FLUOstar / POLARstar / LUMIstar OPTIMA Operating Manual
BMG LABTECH
16/23 0413B0001I
2007-11-19
4.2.8 Bottom
Optics
The bottom optics are used to measure fluorescence, luminescence and absorbance. The bottom optics
enter the reagent box on the left side and are connected to the left position of the excitation and emission
wheels.
Figure 22: Principle drawing of bottom reading of a 96 well plate
4.3 384-Well Injection Head
A special injection head is required in order to inject into 384-well plates. The injection head is installed
similarly to an optic (see section 4.2 Installation and Changing of Optics).
This head allows injection from one pump into 384 well formats. The readings must be taken from the
bottom. Therefore, both excitation and emission positioning wheels must be switched to the bottom optics.
Place the injection needle from the desired pump into the injection head.
Figure 23: 384-well injection head, bottom reading
Figure 24: Injection in 384 well formats, top reading
Excitation
Emission
