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C.B.S.

œ

Scientific Co, Inc. 

13

 Semi-Dry 

Blotters 

SECTION 5 
Maintenance of Equipment 
 
5.1 Care and Handling 

 

The plastic components of the Semi-Dry Blotting units are fabricated from acrylic and polycarbonate.  
Electrodes and connectors are made from pure platinized titanium, and stainless steel.  As with any 
laboratory instrument, adequate care ensures consistent and reliable performance. 

 

After each use, rinse with de-ionized water.  Wipe dry with a soft cloth or paper towel, or allow to air 
dry.  Whenever necessary, all components may be washed gently with water and a non-abrasive 
detergent, and rinsed and dried as above.  

Never

 use abrasive cleaners, glass cleaning sprays or 

scouring pads to clean the components, as these will damage the unit and components. 

 
  Additional 

precautions: 

 

Do not autoclave or dry-heat sterilize the apparatus or components. 

  Do not expose the apparatus or components to phenol, acetone, benzene, 

halogenated hydrocarbon solvents or  alcohols. 

 

Avoid prolonged exposure of the apparatus or components to UV light. 

  Do NOT treat with diethylpyrocarbonate (DEPC)-treated water for extended 

periods at 37

°

C.   

 

Electrostatic charge and heat affix the conductive platinum surface covering the 
titanium.  It can be scratched off or damaged by using sharp objects or scouring 
pads.  This will disturb the continuity of the electric field and have a negative 
effect on transfers. 

 

The stainless electrode can be permanently damaged by corrosion if the polarity 
between anode and cathode is reversed.  CAUTION: Be certain of power 
connections before initiating transfer.   

 
 

 

5.2    Maintenance

 

The following inspection and maintenance procedures will help maintain the safety and reliable 
performance of the Blotting systems.  Replacement parts can be ordered by calling 1-858-755-
4959 or by contacting your local distributor. 

 

 

Banana plugs and power cords should be inspected regularly.  If the banana 

plugs become loose or do not feel friction tight replace the plugs or power cords.   

 

Should power cord assemblies (connectors, wire or shrouds) show any signs of 
wear or damage (e.g. cracks, nicks, abrasions, or melted insulation), replace 
them immediately. 

 

The platinum wire is secured to the banana jack by compression between a 
stainless washer and the jack nut.  The nut/washer interface should be tight and 
free of corrosion.   

 
 
 
 
 
 
 
 
 
 
 
 
 

 

 

Summary of Contents for EBU-4000

Page 1: ...Semi Dry Blotting Systems INSTRUCTION MANUAL EBU 4000 EBU 6000...

Page 2: ...ction 5 1 2 Specifications 5 1 3 Safety 5 Section 2 Description of parts 2 1 Unpacking EBU 4000 or 6000 6 2 2 Components Assembly 6 Section 3 Instructions for Use 3 1 Blotting Unit Preparation 7 11 Se...

Page 3: ...e highest practical standards of materials workmanship and design C B S Scientific warrants that the product has been tested and will meet or exceed published specifications This warranty is valid onl...

Page 4: ...endimiento insatisfactorio atribuible a circunstancias fuera del control de C B S Scientific C B S Scientific en ning n caso asumir responsabilidad por da os incidentales o subsecuentes incluyendo en...

Page 5: ...t passes directly through the gel Use of the mask will increase transfer efficiency and reduce transfer times up to 50 1 2 Specifications Constructions Buffer chamber safety cover Acrylic Electrode pa...

Page 6: ...nts Blotting chamber with stainless steel cathode and platinum coated anode Safety cover with power leads Mylar sheets for shielding uncovered portion of anode 2 2 Components Assembly SAFETY COVER POW...

Page 7: ...is why nylon membranes are generally used uncharged nylon being preferable to positively charged nylon Nylon is the membrane of choice because it will bind DNA fragments as small as 50bp and can be ir...

Page 8: ...g on thickness 3 6mm c Cut eight sheets of 3mm blotter paper Whatman the exact size of the gel Saturate all eight sheets in running buffer d Mylar Mask Prepare a mask by cutting a rectangular hole tha...

Page 9: ...ng a rectangular hole that is slightly smaller 1 2mm than the gel dimensions b Prepare 8 pieces of Whatman 3mm chromatography paper the exact size of the gel Larger pieces of filter paper may promote...

Page 10: ...At the conclusion of the run turn power supply off and disconnect leads from blotter Cover will not release unless power cord is removed Remove the cover slowly to catch any part of the gel sandwich...

Page 11: ...lowed by the gel and 5 more pieces of saturated filter paper Again roll a glass rod over the filter paper stack to compress and remove trapped air bubbles The lid of the apparatus containing the catho...

Page 12: ...sis 86 315 327 VCH Weinheim Germany 3 Dunn S D Effects of the modification of transfer buffer composition and the renaturation of proteins in gels on the recognition of proteins on Western blots by mo...

Page 13: ...lpyrocarbonate DEPC treated water for extended periods at 37 C Electrostatic charge and heat affix the conductive platinum surface covering the titanium It can be scratched off or damaged by using sha...

Page 14: ...t Item EBU 4000 Semi Dry Blotting System Includes power leads and safety interlock 20cm x 20cm EBU 6000 Semi Dry Blotting System Includes power leads and safety interlock 35cm x 45cm MM 4000 Mylar Mas...

Page 15: ...NOTES...

Page 16: ...d online ordering www cbsscientific com E mail address sales cbssci com Mailing address C B S Scientific Company P O Box 856 Del Mar CA 92014 Shipping address C B S Scientific Company 10805 Vista Sorr...

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