Figure 13: Auxiliary drop-in holder and retaining ring
Neutral Density kit
A series of four neutral density filters are useful if one (or more) of the channels is disproportionally brighter
than another.
03GN25: 50% Transmittance
05GN25: 32% Transmittance
06GN25: 25% Transmittance
10GN25: 10% Transmittance
Corrector lens kit
If one or more of the channels looks out of focus in comparison to others, it may be due to chromatic aberration.
This results when the focal length changes depending upon the emission wavelength and is predominantly
introduced by the objective lens. When imaging multiple wavelengths simultaneously, the phenomenon is
observed
using the MultiSplit, rather than being caused by it.
Our corrector lens kit provides a series of weak crown glass lenses, often used by trial and error for correcting
the unwanted effect. We recommend starting with the weakest lens (longest focal length) in the out of focus
channel.
IO137: Focal length 1346mm
IO138: Focal length 2019mm
IO139: Focal length 4034mm
IO140: Focal length 5000mm (1” holder required)
IO141: Focal length 10,000mm (1” holder required)
6)
Multi-depth Imaging
The MultiSplit has two auxiliary drop-in positions for each channel, accessible from the outer panels of the unit.
The corrector lens kit described in section 5.4 can be used in these positions for two purposes: a) chromatic
correction or b) to deliberately shift the focal plane for multi-depth imaging.
For precise focal plane shifting, we have a spreadsheet available to estimate the best focal length lens (if
available). Please contact us (
) for this further detail and we will be happy to advise
for your application.
The optimum positions for shifting the focal plane in each channel is indicated below.
