C.B.S. SCIENTIFIC EBU-4000 Instruction Manual Download Page 1 | Manualshive

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Semi-Dry Blotting Systems

INSTRUCTION MANUAL

EBU-4000

EBU-6000

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Summary of Contents for EBU-4000

Page 1: ...Semi Dry Blotting Systems INSTRUCTION MANUAL EBU 4000 EBU 6000...

Page 2: ...ction 5 1 2 Specifications 5 1 3 Safety 5 Section 2 Description of parts 2 1 Unpacking EBU 4000 or 6000 6 2 2 Components Assembly 6 Section 3 Instructions for Use 3 1 Blotting Unit Preparation 7 11 Se...

Page 3: ...e highest practical standards of materials workmanship and design C B S Scientific warrants that the product has been tested and will meet or exceed published specifications This warranty is valid onl...

Page 4: ...endimiento insatisfactorio atribuible a circunstancias fuera del control de C B S Scientific C B S Scientific en ning n caso asumir responsabilidad por da os incidentales o subsecuentes incluyendo en...

Page 5: ...t passes directly through the gel Use of the mask will increase transfer efficiency and reduce transfer times up to 50 1 2 Specifications Constructions Buffer chamber safety cover Acrylic Electrode pa...

Page 6: ...nts Blotting chamber with stainless steel cathode and platinum coated anode Safety cover with power leads Mylar sheets for shielding uncovered portion of anode 2 2 Components Assembly SAFETY COVER POW...

Page 7: ...is why nylon membranes are generally used uncharged nylon being preferable to positively charged nylon Nylon is the membrane of choice because it will bind DNA fragments as small as 50bp and can be ir...

Page 8: ...g on thickness 3 6mm c Cut eight sheets of 3mm blotter paper Whatman the exact size of the gel Saturate all eight sheets in running buffer d Mylar Mask Prepare a mask by cutting a rectangular hole tha...

Page 9: ...ng a rectangular hole that is slightly smaller 1 2mm than the gel dimensions b Prepare 8 pieces of Whatman 3mm chromatography paper the exact size of the gel Larger pieces of filter paper may promote...

Page 10: ...At the conclusion of the run turn power supply off and disconnect leads from blotter Cover will not release unless power cord is removed Remove the cover slowly to catch any part of the gel sandwich...

Page 11: ...lowed by the gel and 5 more pieces of saturated filter paper Again roll a glass rod over the filter paper stack to compress and remove trapped air bubbles The lid of the apparatus containing the catho...

Page 12: ...sis 86 315 327 VCH Weinheim Germany 3 Dunn S D Effects of the modification of transfer buffer composition and the renaturation of proteins in gels on the recognition of proteins on Western blots by mo...

Page 13: ...lpyrocarbonate DEPC treated water for extended periods at 37 C Electrostatic charge and heat affix the conductive platinum surface covering the titanium It can be scratched off or damaged by using sha...

Page 14: ...t Item EBU 4000 Semi Dry Blotting System Includes power leads and safety interlock 20cm x 20cm EBU 6000 Semi Dry Blotting System Includes power leads and safety interlock 35cm x 45cm MM 4000 Mylar Mas...

Page 15: ...NOTES...

Page 16: ...d online ordering www cbsscientific com E mail address sales cbssci com Mailing address C B S Scientific Company P O Box 856 Del Mar CA 92014 Shipping address C B S Scientific Company 10805 Vista Sorr...

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