Calibration tool
Camera mounting thread
3)
Initial alignment
For initial setup, we suggest mounting the MultiSplit to your microscope without your imaging camera. The four
quadrants can then be roughly aligned in the correct configuration, which avoids issues with some imaging
cameras flipping the image in software. Final adjustments can then be made with the camera in place.
To aid this initial setup, a piece of paper held over the
camera mounting thread
is sufficient to observe the
quadrants as each cube is added to the unit.
Figure 8: Camera mounting thread minus imaging camera
3.1) Light path diagram
It is pertinent at this point to consider the internal light path within the MultiSplit and familiarise yourself with
the image nomenclature.
‘T’ refers to transmitted and similarly, ‘R’ refers to reflected.
Each of the four images is labelled accordingly:
Image reference
Related cube number
Nominal ‘colour’ of image
TT
No cubes in unit
Red
RT
Cube 1
Green
TR
Cube 2
Yellow
RR
Cube 3
Blue
