13
Place a permanent slide culture (Fig. 4) directly
under the microscope lens on the plate (Fig. 4,
7) and clamp it tight. The specimen to be exam-
ined must be directly over the lighting.
Look through the eyepiece (Fig. 1, 1/2) and turn
carefully the focusing wheel (Fig. 1, 8) until you
can see a sharp picture.
Now you can get a higher magnification, while
you pull out slowly the Barlow lens (Fig. 5, 3) of
the eyepiece socket (Fig. 5, 4). With nearly en-
tirely pulled out Barlow lens the magnification
is raised to 2x.
For still higher magnification you can put the
16x eyepiece (Fig. 5, 2) into the objective re-
volver (Fig. 1, 6) and set on higher position (10x
/ 40x).
i
TIP:
Depending on the preparation
higher magnifications do not
always lead to better pictures.
With changing magnification (eyepiece or ob
-
jective lens changes, pulling out of the Barlow
lens) the sharpness of the image must be newly
defined by turning the focusing wheel (Fig. 1,
8).
NOTE:
Please be very careful when
doing this. When you move the
mechanical plate upwards to
fast the objective lens and the
slide can touch and become
damaged.
6. Viewed Object –
condition and preparation
6.1. Condition
With the Barlow lens nearly fully extended mag-
nification can be doubled. Both transparent and
non-transparent specimens can be examined
with this microscope, which is a transmitted
light model. If translucent (transparent) speci-
mens are examined the light from below goes
through the specimen, lens and eyepiece to the
eye and is magnified en route. Many small or
-
ganisms of the water, plant parts and finest an
-
imal components have now from nature these
transparent characteristic, other ones must be
accordingly prepared. Is it that we make it by
means of a pre-treatment or penetration with
suitable materials (media) transparent or thus
that we cut finest wafers off of them (hand cut,
MicroCut) and these then examine. With these
methods will us the following part make famil-
iar.
6.2. Creation of thin preparation cuts
Specimens should be sliced as thin as possible,
as stated before. A little wax or paraffin is need
-
ed to achieve the best results. A candle can be
used for the purpose. The wax is put in a bowl
and heated over a flame. The specimen is then
dipped several times in the liquid wax. The wax
is finally allowed to harden. Use a MicroCut or
knife/scalpel (carefully) to make very thin slic-
es of the object in its wax casing.
DANGER!
Be extremely careful when
using the knives/scalpels
or the MicroCut. There is an
increased risk of injury due to
the sharp edges!
These slices are then laid on a glass slide and
covered with another.
6.3. Creation of an own preparation
Put the object which shall be observed on a
glass slide and give with a pipette (Fig. 6, 17a)
a drop of distilled water on the object (Fig. 6).
Set a cover glass (in each well sorted hobby
shop available) perpendicularly at the edge of
the water drop, so that the water runs along the
cover glass edge (Fig. 7). Lower now the cover
glass slowly over the water drop.
EN
