16
From there, the light is reflected back and
passes through the objective and eyepiece
(where it gets magnified) into the eye. This is
reflected light microscopy.
For transparent objections (e.g. protozoa), on
the other hand, the light shines from below,
through the opening in the microscope stage
and then through the object.
The light travels further through the objective
and eyepiece, where it is also magnified, and
finally goes into the eye. This is transmitted-
light microscopy.
Many microorganisms in water, many plan
components and the smallest animal parts
are already transparent in nature. Others have
to be prepared. We may make them transpar-
ent through a treatment or penetration with
the right materials (media), or by taking the
thinnest slices from them (using our hand or
a specimen slicer), and then examine them.
You can read more about this in the following
sections.
How do I make thin specimen slices?
Only do this with the supervision of your par-
ents or another adult.
As I already pointed out, the thinnest slices
possible are taken from an object. In order to
get the best results, we need some wax or
paraffin. It is best if you get a candle. Place
the wax in a pot and heat it carefully over a
low burner. Now, dip the object in the liquid
wax a few times. Then, let the wax get hard.
Using the specimen slicer (Fig. 6, 23) or a
knife/scalpel, cut the smallest slices from the
object that is covered with wax. These slices
are to be laid on a slide and covered with a
cover slip.
How do I make my own specimens?
Take the object that you want to observe and
place it on a glass slide (Fig. 6, 20). Then,
add a few drops of distilled water on the ob-
ject (Fig. 7) using a pipette (Fig. 7, 26). Now,
place a cover slip vertically at the edge of the
drop of water, so that the water runs along the
edge of the cover slip. Then, slowly lower the
cover slip over the water drops (Fig. 8).
Note:
The included glue “gum media” (Fig. 5, 21b)
is used to make permanent prepared speci-
mens. Use this in place of the distilled water.
If you want to keep the object in place on the
slide permanently, use the gum media.
How do I install the MicrOcular?
Note:
The MicrOcular only works with the includ-
ed Barlow lens! The magnification setting is
changed when the MicrOcular is used, and it
must be readjusted with the focus knob.
First, remove the Barlow lens (Fig. 4, 3) with
the current eyepiece from the eyepiece sup-
port (Fig. 4) and place the MicrOcular (Fig. 5,
4) in the eyepiece (Fig. 5).
How do I install the software?
1 Insert the CD-ROM supplied in your CD
or DVD drive. The installation menu opens
automatically. If it doesn‘t, please switch
to Windows Explorer and select your CD
drive. Start the file “setup.exe“ by double-
clicking it.
2. A choice of languages is shown. Choose
your preference. Confirm it by clicking
„OK“. At the „Welcome“ screen, click
„Next“>“.
Fig. 7
Fig. 8
2^
Fig. 4
Fig. 5
D
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E
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