the cube with the knurled
blue handle) – this will cause image RT to appear.
2.
Replace the
Cube 1
magnetic door cover
.
3.
Using the 1.5mm Allen key provided, re-position this image using
Cube 1 H & V controls
(
Figure 2
) on the
cube door. This mechanism tips and tilts the calibration cube (50% / 50% mirror), giving a smooth movement
to reposition this image.
4.
When looking through the paper towards the microscope from the output of the MultiSplit, move the RT
image to the bottom right corner.
Cube 2: Image TR
1.
Insert the calibration cube labelled Cube 2 into the cube cradle according to
Figure 6
.
2.
Insert the cube and holder into cube position 2 – this will cause image TR to appear.
3.
Replace the
Cube 2
magnetic door cover
.
